Maintenance of Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques.
Explore the resources below to support your hPSC research.
Survey Report: Where is Pluripotent Stem Cell Research Now?
At the beginning of 2020, º£½ÇÆƽâ°æ Technologies conducted a survey asking scientists to help highlight the needs and challenges in the hPSC field and to gather ideas on how to achieve greater reproducibility. The survey report shares some of the most interesting insights, on topics such as irreproducibility and quality control, and how to address them in your research.
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How to Maintain and Assess Morphology of Human Pluripotent Stem Cells (hPSCs) in mTeSRâ„¢ PlusProtocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSRâ„¢1 into mTeSRâ„¢ Plus and will cover maintenance and morphological assessment of hPSC cultures in mTeSRâ„¢ Plus -
How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSRâ„¢ PlusProtocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSRâ„¢1 into mTeSRâ„¢ Plus and will cover the generation of cell aggregates and passaging protocol -
How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSRâ„¢ PlusProtocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSRâ„¢1 into mTeSRâ„¢ Plus and will provide step by step instructions for plate coating -
How to Transition Human Pluripotent Stem Cells into mTeSRâ„¢ Plus from Other Feeder-Free MediaProtocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSRâ„¢1 into mTeSRâ„¢ Plus -
How to Count hPSC Aggregates to Determine Plating Density for Maintenance and DifferentiationStep-by-step instructions for counting human pluripotent stem cell (hPSC) aggregates to determine and adjust the plating densities for hPSC maintenance and differentiation
