Maintenance of Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques.
Explore the resources below to support your hPSC research.
On-Demand Course: Applying the ISSCR Standards for Research
In 2023, the ISSCR published the Standards for Human Stem Cell Use in Research—a set of recommendations aimed at enhancing the reproducibility and impact of stem cell-based research worldwide. Developed in partnership with the ISSCR*, this free course will walk you through the key characterization and reporting criteria outlined in the Standards document and offer practical guidance to help you confidently apply these quality principles in your own hPSC-based projects.
*Neither the ISSCR nor the ISSCR Standards endorse any commercial products or services.
Register Now >- Using Automation to Assess the Impact of eTeSRâ„¢ on Genetic Stability of Human Pluripotent Stem CellsIn this webinar, Kimberly Snyder, a Senior Scientist in Pluripotent Stem Cell Biology at º£½ÇÆƽâ°æ Technologies, explores the critical relationship between culture methods and the long-term genetic stability of human pluripotent stem cells (hPSCs). Kimberly presents the rationale, design, and outcomes of a large-scale, automated study evaluating the genetic stability of hPSCs under single-cell passaging conditions, highlighting the development of eTeSRâ„¢, a culture medium specifically optimized to mitigate the risks associated with single-cell passaging.<br><br>As a supporter of, and participant in, ISSCR’s 2025 Annual Meeting, we share with you our presentation at ISSCR 2025. This presentation does not represent an endorsement from or support of the ISSCR.
- How to Scale Up and Differentiate hPSCs in 3D Suspension CultureJoin º£½ÇÆƽâ°æ scientists Tia Sojonky and Sarah McManus to learn all about hPSC scale-up and differentiation in 3D suspension culture. This webinar provides an in-depth look at advanced techniques for scaling up and differentiating hPSCs, with some practical tips for getting started and a 15-minute Q&A session that addresses some of the most pressing questions from the field.
- Enhancing Genetic Stability in hPSC Cultures: Introducing eTeSRâ„¢ for Single-Cell hPSC MaintenanceIn this Innovation Showcase talk from ISSCR 2023, Dr. Adam Hirst, one of º£½ÇÆƽâ°æ’s Senior Scientists, introduces eTeSRâ„¢, a novel hPSC maintenance medium developed specifically to maintain cell quality when passaging hPSCs as single cells.
- Scaling Biomimetic Culture of Pluripotent Stem CellsHear from Dr. Maxime Feyeux, who discusses what the in vivo growth environment of pluripotent stem cells (PSCs) can teach us about in vitro culture. He summarizes some of the current challenges facing standard PSC culture technologies and shares a solution for mass producing high-quality PSCs for downstream applications.
- Quality by Design: Reagents and Support for hPSC-Derived Cell and Gene TherapiesEach year, more human pluripotent stem cell-derived (hPSC-derived) therapies move towards the clinic, and º£½ÇÆƽâ°æ is committed to supporting researchers from discovery to clinical application. In this Innovation Showcase from ISSCR 2021, Lynn Csontos will demonstrate how we build quality into our products, processes, and policies to support your project timing, product quality, and overall vision. Kimberly Snyder will then present data that shows how TeSRâ„¢-AOF, a novel animal origin-free (AOF) hPSC maintenance medium, can support optimized cell quality, improved performance and reproducibility across all cell lines.
- How to Maintain and Assess Morphology of Human Pluripotent Stem Cells Cultured in mTeSRâ„¢ PlusProtocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSRâ„¢1 into mTeSRâ„¢ Plus and will cover maintenance and morphological assessment of hPSC cultures in mTeSRâ„¢ Plus
