Hematopoietic Stem and Progenitor Cell Research
The defining properties of multipotent hematopoietic stem cells (HSCs) are their ability to differentiate into multiple lymphoid and myeloid lineages, as well as to self-renew to ensure that the HSC pool is not depleted over the lifespan of an individual. As HSCs proliferate, they progress through a series of lineage commitment steps, producing hematopoietic progenitor cells (HPCs) that are shorter-lived and more restricted in their differentiation potential.
The screening of novel compounds to predict potential toxicity to the hematopoietic system (i.e. hematotoxicity) is an important step in drug development. HemaToxâ„¢ assays can assess the toxicity of drugs on the growth and lineage-specific differentiation of human CD34+ hematopoietic stem and progenitor cells (HSPCs) into one of three specific progenitor cell lineages (erythroid, myeloid, or megakaryocyte).
Explore our scientific resources below to learn more about how to source, culture, and analyze HSPCs.
Procedure for Setting Up the CFU Assay
This video provides step-by-step guidelines for setting up hematopoietic colony-forming unit (CFU) assays using methylcellulose-based MethoCultâ„¢ medium. Steps include preparing cells, plating semi-solid medium and incubating.
View Now >- Bone Marrow Niches and HSC FatesA detailed reference on signaling pathways in the bone marrow and how these influence HSC fate decisions; created in partnership with Nature Reviews Immunology and Nature Reviews Molecular Cell Biology
- Mouse Hematopoietic Stem and Progenitor Cell PhenotypingOverview of mouse HSPC subset surface markers and frequencies
- How to Prepare and Plate Semi-Solid MediumSteps involved with preparing and plating methylcellulose-based semi-solid media (e.g. MethoCultâ„¢ and ClonaCellâ„¢)
- STEMvisionâ„¢ Automated and Standardized CFC EnumerationFeatures and benefits of the STEMvisionâ„¢ system, the world's first automated instrument and computer system for imaging and scoring hematopoietic colonies in the colony-forming cell (CFC) assay
- How to Deplete Red Blood Cells from Small Volume Samples with HetaSepâ„¢How to use an erythrocyte aggregation agent to remove red blood cells (RBCs) from small volume blood samples prior to colony-forming unit (CFU) assays
- StemSpanâ„¢ Serum-Free Expansion MediaLearn about StemSpanâ„¢ media and StemSpanâ„¢ cytokine cocktails for expansion of hematopoietic stem and progenitor cells
- How to Isolate Nucleated Cells from Whole Blood Using HetaSepâ„¢ Erythrocyte Aggregation AgentHetaSepâ„¢ is an erythrocyte aggregation agent used to quickly separate nucleated cells from red blood cells
