Maintenance of Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques.
Explore the resources below to support your hPSC research.
On-Demand Course: Applying the ISSCR Standards for Research
In 2023, the ISSCR published the Standards for Human Stem Cell Use in Research—a set of recommendations aimed at enhancing the reproducibility and impact of stem cell-based research worldwide. Developed in partnership with the ISSCR*, this free course will walk you through the key characterization and reporting criteria outlined in the Standards document and offer practical guidance to help you confidently apply these quality principles in your own hPSC-based projects.
*Neither the ISSCR nor the ISSCR Standards endorse any commercial products or services.
Register Now >- Enzyme-Free Passaging and Generation of Single Cells from Human ES and iPS Cells with Gentle Cell Dissociation ReagentGentle Cell Dissociation Reagent (GCDR) is enzyme-free, animal component-free and allows great expansion (12-40 fold) of human ES and iPS cell cultures
- Coating Plates with Matrigel® for Pluripotent Stem Cell CultureCulturing and maintaining human ESCs and iPSCs with mTeSR™1 (Catalog #5850) or TeSR™2 (Catalog #05860) requires tissue culture plates coated with a matrix
- Tap Your Way to Easy Pluripotent Stem Cell Passaging Using ReLeSRâ„¢Technical tip from our dedicated team of Product and Scientific Support specialists
- Vitronectin XFâ„¢ Defined Matrix for Human Pluripotent Stem CellsTechnical tip from our dedicated team of Product and Scientific Support specialists
- Pluripotent Stem Cell BiologyNuclear transfer, reprogramming and cell fusion to induce pluripotency in somatic cells
- Small Molecules, Big Impact in Pluripotent Stem Cell ResearchOverview of signaling pathways and small molecules in pluripotent stem cell research
- Stem Cell States: Naive to Primed PluripotencyProperties of naive (ground) and primed pluripotent stem cells
