Hematopoietic Stem and Progenitor Cell Research
The defining properties of multipotent hematopoietic stem cells (HSCs) are their ability to differentiate into multiple lymphoid and myeloid lineages, as well as to self-renew to ensure that the HSC pool is not depleted over the lifespan of an individual. As HSCs proliferate, they progress through a series of lineage commitment steps, producing hematopoietic progenitor cells (HPCs) that are shorter-lived and more restricted in their differentiation potential.
The screening of novel compounds to predict potential toxicity to the hematopoietic system (i.e. hematotoxicity) is an important step in drug development. HemaToxâ„¢ assays can assess the toxicity of drugs on the growth and lineage-specific differentiation of human CD34+ hematopoietic stem and progenitor cells (HSPCs) into one of three specific progenitor cell lineages (erythroid, myeloid, or megakaryocyte).
Explore our scientific resources below to learn more about how to source, culture, and analyze HSPCs.
Procedure for Setting Up the CFU Assay
This video provides step-by-step guidelines for setting up hematopoietic colony-forming unit (CFU) assays using methylcellulose-based MethoCultâ„¢ medium. Steps include preparing cells, plating semi-solid medium and incubating.
View Now >- Serum- and Feeder-Free Differentiation of Erythroid Progenitor Cells from hPSCsIn this presentation from ASGCT 2021, Selena Hallahan and Crystal Chau describe how erythroid progenitors can be generated from hPSCs, with potential applications in drug discovery and disease modeling.
- Lost in Translation - Moving Your Research to Clinical TrialsThis webinar describes steps and considerations involved in translating research to the clinic.
- Modeling Alzheimer's risk using human TREM2-knockout microgliaAmanda McQuade from Dr. Mathew Blurton-Jones’s lab discusses her protocol for differentiating microglia from induced pluripotent stem cells (iPSCs) and the use of these microglia in vivo and in vitro to uncover the mechanisms of immune activation and neurodegeneration in Alzheimer’s disease.
- New Tools for the Ex Vivo Expansion of Human Hematopoietic Stem and Progenitor CellsThe development of standardized ex vivo HSPC expansion methods is needed to generate clinically relevant cell numbers for transplantation. This webinar will review key concepts in human HSPC biology, including standardized methods and recently demonstrated strategies for expanding HSPCs in culture. Additionally, º£½ÇÆƽâ°æ products for the entire workflow of HSPC expansion will be covered, from isolation to expansion and differentiation, and analysis.
