Hematopoietic Stem and Progenitor Cell Research
The defining properties of multipotent hematopoietic stem cells (HSCs) are their ability to differentiate into multiple lymphoid and myeloid lineages, as well as to self-renew to ensure that the HSC pool is not depleted over the lifespan of an individual. As HSCs proliferate, they progress through a series of lineage commitment steps, producing hematopoietic progenitor cells (HPCs) that are shorter-lived and more restricted in their differentiation potential.
The screening of novel compounds to predict potential toxicity to the hematopoietic system (i.e. hematotoxicity) is an important step in drug development. HemaToxâ„¢ assays can assess the toxicity of drugs on the growth and lineage-specific differentiation of human CD34+ hematopoietic stem and progenitor cells (HSPCs) into one of three specific progenitor cell lineages (erythroid, myeloid, or megakaryocyte).
Explore our scientific resources below to learn more about how to source, culture, and analyze HSPCs.
Procedure for Setting Up the CFU Assay
This video provides step-by-step guidelines for setting up hematopoietic colony-forming unit (CFU) assays using methylcellulose-based MethoCultâ„¢ medium. Steps include preparing cells, plating semi-solid medium and incubating.
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Colony-Forming Unit Assays with Mouse Cellsº£½ÇÆƽâ°æ Technologies offers a variety of MethoCult methylcellulose-based semi-solid media products to be used with mouse cells for colony forming unit (CFU) assays -
Human Hematopoietic Stem and Progenitor Cell Markers and Phenotyping PanelsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Isolating Hematopoietic Progenitors Using EasySepâ„¢, RoboSepâ„¢, RosetteSepâ„¢ and SepMateâ„¢Technical tip from our dedicated team of Product and Scientific Support specialists -
Modeling Alzheimer's risk using human TREM2-knockout microgliaAmanda McQuade from Dr. Mathew Blurton-Jones’s lab discusses her protocol for differentiating microglia from induced pluripotent stem cells (iPSCs) and the use of these microglia in vivo and in vitro to uncover the mechanisms of immune activation and neurodegeneration in Alzheimer’s disease. -
Identification of Colonies Derived from Mouse Hematopoietic ProgenitorsRepresentative colony images and tips for identifying progenitor subtypes in CFU assays -
Maturation of Culture-Expanded Erythroid Progenitor CellsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Bone Marrow Niches and HSC FatesA detailed reference on signaling pathways in the bone marrow and how these influence HSC fate decisions; created in partnership with Nature Reviews Immunology and Nature Reviews Molecular Cell Biology
