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Automated Tissue Dissociation for High-Quality Single-Cell Suspensions
Research and development in various fields, including cancer and immunology, rely on single-cell suspensions for use in applications such as flow cytometry and single-cell sequencing. Effective tissue dissociation methods are needed to generate single-cell suspensions with high viability. However, conventional manual dissociation methods can be labor-intensive, inconsistent, and prone to variability, all of which can negatively affect reproducibility and data accuracy.
To address these challenges, we developed the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociation System—an automated solution that standardizes and streamlines the dissociation process. With built-in temperature control, modular scalability, and an intuitive interface, ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ ensures efficient, reproducible, and high-yield single-cell suspensions from a wide range of solid tissues while preserving epitope integrity and cell functionality.
Why Use ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢?
Standardize tissue dissociation with automation for consistent, reproducible results.
Obtain high numbers of viable single cells with preserved functionality for downstream applications.
Ensure optimal sample processing for various sample types and applications with built-in temperature control from 4 to 37°C.
Choose from a 4-sample, 8-sample, or 12-sample system—scaling seamlessly to meet increasing throughput needs while maintaining efficiency.
Use validated º£½ÇÆÆ½â°æ protocols or create your own to adapt ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ to different tissue types and experimental needs.
³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator is an automated, modular system for generating high-yield, high-viability single-cell suspensions. It combines mechanical and enzymatic dissociation under controlled conditions, reducing user variability and manual effort. With built-in temperature control (4 - 37°C) and a user-friendly touchscreen, it ensures optimal, reproducible results. Designed to work with ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Sample Tubes and Dissociation Kits, the system scales from 4 to 12 samples with Primary and Secondary Units, and up to 16 with an optional Expansion Unit.
Advantages:
Standardize tissue dissociation for consistent, reproducible results.
Achieve high numbers of viable, functional single cells.
Maintain optimal processing with temperature control from 4 to 37°C.
Start with a 4-, 8-, or 12-sample system and scale as needed.
Use º£½ÇÆÆ½â°æ protocols or create your own for various tissue types and needs.
Size:
4-Sample System 8-Sample System 12-Sample System
Note: If you need to go beyond the 12-sample system, you can purchase an additional Expansion Unit to increase sample capacity by 4.
Each ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociation Kit includes an optimized enzymatic cocktail formulated to gently dissociate tissue while preserving cell viability and functionality. When used as part of the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ system, it streamlines workflows and produces high-quality samples suitable for downstream applications such as cell separation, culture, phenotyping, and molecular analysis.
Advantages:
Achieve reproducible tissue dissociation when used with the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator
Ensure gentle yet effective tissue processing by combining enzymatic digestion and mechanical dissociation
³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Sample Tubes are single-use tubes made for the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator and compatible kits. They enable standardized, efficient mechanical tissue dissociation across various sample types. Each tube includes a blade-integrated reservoir, central rotor, and secure lid, and is ready for immediate use. Once installed, the rotor provides controlled, reproducible dissociation—minimizing manual variability and streamlining workflows.
Advantages:
Enable reproducible tissue dissociation with the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator
Support a wide range of input sizes (20 - 4000 mg) and volumes (1 - 10 mL)
Minimize user variability for consistent downstream results
Protect sample integrity with single-use format
Size:
25 sample tubes
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Seamless Integration into Your Workflow
³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ integrates with º£½ÇÆÆ½â°æ Technologies’ tissue processing and cell isolation solutions, enabling an end-to-end workflow. Resultant cell suspensions can be used with ·¡²¹²õ²â³§±ð±èâ„¢ for column-free cell isolation; ±õ³¾³¾³Ü²Ô´Ç°ä³Ü±ô³Ùâ„¢ immune cell culture media, activators, and supplements; or other º£½ÇÆÆ½â°æ reagents to maximize cell purity and recovery.
The ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator enables efficient processing of various tissue types, including mouse brain, liver, lung, spleen, and tumors. In this workflow, a tissue is placed into the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ sample tube and processed using the ³§°Õ·¡²Ñ±è°ù±ð±èâ„¢ Tissue Dissociator. Depending on the selected protocol—dissociation or homogenization—the resulting sample will either be a single-cell suspension or nucleic acids. The processed sample can then be used for various downstream applications, such as cell isolation, activation, culture, and analysis.
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