Maintenance of Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques.
Explore the resources below to support your hPSC research.
Survey Report: Where is Pluripotent Stem Cell Research Now?
At the beginning of 2020, º£½ÇÆƽâ°æ Technologies conducted a survey asking scientists to help highlight the needs and challenges in the hPSC field and to gather ideas on how to achieve greater reproducibility. The survey report shares some of the most interesting insights, on topics such as irreproducibility and quality control, and how to address them in your research.
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Enzyme-Free Passaging and Generation of Single Cells from Human ES and iPS Cells with Gentle Cell Dissociation ReagentGentle Cell Dissociation Reagent (GCDR) is enzyme-free, animal component-free and allows great expansion (12-40 fold) of human ES and iPS cell cultures -
Coating Plates with Matrigel® for Pluripotent Stem Cell CultureCulturing and maintaining human ESCs and iPSCs with mTeSR™1 (Catalog #5850) or TeSR™2 (Catalog #05860) requires tissue culture plates coated with a matrix -
Tap Your Way to Easy Pluripotent Stem Cell Passaging Using ReLeSRâ„¢Technical tip from our dedicated team of Product and Scientific Support specialists -
Vitronectin XFâ„¢ Defined Matrix for Human Pluripotent Stem CellsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Pluripotent Stem Cell BiologyNuclear transfer, reprogramming and cell fusion to induce pluripotency in somatic cells -
Small Molecules, Big Impact in Pluripotent Stem Cell ResearchOverview of signaling pathways and small molecules in pluripotent stem cell research -
Stem Cell States: Naive to Primed PluripotencyProperties of naive (ground) and primed pluripotent stem cells -
Using Automation to Assess the Impact of eTeSRâ„¢ on Genetic Stability of Human Pluripotent Stem CellsIn this webinar, Kimberly Snyder, a Senior Scientist in Pluripotent Stem Cell Biology at º£½ÇÆƽâ°æ Technologies, explores the critical relationship between culture methods and the long-term genetic stability of human pluripotent stem cells (hPSCs). Kimberly presents the rationale, design, and outcomes of a large-scale, automated study evaluating the genetic stability of hPSCs under single-cell passaging conditions, highlighting the development of eTeSRâ„¢, a culture medium specifically optimized to mitigate the risks associated with single-cell passaging.<br><br>As a supporter of, and participant in, ISSCR’s 2025 Annual Meeting, we share with you our presentation at ISSCR 2025. This presentation does not represent an endorsement from or support of the ISSCR.
