Maintenance of Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques.
Explore the resources below to support your hPSC research.
On-Demand Course: Applying the ISSCR Standards for Research
In 2023, the ISSCR published the Standards for Human Stem Cell Use in Research—a set of recommendations aimed at enhancing the reproducibility and impact of stem cell-based research worldwide. Developed in partnership with the ISSCR*, this free course will walk you through the key characterization and reporting criteria outlined in the Standards document and offer practical guidance to help you confidently apply these quality principles in your own hPSC-based projects.
*Neither the ISSCR nor the ISSCR Standards endorse any commercial products or services.
Register Now >- Quality by Design: Reagents and Support for hPSC-Derived Cell and Gene TherapiesEach year, more human pluripotent stem cell-derived (hPSC-derived) therapies move towards the clinic, and º£½ÇÆƽâ°æ is committed to supporting researchers from discovery to clinical application. In this Innovation Showcase from ISSCR 2021, Lynn Csontos will demonstrate how we build quality into our products, processes, and policies to support your project timing, product quality, and overall vision. Kimberly Snyder will then present data that shows how TeSRâ„¢-AOF, a novel animal origin-free (AOF) hPSC maintenance medium, can support optimized cell quality, improved performance and reproducibility across all cell lines.
- How to Maintain and Assess Morphology of Human Pluripotent Stem Cells (hPSCs) in mTeSRâ„¢ PlusProtocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSRâ„¢1 into mTeSRâ„¢ Plus and will cover maintenance and morphological assessment of hPSC cultures in mTeSRâ„¢ Plus
- How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSRâ„¢ PlusProtocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSRâ„¢1 into mTeSRâ„¢ Plus and will cover the generation of cell aggregates and passaging protocol
- How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSRâ„¢ PlusProtocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSRâ„¢1 into mTeSRâ„¢ Plus and will provide step by step instructions for plate coating
- How to Transition Human Pluripotent Stem Cells into mTeSRâ„¢ Plus from Other Feeder-Free MediaProtocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSRâ„¢1 into mTeSRâ„¢ Plus
- How to Count hPSC Aggregates to Determine Plating Density for Maintenance and DifferentiationStep-by-step instructions for counting human pluripotent stem cell (hPSC) aggregates to determine and adjust the plating densities for hPSC maintenance and differentiation
