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Figure 1. ���շ��ѱ�����™ Tissue Dissociation Workflow

The ���շ��ѱ�����™ Tissue Dissociator enables efficient processing of a wide range of tissues, including mouse brain, liver, lung, spleen, and tumors. In this workflow, tissue samples are placed into a ���շ��ѱ�����™ Sample Tube and processed using the ���շ��ѱ�����™ Tissue Dissociator in combination with the appropriate ���շ��ѱ�����™ Tissue Dissociation Kit. Depending on the selected protocol-dissociation or homogenization-the resulting output is either a single-cell suspension or a sample suitable for nucleic acid isolation. ���շ��ѱ�����™-processed cells are compatible with downstream applications such as cell separation, cell culture, flow cytometry, and other assays.

Figure 2. ���շ��ѱ�����™ Cold Temperature Hold Feature Preserves Mouse Lung and Spleen Cell Viability

Mouse lung and spleen tissues were dissociated using the ���շ��ѱ�����™ Mouse Lung and Spleen Dissociation Kits, respectively. After the protocol run was completed, the samples were held on the ���շ��ѱ�����™ Tissue Dissociator for up to 24 hours at 4°C, 22°C, and 37°C. Viability of total nucleated (A) lung and (B) spleen cells. Data are presented as mean ± SD (n = 3).

Figure 3. RNA Yields from Tissues Homogenized Using the ���շ��ѱ�����™ Tissue Dissociator Are Comparable to Other Common Homogenization Methods.

Mouse (A) brain, (B) lung, or (C) liver tissues were homogenized using the ���շ��ѱ�����™ Tissue Dissociator or an alternative automated system. RNA was subsequently extracted using the EasySep™ Total Nucleic Acid Extraction Kit (with DNase I treatment). The yield of RNA per mg of tissue was assessed using the Qubit Flex Instrument and Qubit RNA Broad Range Assay kit. Data are presented as mean ± SD (n = 8).

Figure 4. ���շ��ѱ�����™ Direct Tissue Homogenization Yields High-Quality RNA

RNA integrity number (RIN) of RNA extracted from mouse (A) brain, (B) lung, or (C) liver tissues. Tissues were homogenized using the ���շ��ѱ�����™ Tissue Dissociator or an alternative automated system. RNA was subsequently extracted from the resulting homogenates by EasySep™ Total Nucleic Acid Extraction Kit (with DNase I treatment). The quality of the extracted RNA was assessed using the 2100 Bioanalyzer Instrument and Agilent RNA 6000 Nano Kit. Data are presented as mean ± SD (n = 8).

Figure 5. ���շ��ѱ�����™ Template Protocol Information

Step 1 (Prime): A brief slow spin during pre-heat.

Steps 2 and 3 (Cut): Steps 2 and 3 are looped with a repeat count of 2. A reverse spin (-40 RPM) is applied to step 3 to ensure free movement of tissue.

Note: Reduce speed for soft tissues. Increase speed or number of loops for hard tissues.

Step 4 (Incubate): Slow spin to agitate tissue during incubation with enzyme cocktail.

Step 5 (Dissociate): High-speed spin to generate single cells.