³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Mouse Liver Dissociation Kit
Generate viable single-cell suspensions from mouse liver tissue with the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociation Kit and ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator
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³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue DissociatorAutomate tissue dissociation with ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ to reliably achieve high-yield, high-viability cell suspensions for your research
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³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Sample Tubes³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Sample Tubes enable consistent tissue dissociation with the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator to support high-quality single-cell preparation
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Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells
Overview
The process is specifically designed to yield high numbers of non-parenchymal cells, including liver sinusoidal endothelial cells (LSECs) and Kupffer cells. By combining enzymatic digestion of the extracellular matrix with controlled mechanical dissociation, this method ensures consistent results with minimal hands-on time and user variability.
After dissociation, the cell suspension can be easily filtered and optionally treated with a debris removal step to improve sample purity. The resulting single-cell suspension is immediately suitable for downstream applications such as cell separation, culture, flow cytometry, and functional, genetic, or proteomic studies.
For best results, use this kit in conjunction with the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator and ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Sample Tubes.
For more information on ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢, visit the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ overview page. Additionally, explore our instrumentation overview page or download our to learn more about available service options, including warranty coverage and additional support packages.
Data Figures
Figure 1. ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Mouse Liver Dissociation Kit Achieves High Cell Viability and Consistent Yield
Mouse liver tissue was processed into single-cell suspensions using the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Mouse Liver Dissociation Kit and the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator or an alternative automated system. (A) Viability of total nucleated cells. (B) Yield of viable cells per whole liver tissue (900 - 2000 mg). (C and D) Proportion and yield of F4/80+CLEC4F+ Kupffer cells, and CD31+ liver sinusoidal endothelial cells. Viability, yield, and subset composition were assessed by flow cytometry. Red blood cells were lysed with ammonium chloride solution before subset analysis. Data are presented as mean ± SD (n = 15 - 37).
Figure 2. ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢-Processed Mouse Kupffer Cells Maintain Phagocytic Function
Mouse liver tissue was dissociated into single-cell suspensions using the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Mouse Liver Dissociation Kit and the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator, or an alternative automated system. (A) Kupffer cells were isolated from the single-cell suspensions using the EasySepâ„¢ Mouse F4/80 Positive Selection Kit. (B) The isolated F4/80+CLEC4F+ cells were incubated for 2h in the presence of pHrodoâ„¢ Green-conjugated E. coli BioParticlesâ„¢ (BP) at 2 - 8°C (Cold) or 37°C. The fluorescence of phagocytosed BP was measured by flow cytometry. Data are presented as mean ± SD (n = 6).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (5)
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³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Mouse Liver Dissociation Kit
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT º£½ÇÆÆ½â°æ, REFER TO WWW.º£½ÇÆÆ½â°æ.COM/COMPLIANCE.