Maintenance of Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques.
Explore the resources below to support your hPSC research.
On-Demand Course: Applying the ISSCR Standards for Research
In 2023, the ISSCR published the Standards for Human Stem Cell Use in Research—a set of recommendations aimed at enhancing the reproducibility and impact of stem cell-based research worldwide. Developed in partnership with the ISSCR*, this free course will walk you through the key characterization and reporting criteria outlined in the Standards document and offer practical guidance to help you confidently apply these quality principles in your own hPSC-based projects.
*Neither the ISSCR nor the ISSCR Standards endorse any commercial products or services.
Register Now >- Reporting Practices for Publishing Results with hPSCsLearn how to plan and conduct your human pluripotent stem cell (hPSC)-based research following the ISSCR’s Standards for Human Stem Cell Use in Research
- Derivation and Applications of Human Pluripotent Stem CellsOverview of the derivation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs)
- Applying the ISSCR Standards for Research CourseLearn to confidently apply the ISSCR Standards for Human Stem Cell Use in Research to your human pluripotent stem cell-based projects.
- Advancing Neurological and Retinal Research with Genetically Diverse Human iPSC-Derived ModelsIn this webinar, Andrew Gaffney, Director of Stem Cell Manufacturing and Commercialization at º£½ÇÆƽâ°æ Technologies, showcases how high-quality, genetically diverse iPSC lines and differentiated cells are advancing research in neurological and retinal diseases. Manufactured to meet ISSCR standards, these iPSC lines undergo extensive quality control, ensuring consistency and reliability for disease modeling and drug discovery. º£½ÇÆƽâ°æ has optimized the differentiation of these iPSCs into mature, functional astrocytes and retinal pigment epithelial (RPE) cells that exhibit hallmark physiological behaviors.<br><br>As a supporter of, and participant in, ISSCR’s 2025 Annual Meeting, we share with you our presentation at ISSCR 2025. This presentation does not represent an endorsement from or support of the ISSCR.
- Transitioning from Feeder-Free Media to mTeSRâ„¢ Plus for Human Pluripotent Stem Cell CultureTransitioning human embryonic stem (ES) and induced pluripotent stem (iPS) cells from feeder-free media to mTeSRâ„¢ Plus, a serum-free, stabilized cell culture medium
- How to Coat Cultureware with Vitronectin XFâ„¢ for Pluripotent Stem Cell CultureCoating cultureware with Vitronectin XFâ„¢, which can be used for ES and iPS cells cultured in mTeSRâ„¢1, mTeSRâ„¢ Plus, or TeSRâ„¢-E8â„¢
- Enzyme-Free Passaging of Human Pluripotent Stem Cells Using ReLeSRâ„¢How to passage ES and iPS cells cultured in mTeSRâ„¢ Plus using ReLeSRâ„¢
