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³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator

Automate tissue dissociation with ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ to reliably achieve high-yield, high-viability cell suspensions for your research

³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator

Automate tissue dissociation with ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ to reliably achieve high-yield, high-viability cell suspensions for your research

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Automate tissue dissociation with ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ to reliably achieve high-yield, high-viability cell suspensions for your research
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Product Advantages


  • Standardize tissue dissociation for consistent, reproducible results

  • Achieve high numbers of viable, functional single cells
  • Maintain optimal processing with temperature control from 4 to 37°C

  • Start with a 4-, 8-, or 12-sample system and scale as needed

  • Use º£½ÇÆÆ½â°æ protocols or create your own for various tissue types and needs

What's Included

  • ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator 4-Sample System (Catalog #100-2112)
    • ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator Primary Unit
    • Plastic Rack for Centrifuge Tubes
  • ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator 8-Sample System (Catalog #100-2114)
    • ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator Primary Unit
    • ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator Secondary Unit
    • Plastic Rack for Centrifuge Tubes
  • ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator 12-Sample System (Catalog #100-2115)
    • ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator Primary Unit
    • 2 x ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator Secondary Units
    • Plastic Rack for Centrifuge Tubes
  • ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator 4-Sample Expansion Unit (Catalog #100-2113)
    • ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator Secondary Unit
    • Plastic Rack for Centrifuge Tubes

What Our Scientist Says

Tissue processing doesn't need to be complex and time consuming. STEMprep™ takes the hassle out of sample prep with a sleek, customizable interface and built-in temperature control to keep your samples cool and your workflow smooth. Less time prepping, more time generating data that you trust—that’s the power of STEMprep™.

Grace Poon, PhDSenior Scientist
Grace Poon, Senior Scientist

Overview

Automate and standardize tissue dissociation with the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator, a modular system designed to generate high-yield, high-viability single-cell suspensions while preserving epitope integrity and cell functionality.

³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ simplifies protocol selection and setup with its intuitive touchscreen interface, streamlining workflows and reducing the potential for human error. The system also includes integrated temperature control, ranging from 4 to 37°C, ensuring optimal processing conditions across diverse sample types and applications and eliminating the need for external cooling or heating devices. Whether you opt to use validated º£½ÇÆÆ½â°æ protocols or customize your own, ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ offers the flexibility to adapt to different tissue types and experimental needs.

The system is designed to work seamlessly with ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Sample Tubes and tissue-specific ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Dissociation Kits, enabling efficient sample processing and consistent tissue dissociation across numerous applications.

This modular benchtop platform is scalable to match your growing workflow. It starts with a Primary Unit (which includes the touchscreen interface), and you can increase throughput by adding Secondary Units, each capable of processing up to four additional samples.

System configurations begin with a 4-sample setup that includes one Primary Unit and a Plastic Rack for Centrifuge Tubes. To process more samples, you can add Secondary Units—each one adds space for four more samples. An 8-sample system includes one Primary Unit and one Secondary Unit, while a 12-sample system includes two Secondary Units. All setups come with a Plastic Rack for Centrifuge Tubes. If you need to process more than 12 samples, you can add Expansion Units to increase capacity by four samples at a time.

For more information on ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢, visit the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ overview page. Additionally, explore our instrumentation overview page or download our to learn more about available service options, including warranty coverage and additional support packages.
Application
Sample Preparation
Brand
STEMprep
Area of Interest
Cancer, Immunology, Infectious Diseases

Data Figures

³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociation Workflow

Figure 1. ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociation Workflow

The ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator enables efficient processing of a wide range of tissues, including mouse brain, liver, lung, spleen, and tumors. In this workflow, tissue samples are placed into a ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Sample Tube and processed using the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator in combination with the appropriate ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociation Kit. Depending on the selected protocol-dissociation or homogenization-the resulting output is either a single-cell suspension or a sample suitable for nucleic acid isolation. ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢-processed cells are compatible with downstream applications such as cell separation, cell culture, flow cytometry, and other assays.

³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Cold Temperature Hold Feature Preserves Mouse Lung and Spleen Cell Viability

Figure 2. ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Cold Temperature Hold Feature Preserves Mouse Lung and Spleen Cell Viability

Mouse lung and spleen tissues were dissociated using the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Mouse Lung and Spleen Dissociation Kits, respectively. After the protocol run was completed, the samples were held on the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator for up to 24 hours at 4°C, 22°C, and 37°C. Viability of total nucleated (A) lung and (B) spleen cells. Data are presented as mean ± SD (n = 3).

RNA Yields from Tissues Homogenized Using the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator Are Comparable to Other Common Homogenization Methods

Figure 3. RNA Yields from Tissues Homogenized Using the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator Are Comparable to Other Common Homogenization Methods.

Mouse (A) brain, (B) lung, or (C) liver tissues were homogenized using the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator or an alternative automated system. RNA was subsequently extracted using the EasySepâ„¢ Total Nucleic Acid Extraction Kit (with DNase I treatment). The yield of RNA per mg of tissue was assessed using the Qubit Flex Instrument and Qubit RNA Broad Range Assay kit. Data are presented as mean ± SD (n = 8).

³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Direct Tissue Homogenization Yields High-Quality RNA

Figure 4. ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Direct Tissue Homogenization Yields High-Quality RNA

RNA integrity number (RIN) of RNA extracted from mouse (A) brain, (B) lung, or (C) liver tissues. Tissues were homogenized using the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator or an alternative automated system. RNA was subsequently extracted from the resulting homogenates by EasySepâ„¢ Total Nucleic Acid Extraction Kit (with DNase I treatment). The quality of the extracted RNA was assessed using the 2100 Bioanalyzer Instrument and Agilent RNA 6000 Nano Kit. Data are presented as mean ± SD (n = 8).

³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Template Protocol Information

Figure 5. ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Template Protocol Information

Step 1 (Prime): A brief slow spin during pre-heat.

Steps 2 and 3 (Cut): Steps 2 and 3 are looped with a repeat count of 2. A reverse spin (-40 RPM) is applied to step 3 to ensure free movement of tissue.

Note: Reduce speed for soft tissues. Increase speed or number of loops for hard tissues.

Step 4 (Incubate): Slow spin to agitate tissue during incubation with enzyme cocktail.

Step 5 (Dissociate): High-speed spin to generate single cells.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
100-2112
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-2112
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-2112
Lot #
All
Language
English