Maintenance of Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques.
Explore the resources below to support your hPSC research.
On-Demand Course: Applying the ISSCR Standards for Research
In 2023, the ISSCR published the Standards for Human Stem Cell Use in Research—a set of recommendations aimed at enhancing the reproducibility and impact of stem cell-based research worldwide. Developed in partnership with the ISSCR*, this free course will walk you through the key characterization and reporting criteria outlined in the Standards document and offer practical guidance to help you confidently apply these quality principles in your own hPSC-based projects.
*Neither the ISSCR nor the ISSCR Standards endorse any commercial products or services.
Register Now >- How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSRâ„¢ PlusProtocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSRâ„¢1 into mTeSRâ„¢ Plus and will provide step by step instructions for plate coating
- How to Transition Human Pluripotent Stem Cells into mTeSRâ„¢ Plus from Other Feeder-Free MediaProtocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSRâ„¢1 into mTeSRâ„¢ Plus
- How to Count hPSC Aggregates to Determine Plating Density for Maintenance and DifferentiationStep-by-step instructions for counting human pluripotent stem cell (hPSC) aggregates to determine and adjust the plating densities for hPSC maintenance and differentiation
- mTeSRâ„¢1: Standardized Medium for the Feeder-Independent Maintenance of hESCs & hiPSCsHow mTeSRâ„¢1 simplifies and standardizes human embryonic & induced pluripotent stem cell cultures to minimize experimental variability
- mTeSRâ„¢1: The Most Published, Feeder-Independent hESC & hiPSC Maintenance MediumLearn how mTeSRâ„¢1, the most published, feeder-independent hESC and hiPSC maintenance medium, has been used by researchers around the world to make remarkable scientific advancements
- Tapping Technique for Passaging Pluripotent Stem Cells Using ReLeSRâ„¢ Selection and Passaging ReagentTip for the successful tapping technique when using ReLeSRâ„¢
- A Guide to Passaging Human Pluripotent Stem Cells Using mTeSRâ„¢1A guide to passaging human pluripotent stem cells using mTeSRâ„¢1
- The Role of Albumin in Feeder-Free Culture MediaDr. Hadley discusses using albumin in feeder-free culture media, and how it relates to mTeSRâ„¢1 and TeSRâ„¢-E8â„¢
