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Figure 1. STEMprep™ Mouse Lung Dissociation Kit Achieves High Cell Viability and Yield

Mouse lung tissue was processed into single-cell suspensions using the STEMprep™ Mouse Lung Dissociation Kit and the STEMprep™ Tissue Dissociator, an alternative automated system, or a manual dissociation method. (A) Viability of total nucleated cells. (B) Yield of viable cells per whole lung tissue (251 - 495 mg). (C and D) Proportion and yield of CD45+ immune, EpCAM+ epithelial, and CD31+ endothelial cells. Viability, yield, and subset composition were assessed by flow cytometry. Red blood cells were lysed with ammonium chloride solution before analysis. Data are presented as mean ± SD (n = 20), * p < 0.05, one-way ANOVA with Tukey's multiple comparisons test.

Figure 2. STEMprep™-Processed Mouse Lung Macrophages Are Phagocytic and Produce Cytokines upon Activation

Mouse lung tissue was processed into single-cell suspensions using the STEMprep™ Mouse Lung Dissociation Kit and the STEMprep™ Tissue Dissociator, an alternative automated system, or a manual dissociation method. (A) Lung F4/80+ macrophages were isolated from the single-cell suspensions using EasySep™ Mouse F4/80 Positive Selection Kit. (B) The isolated F4/80+ cells were incubated for 2 hours in the presence of pHrodo™ Green-conjugated E. coli BioParticles™ at 2 - 8°C (Cold) or 37°C. The fluorescence of phagocytosed BioParticles™ was measured by flow cytometry. (C) Intracellular flow cytometry staining of TNF-ɑ production by F4/80+ macrophages cultured overnight in the presence of 3 µg/mL Brefeldin A and treated with (+) or without (-) 100 ng/mL of lipopolysaccharides (LPS). Data are presented as mean ± SD (n = 4 - 6).