Maintenance of Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques.
Explore the resources below to support your hPSC research.
On-Demand Course: Applying the ISSCR Standards for Research
In 2023, the ISSCR published the Standards for Human Stem Cell Use in Research—a set of recommendations aimed at enhancing the reproducibility and impact of stem cell-based research worldwide. Developed in partnership with the ISSCR*, this free course will walk you through the key characterization and reporting criteria outlined in the Standards document and offer practical guidance to help you confidently apply these quality principles in your own hPSC-based projects.
*Neither the ISSCR nor the ISSCR Standards endorse any commercial products or services.
Register Now >- Development of Simplified and Defined Cell Culture for Human Pluripotent Stem CellsHuman pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques. TeSRâ„¢-E8â„¢ is a defined, xeno-free medium with a simple and published formula that was developed to meet the demands of a wide range of applications. The E8 culture system has been successfully used to derive and maintain hPSCs, and enzyme-free methods have also been developed for cell expansion and cryopreservation. More recently, defined differentiation conditions have also been established for specific lineages. These developments will provide an efficient and cost-effective E8 platform for the hPSC field. This webinar is presented by Dr. Guokai Chen at the University of Macau.
- mTeSRâ„¢1: Standardized Medium for the Feeder-Independent Maintenance of hESCs & hiPSCsHow mTeSRâ„¢1 simplifies and standardizes human embryonic & induced pluripotent stem cell cultures to minimize experimental variability
- mTeSRâ„¢1: The Most Published, Feeder-Independent hESC & hiPSC Maintenance MediumLearn how mTeSRâ„¢1, the most published, feeder-independent hESC and hiPSC maintenance medium, has been used by researchers around the world to make remarkable scientific advancements
- Tapping Technique for Passaging Pluripotent Stem Cells Using ReLeSRâ„¢ Selection and Passaging ReagentTip for the successful tapping technique when using ReLeSRâ„¢
- A Guide to Passaging Human Pluripotent Stem Cells Using mTeSRâ„¢1A guide to passaging human pluripotent stem cells using mTeSRâ„¢1
- The Role of Albumin in Feeder-Free Culture MediaDr. Hadley discusses using albumin in feeder-free culture media, and how it relates to mTeSRâ„¢1 and TeSRâ„¢-E8â„¢
