How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells Cultured in mTeSR™ Plus
Protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as ձ™1 into mTeSR™ Plus and will cover the generation of cell aggregates and passaging protocol
This video will cover the generation of cell aggregates and describe a passaging protocol using ™ or Gentle Cell Dissociation Reagent (GCDR).
Key topics covered in this video:
- • Assessing the quality of hPSC cultures through morphology using phase-contrast microscopy to determine when to passage
- • Passaging cell aggregates using ™ or GCDR
- • Optimizing aggregate size for feeder-free PSC culture
- • Plating aggregates into maintenance medium
Video 1: Plate Coating for hPSC Cultures
Video 3: hPSC Maintenance and Morphological Assessment
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