Hematopoietic Stem and Progenitor Cell Research
The defining properties of multipotent hematopoietic stem cells (HSCs) are their ability to differentiate into multiple lymphoid and myeloid lineages, as well as to self-renew to ensure that the HSC pool is not depleted over the lifespan of an individual. As HSCs proliferate, they progress through a series of lineage commitment steps, producing hematopoietic progenitor cells (HPCs) that are shorter-lived and more restricted in their differentiation potential.
The screening of novel compounds to predict potential toxicity to the hematopoietic system (i.e. hematotoxicity) is an important step in drug development. HemaToxâ„¢ assays can assess the toxicity of drugs on the growth and lineage-specific differentiation of human CD34+ hematopoietic stem and progenitor cells (HSPCs) into one of three specific progenitor cell lineages (erythroid, myeloid, or megakaryocyte).
Explore our scientific resources below to learn more about how to source, culture, and analyze HSPCs.
Procedure for Setting Up the CFU Assay
This video provides step-by-step guidelines for setting up hematopoietic colony-forming unit (CFU) assays using methylcellulose-based MethoCultâ„¢ medium. Steps include preparing cells, plating semi-solid medium and incubating.
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“Translating Stem Cell Discoveries†Featuring Dr. Ilyas SingeçOn this episode of the Stem Cell Podcast, Dr. Ilyas Singeç from National Center for Advancing Translational Sciences discusses standardizing stem cell differentiation, high-throughput cell culture, personalized medicine, and the potential for biomanufacturing in space. -
Courteney LaiCourteney Lai describes her work in the Humphries' lab studying leukemic stem cell function using models of acute myeloid leukemia -
David Knapp, PhDDr. David Knapp shares his work examining how growth factors affect the survival, proliferation, and maintenance of cells of the cord blood HSC compartment -
Daniel Gray, PhDDr. Daniel Gray describes his work studying cell death and the potential applications in drug development for immune disorders -
Culturing Leukemic Stem & Progenitor Cells with StemSpanâ„¢ MediumProtocol Describing How to Culture Chronic Myeloid Leukemia Cells with StemSpanâ„¢ Serum-Free Expansion Medium II (SFEM II) -
Evaluating Hematopoietic Cell Therapy Product Potency Using the CFU AssayTechnical tip from our dedicated team of Product and Scientific Support specialists -
CFU Assay Instructions for Global Proficiency Testing ProgramsLearn to perform the colony-forming unit (CFU) assay for Proficiency Testing programs
