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How to Maintain and Assess Morphology of Human Pluripotent Stem Cells Cultured in mTeSR™ Plus

Protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as ձ𳧸™1 into mTeSR™ Plus and will cover maintenance and morphological assessment of hPSC cultures in mTeSR™ Plus

In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as ձ𳧸™1, into mTeSR™ Plus.

This video will cover maintenance and morphological assessment of high-quality hPSCs cultured in mTeSR™ Plus.

Key topics covered in this video:

  • • Differences in hPSCs cultured in ձ𳧸™1 or mTeSR™ Plus
  • • How to assess quality of hPSC cultures through morphology using phase-contrast microscopy
  • • Tips to recognize and monitor spontaneous differentiation

Watch the other two videos in the series below:

Video 1: Plate Coating for hPSC Cultures
Video 2: How to Generate and Passage hPSC Aggregates


Learn more about mTeSR™ Plus hPSC maintenance media >


Publish Date: May 20, 2020