Hematopoietic Stem and Progenitor Cell Research
The defining properties of multipotent hematopoietic stem cells (HSCs) are their ability to differentiate into multiple lymphoid and myeloid lineages, as well as to self-renew to ensure that the HSC pool is not depleted over the lifespan of an individual. As HSCs proliferate, they progress through a series of lineage commitment steps, producing hematopoietic progenitor cells (HPCs) that are shorter-lived and more restricted in their differentiation potential.
The screening of novel compounds to predict potential toxicity to the hematopoietic system (i.e. hematotoxicity) is an important step in drug development. HemaToxâ„¢ assays can assess the toxicity of drugs on the growth and lineage-specific differentiation of human CD34+ hematopoietic stem and progenitor cells (HSPCs) into one of three specific progenitor cell lineages (erythroid, myeloid, or megakaryocyte).
Explore our scientific resources below to learn more about how to source, culture, and analyze HSPCs.
Procedure for Setting Up the CFU Assay
This video provides step-by-step guidelines for setting up hematopoietic colony-forming unit (CFU) assays using methylcellulose-based MethoCultâ„¢ medium. Steps include preparing cells, plating semi-solid medium and incubating.
View Now >- Cord Blood CD34+ Cell IsolationTechnical tip from our dedicated team of Product and Scientific Support specialists
- Human Hematopoietic Stem and Progenitor Cell Markers and Phenotyping PanelsTechnical tip from our dedicated team of Product and Scientific Support specialists
- Serum-Free Expansion of CD34+ Cells with SFEM II and UM729Explore the use of StemSpanâ„¢ SFEM II medium, StemSpanâ„¢ CD34+ Expansion Supplement and small molecules UM171 or UM729 to enhance expansion of HSPCs
- Maturation of Culture-Expanded Erythroid Progenitor CellsTechnical tip from our dedicated team of Product and Scientific Support specialists
- Culture and Analysis of Hematopoietic Progenitor Cells in CFU AssaysUse these tips to better understand how the right medium can make the analysis of your hematopoietic CFU assays clearer
- Extending the Life of a CFU AssayTechnical tip from our dedicated team of Product and Scientific Support specialists
- Frequently Asked Questions on Primary CellsTechnical tip from our dedicated team of Product and Scientific Support specialists
- Identification of Colonies Derived from Human Hematopoietic ProgenitorsRepresentative colony images and tips for identifying progenitor subtypes in CFU assays
- Identification of Colonies Derived from Mouse Hematopoietic ProgenitorsRepresentative colony images and tips for identifying progenitor subtypes in CFU assays
- Small Molecules, Big Impact in Cancer ResearchOverview of signaling pathways and small molecules in cancer research
- Human Hematopoietic Stem and Progenitor Cell PhenotypingOverview of subset surface markers, frequencies and assays for analysis
