Maintenance of Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques.
Explore the resources below to support your hPSC research.
Survey Report: Where is Pluripotent Stem Cell Research Now?
At the beginning of 2020, º£½ÇÆƽâ°æ Technologies conducted a survey asking scientists to help highlight the needs and challenges in the hPSC field and to gather ideas on how to achieve greater reproducibility. The survey report shares some of the most interesting insights, on topics such as irreproducibility and quality control, and how to address them in your research.
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Using Automation to Assess the Impact of eTeSRâ„¢ on Genetic Stability of Human Pluripotent Stem CellsIn this webinar, Kimberly Snyder, a Senior Scientist in Pluripotent Stem Cell Biology at º£½ÇÆƽâ°æ Technologies, explores the critical relationship between culture methods and the long-term genetic stability of human pluripotent stem cells (hPSCs). Kimberly presents the rationale, design, and outcomes of a large-scale, automated study evaluating the genetic stability of hPSCs under single-cell passaging conditions, highlighting the development of eTeSRâ„¢, a culture medium specifically optimized to mitigate the risks associated with single-cell passaging.<br><br>As a supporter of, and participant in, ISSCR’s 2025 Annual Meeting, we share with you our presentation at ISSCR 2025. This presentation does not represent an endorsement from or support of the ISSCR. -
How to Scale Up and Differentiate hPSCs in 3D Suspension CultureJoin º£½ÇÆƽâ°æ scientists Tia Sojonky and Sarah McManus to learn all about hPSC scale-up and differentiation in 3D suspension culture. This webinar provides an in-depth look at advanced techniques for scaling up and differentiating hPSCs, with some practical tips for getting started and a 15-minute Q&A session that addresses some of the most pressing questions from the field. -
Enhancing Genetic Stability in Human Pluripotent Stem Cells Maintained As Single Cells: Introducing eTeSRâ„¢In this Innovation Showcase talk from ISSCR 2023, Dr. Adam Hirst, one of º£½ÇÆƽâ°æ’s Senior Scientists, introduces eTeSRâ„¢, a novel hPSC maintenance medium developed specifically to maintain cell quality when passaging hPSCs as single cells. -
Scaling Biomimetic Culture of Pluripotent Stem CellsHear from Dr. Maxime Feyeux, who discusses what the in vivo growth environment of pluripotent stem cells (PSCs) can teach us about in vitro culture. He summarizes some of the current challenges facing standard PSC culture technologies and shares a solution for mass producing high-quality PSCs for downstream applications. -
iPSCs As Models, Part 1: What Are Induced Pluripotent Stem Cells and How Do You Use Them?Hear from Dr. Anjana Nityanandam, Director of the Human Stem Cell Lab core facility at St. Jude Children’s Research Hospital, as she provides some introductory examples of how iPSCs are used for disease modeling and walks through how to generate and characterize iPSCs. Learn about quality control measures to improve reliability and reproducibility of data, and how to address interline variability in your experiments.
