Hematopoietic Stem and Progenitor Cell Research
The defining properties of multipotent hematopoietic stem cells (HSCs) are their ability to differentiate into multiple lymphoid and myeloid lineages, as well as to self-renew to ensure that the HSC pool is not depleted over the lifespan of an individual. As HSCs proliferate, they progress through a series of lineage commitment steps, producing hematopoietic progenitor cells (HPCs) that are shorter-lived and more restricted in their differentiation potential.
The screening of novel compounds to predict potential toxicity to the hematopoietic system (i.e. hematotoxicity) is an important step in drug development. HemaToxâ„¢ assays can assess the toxicity of drugs on the growth and lineage-specific differentiation of human CD34+ hematopoietic stem and progenitor cells (HSPCs) into one of three specific progenitor cell lineages (erythroid, myeloid, or megakaryocyte).
Explore our scientific resources below to learn more about how to source, culture, and analyze HSPCs.
Procedure for Setting Up the CFU Assay
This video provides step-by-step guidelines for setting up hematopoietic colony-forming unit (CFU) assays using methylcellulose-based MethoCultâ„¢ medium. Steps include preparing cells, plating semi-solid medium and incubating.
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“Translating Stem Cell Discoveries†Featuring Dr. Ilyas SingeçOn this episode of the Stem Cell Podcast, Dr. Ilyas Singeç from National Center for Advancing Translational Sciences discusses standardizing stem cell differentiation, high-throughput cell culture, personalized medicine, and the potential for biomanufacturing in space. -
Determining the Potency & Stability of Hematopoietic CellsIn this webinar, Dr. Jackie Damen of º£½ÇÆƽâ°æ Technologies describes the effects of cryopreservation protocols on the engraftment potential of hematopoietic cells and the need for standardized potency assays, including the Colony-Forming Unit (CFU) assay, for hematopoietic cell products. -
CFU Assay Instructions for Global Proficiency Testing ProgramsLearn to perform the colony-forming unit (CFU) assay for Proficiency Testing programs -
Culturing Leukemic Stem & Progenitor Cells with StemSpanâ„¢ MediumProtocol Describing How to Culture Chronic Myeloid Leukemia Cells with StemSpanâ„¢ Serum-Free Expansion Medium II (SFEM II) -
Daniel Gray, PhDDr. Daniel Gray describes his work studying cell death and the potential applications in drug development for immune disorders -
Lost in Translation - Moving Your Research to Clinical TrialsThis webinar describes steps and considerations involved in translating research to the clinic. -
Hematopoietic Colony Enumeration and Identification: A Brief OverviewOverview of the different classes of hematopoietic progenitors detected in colony-forming unit (CFU) assays using various formulations of MethoCultâ„¢ methylcellulose-based media -
How to Set Up the CFU Assay for Custom Hematopoietic Training Courses Using SmartDishâ„¢Learn how to perform all steps involved in the colony-forming unit (CFU) assay setup for custom hematopoietic training courses using SmartDishâ„¢ cultureware -
CFU Assay Setup for Custom Hematopoietic Training CoursesLearn how to perform all steps involved in the CFU assay setup using 35mm dishes for custom hematopoietic training courses
