Hematopoietic Stem and Progenitor Cell Research
The defining properties of multipotent hematopoietic stem cells (HSCs) are their ability to differentiate into multiple lymphoid and myeloid lineages, as well as to self-renew to ensure that the HSC pool is not depleted over the lifespan of an individual. As HSCs proliferate, they progress through a series of lineage commitment steps, producing hematopoietic progenitor cells (HPCs) that are shorter-lived and more restricted in their differentiation potential.
The screening of novel compounds to predict potential toxicity to the hematopoietic system (i.e. hematotoxicity) is an important step in drug development. HemaToxâ„¢ assays can assess the toxicity of drugs on the growth and lineage-specific differentiation of human CD34+ hematopoietic stem and progenitor cells (HSPCs) into one of three specific progenitor cell lineages (erythroid, myeloid, or megakaryocyte).
Explore our scientific resources below to learn more about how to source, culture, and analyze HSPCs.
Procedure for Setting Up the CFU Assay
This video provides step-by-step guidelines for setting up hematopoietic colony-forming unit (CFU) assays using methylcellulose-based MethoCultâ„¢ medium. Steps include preparing cells, plating semi-solid medium and incubating.
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Determining the Potency & Stability of Hematopoietic CellsIn this webinar, Dr. Jackie Damen of º£½ÇÆƽâ°æ Technologies describes the effects of cryopreservation protocols on the engraftment potential of hematopoietic cells and the need for standardized potency assays, including the Colony-Forming Unit (CFU) assay, for hematopoietic cell products. -
CFU Assay Instructions for Global Proficiency Testing ProgramsLearn to perform the colony-forming unit (CFU) assay for Proficiency Testing programs -
Daniel Gray, PhDDr. Daniel Gray describes his work studying cell death and the potential applications in drug development for immune disorders -
Courteney LaiCourteney Lai describes her work in the Humphries' lab studying leukemic stem cell function using models of acute myeloid leukemia -
Evaluating Hematopoietic Cell Therapy Product Potency Using the CFU AssayTechnical tip from our dedicated team of Product and Scientific Support specialists -
Expansion of CD34+ Cells in Serum-Free CultureTechnical tip from our dedicated team of Product and Scientific Support specialists -
Frequently Asked Questions on Primary CellsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Isolating Hematopoietic Progenitors Using EasySepâ„¢, RoboSepâ„¢, RosetteSepâ„¢ and SepMateâ„¢Technical tip from our dedicated team of Product and Scientific Support specialists
