Hematopoietic Stem and Progenitor Cell Research
The defining properties of multipotent hematopoietic stem cells (HSCs) are their ability to differentiate into multiple lymphoid and myeloid lineages, as well as to self-renew to ensure that the HSC pool is not depleted over the lifespan of an individual. As HSCs proliferate, they progress through a series of lineage commitment steps, producing hematopoietic progenitor cells (HPCs) that are shorter-lived and more restricted in their differentiation potential.
The screening of novel compounds to predict potential toxicity to the hematopoietic system (i.e. hematotoxicity) is an important step in drug development. HemaToxâ„¢ assays can assess the toxicity of drugs on the growth and lineage-specific differentiation of human CD34+ hematopoietic stem and progenitor cells (HSPCs) into one of three specific progenitor cell lineages (erythroid, myeloid, or megakaryocyte).
Explore our scientific resources below to learn more about how to source, culture, and analyze HSPCs.
Procedure for Setting Up the CFU Assay
This video provides step-by-step guidelines for setting up hematopoietic colony-forming unit (CFU) assays using methylcellulose-based MethoCultâ„¢ medium. Steps include preparing cells, plating semi-solid medium and incubating.
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Expansion of CD34+ Cells in Serum-Free CultureTechnical tip from our dedicated team of Product and Scientific Support specialists -
Isolating Hematopoietic Progenitors Using EasySepâ„¢, RoboSepâ„¢, RosetteSepâ„¢ and SepMateâ„¢Technical tip from our dedicated team of Product and Scientific Support specialists -
Colony-Forming Unit Assays with Mouse Cellsº£½ÇÆƽâ°æ Technologies offers a variety of MethoCult methylcellulose-based semi-solid media products to be used with mouse cells for colony forming unit (CFU) assays -
Maintaining Humidity in CFU Assays using MethoCultâ„¢ MediaTechnical tip from our dedicated team of Product and Scientific Support specialists -
Cord Blood CD34+ Cell IsolationTechnical tip from our dedicated team of Product and Scientific Support specialists -
Human Hematopoietic Stem and Progenitor Cell Markers and Phenotyping PanelsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Serum-Free Expansion of CD34+ Cells with SFEM II and UM729Explore the use of StemSpanâ„¢ SFEM II medium, StemSpanâ„¢ CD34+ Expansion Supplement and small molecules UM171 or UM729 to enhance expansion of HSPCs -
Maturation of Culture-Expanded Erythroid Progenitor CellsTechnical tip from our dedicated team of Product and Scientific Support specialists
