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STEMprep™ Mouse Liver Dissociation Kit

Generate viable single-cell suspensions from mouse liver tissue with the STEMprep™ Tissue Dissociation Kit and STEMprep™ Tissue Dissociator

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STEMprep™ Mouse Liver Dissociation Kit

Generate viable single-cell suspensions from mouse liver tissue with the STEMprep™ Tissue Dissociation Kit and STEMprep™ Tissue Dissociator

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Generate viable single-cell suspensions from mouse liver tissue with the STEMprep™ Tissue Dissociation Kit and STEMprep™ Tissue Dissociator
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Product Advantages

  • Achieve reproducible liver tissue dissociation when used with the STEMprep™ Tissue Dissociator
  • Ensure gentle yet effective tissue processing by combining enzymatic digestion and mechanical dissociation
  • Generate reliable single-cell suspensions from mouse liver tissue
  • Obtain high numbers of viable non-parenchymal cells, including LSECs and Kupffer cells
  • Maintain cell viability and functionality for downstream analysis

What's Included

  • STEMprep™ Mouse Liver Dissociation Kit (Catalog #100-2136)
    • STEMprep™ Enzyme A 50X, 2.5 mL
    • STEMprep™ Enzyme B 100X, 1.25 mL
    • STEMprep™ Enzyme C 50X, 2.5 mL
    • STEMprep™ Enzyme Diluent Z, 125 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. STEMprep™ Tissue Dissociator 4-sample system
    STEMprep™ Tissue Dissociator

    Automate tissue dissociation with STEMprep™ to reliably achieve high-yield, high-viability cell suspensions for your research

  2. STEMprep™ Sample Tubes
    STEMprep™ Sample Tubes

    STEMprep™ Sample Tubes enable consistent tissue dissociation with the STEMprep™ Tissue Dissociator to support high-quality single-cell preparation

Overview

Generate high-quality single-cell suspensions from mouse liver tissue efficiently and reliably using the STEMprep™ Mouse Liver Dissociation Kit. Designed to work seamlessly with the STEMprep™ Tissue Dissociator, this kit features a precisely formulated enzymatic cocktail tailored for liver tissue, combined with an optimized mechanical dissociation protocol to gently dissociate liver tissue while preserving cell viability and surface marker integrity.

The process is specifically designed to yield high numbers of non-parenchymal cells, including liver sinusoidal endothelial cells (LSECs) and Kupffer cells. By combining enzymatic digestion of the extracellular matrix with controlled mechanical dissociation, this method ensures consistent results with minimal hands-on time and user variability.

After dissociation, the cell suspension can be easily filtered and optionally treated with a debris removal step to improve sample purity. The resulting single-cell suspension is immediately suitable for downstream applications such as cell separation, culture, flow cytometry, and functional, genetic, or proteomic studies.

For best results, use this kit in conjunction with the STEMprep™ Tissue Dissociator and STEMprep™ Sample Tubes.

For more information on STEMprep™, visit the STEMprep™ overview page. Additionally, explore our instrumentation overview page or download our to learn more about available service options, including warranty coverage and additional support packages.
Subtype
Enzymatic
Species
Mouse
Application
Sample Preparation
Brand
STEMprep
Area of Interest
Cancer, Immunology, Infectious Diseases

Data Figures

STEMprep™ Mouse Liver Dissociation Kit Achieves High Cell Viability and Consistent Yield

Figure 1. STEMprep™ Mouse Liver Dissociation Kit Achieves High Cell Viability and Consistent Yield

Mouse liver tissue was processed into single-cell suspensions using the STEMprep™ Mouse Liver Dissociation Kit and the STEMprep™ Tissue Dissociator or an alternative automated system. (A) Viability of total nucleated cells. (B) Yield of viable cells per whole liver tissue (900 - 2000 mg). (C and D) Proportion and yield of CD31+ liver sinusoidal endothelial cells and F4/80+CLEC4F+ Kupffer cells. Viability, yield, and subset composition were assessed by flow cytometry. Red blood cells were lysed with ammonium chloride solution before subset analysis. Data are presented as mean ± SD (n = 15 - 37).

STEMprep™-Processed Mouse Kupffer Cells Maintain Phagocytic Function

Figure 2. STEMprep™-Processed Mouse Kupffer Cells Maintain Phagocytic Function

Mouse liver tissue was dissociated into single-cell suspensions using the STEMprep™ Mouse Liver Dissociation Kit and the STEMprep™ Tissue Dissociator, or an alternative automated system. (A) Kupffer cells were isolated from the single-cell suspensions using the EasySep™ Mouse F4/80 Positive Selection Kit. (B) The isolated F4/80+CLEC4F+ cells were incubated for 2h in the presence of pHrodo™ Green-conjugated E. coli BioParticles™ (BP) at 2 - 8°C (Cold) or 37°C. The fluorescence of phagocytosed BP was measured by flow cytometry. Data are presented as mean ± SD (n = 6).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
100-2136
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-2136
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-2136
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-2136
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-2136
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (4)

Brochure
Elevate Tissue Dissociation with STEMprep™
1:44
Video
Elevate Tissue Dissociation with STEMprep™
Streamline Your Tissue Processing Using the Automated STEMprep™ Tissue Dissociator
24:08
Webinar
Streamline Your Tissue Processing Using the Automated STEMprep™ Tissue Dissociator
Scientific Poster

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT şŁ˝ÇĆĆ˝â°ć, REFER TO WWW.şŁ˝ÇĆĆ˝â°ć.COM/COMPLIANCE.