Maintenance of Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques.
Explore the resources below to support your hPSC research.
Survey Report: Where is Pluripotent Stem Cell Research Now?
At the beginning of 2020, º£½ÇÆƽâ°æ Technologies conducted a survey asking scientists to help highlight the needs and challenges in the hPSC field and to gather ideas on how to achieve greater reproducibility. The survey report shares some of the most interesting insights, on topics such as irreproducibility and quality control, and how to address them in your research.
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mTeSRâ„¢1: The Most Published, Feeder-Independent hESC & hiPSC Maintenance MediumLearn how mTeSRâ„¢1, the most published, feeder-independent hESC and hiPSC maintenance medium, has been used by researchers around the world to make remarkable scientific advancements -
Coating Plates with Matrigel® for Pluripotent Stem Cell CultureCulturing and maintaining human ESCs and iPSCs with mTeSR™1 (Catalog #5850) or TeSR™2 (Catalog #05860) requires tissue culture plates coated with a matrix -
Development of Simplified and Defined Cell Culture for Human Pluripotent Stem CellsHuman pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques. TeSRâ„¢-E8â„¢ is a defined, xeno-free medium with a simple and published formula that was developed to meet the demands of a wide range of applications. The E8 culture system has been successfully used to derive and maintain hPSCs, and enzyme-free methods have also been developed for cell expansion and cryopreservation. More recently, defined differentiation conditions have also been established for specific lineages. These developments will provide an efficient and cost-effective E8 platform for the hPSC field. This webinar is presented by Dr. Guokai Chen at the University of Macau. -
Enzyme-Free Passaging and Generation of Single Cells from Human ES and iPS Cells with Gentle Cell Dissociation ReagentGentle Cell Dissociation Reagent (GCDR) is enzyme-free, animal component-free and allows great expansion (12-40 fold) of human ES and iPS cell cultures
