Hematopoietic Stem and Progenitor Cell Research
The defining properties of multipotent hematopoietic stem cells (HSCs) are their ability to differentiate into multiple lymphoid and myeloid lineages, as well as to self-renew to ensure that the HSC pool is not depleted over the lifespan of an individual. As HSCs proliferate, they progress through a series of lineage commitment steps, producing hematopoietic progenitor cells (HPCs) that are shorter-lived and more restricted in their differentiation potential.
The screening of novel compounds to predict potential toxicity to the hematopoietic system (i.e. hematotoxicity) is an important step in drug development. HemaToxâ„¢ assays can assess the toxicity of drugs on the growth and lineage-specific differentiation of human CD34+ hematopoietic stem and progenitor cells (HSPCs) into one of three specific progenitor cell lineages (erythroid, myeloid, or megakaryocyte).
Explore our scientific resources below to learn more about how to source, culture, and analyze HSPCs.
Procedure for Setting Up the CFU Assay
This video provides step-by-step guidelines for setting up hematopoietic colony-forming unit (CFU) assays using methylcellulose-based MethoCultâ„¢ medium. Steps include preparing cells, plating semi-solid medium and incubating.
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Bone Marrow Niches and HSC FatesA detailed reference on signaling pathways in the bone marrow and how these influence HSC fate decisions; created in partnership with Nature Reviews Immunology and Nature Reviews Molecular Cell Biology -
Expansion of CD34+ Cells in Serum-Free CultureTechnical tip from our dedicated team of Product and Scientific Support specialists -
Cord Blood CD34+ Cell IsolationTechnical tip from our dedicated team of Product and Scientific Support specialists -
Maintaining Humidity in CFU Assays using MethoCultâ„¢ MediaTechnical tip from our dedicated team of Product and Scientific Support specialists -
"Blood Cells and the Bone Marrow" Featuring Dr. Dominique BonnetOn this episode of the Stem Cell Podcast, Dr. Dominique Bonnet from the Francis Crick Institute discusses her research into why and how acute myeloid leukemia develops in the bone marrow. -
Mouse Hematopoietic Stem and Progenitor Cell PhenotypingOverview of mouse HSPC subset surface markers and frequencies -
Human Hematopoietic Stem and Progenitor Cell PhenotypingOverview of subset surface markers, frequencies and assays for analysis -
Small Molecules, Big Impact in Cancer ResearchOverview of signaling pathways and small molecules in cancer research -
Identification of Colonies Derived from Human Hematopoietic ProgenitorsRepresentative colony images and tips for identifying progenitor subtypes in CFU assays
