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ܲԴǰܱ™ Immune Cell Culture

Activation, Expansion, Maintenance and Differentiation of Immune Cells

Ensure optimal activation, expansion, and differentiation of immune cell subsets using ܲԴǰܱ™ products. These immune cell culture media, activators, and supplements allow you to culture various cell types, including T cells, B cells, NK cells, dendritic cells, and macrophages, under defined stimulatory conditions for consistent and reliable results.

Why Use ܲԴǰܱ™?

  • Activate, expand, or differentiate immune cells in culture conditions optimized to promote high yield and frequency.
  • Minimize variation by using serum-free and animal-component free culture conditions.
  • Consistently achieve high yields of immune cells with the desired phenotype and function.
  • Mix and match media, activators, and supplements to suit your specific research needs.

ܲԴǰܱ™ for Activation, Expansion, and Differentiation of T Cells

T cells play a vital role in regulating the immune response, and research on these cells is essential for understanding the complex responses to various pathogens and diseases, enabling development of new treatments and cures. Advance your fundamental T cell immunology and clinical T cell engineering research with products designed to ensure optimal culture conditions for human and mouse T cells. Culture cells under defined stimulatory conditions with specialized media, activators, and supplements to activate, edit, expand, or differentiate your cell population of interest.

Learn more about our GMP-grade activation and expansion reagents for T cell therapy development and manufacturing >

GMP ܲԴǰܱ™-XF

Application:

Expansion of T Cells

Description:

High-performance, GMP-manufactured medium designed for robust expansion of human T cells without the need for serum or serum derivatives. This medium is also xeno- and phenol-red free, and has no added cytokines—providing complete flexibility for your workflows. It can be used in combination with ܲԴǰܱ™ T Cell Activators for bead-free activation of T cells.

Advantages:

  • Confidently expand T cells for use in clinical applications by using medium produced under relevant GMPs
  • Reduce variability by expanding cells in serum- and xeno-free culture conditions
  • Achieve robust T cell expansion with high viability
  • Obtain T cells able to produce cytokines upon restimulation
  • Activate T cells bead-free by combining with ܲԴǰܱ™ Human T Cell Activators

GMP ܲԴǰܱ™ Human T Cell Activators

Application:

Activation and expansion of T cells

Description:

GMP-manufactured activators designated to produce robust activation and expansion of human T cells for use in clinical applications–without the use of magnetic beads, feeder cells, or antigens. The gentle activation stimulus ensures a high viability of activated T cells, which can be further expanded in ܲԴǰܱ™-XF.

Advantages:

  • Generate robust activation and expansion of human T cells
  • Scale seamlessly from research to clinical applications with a GMP-manufactured reagent designed for translational workflows
  • Simplify protocols and reduce process complexity by eliminating magnetic beads or accessory reagents
  • Preserve cell viability and functionality with gentle stimulation

ܲԴǰܱ™-XF T Cell Expansion Medium

ܲԴǰܱ™ Human T Cell Expansion Medium

Application:

Expansion of T Cells

Description:

Serum-free and xeno-free medium optimized for the in vitro culture and expansion of mouse or human T cells isolated from peripheral blood. It can be used in combination with ܲԴǰܱ™ T Cell Activators for bead-free activation of T cells.

Advantages:

  • Eliminate the need to supplement the medium with serum
  • Achieve robust T cell expansion at levels comparable to those in serum-containing media
  • Maintain CD4⁺/CD8⁺ cell proportions comparable to the start of culture following expansion
  • Enable cytokine production in expanded T cells, including IFN-gamma and IL-4

ܲԴǰܱ™ Human T Cell Activators

Application:

Activation and expansion of T cells

Description:

Soluble antibody complexes that recognize and cross-link CD3 and co-stimulatory molecules on the surface of the T cell in the absence of magnetic beads or feeder cells. It can be used in combination with ܲԴǰܱ™-XF T Cell Expansion Medium or any other media for culturing human T cells.

Advantages:

  • Achieve robust activation and expansion of human T cells without the use of magnetic beads feeder cells, or antigen
  • Maintain high viability of activated and expanded T cells with a gentle activation stimulus
  • Rely on a highly stable, filter-sterilized soluble reagent for reproducible results

ܲԴǰܱ™
Human T Cell Differentiation Supplements

ܲԴǰܱ™ Human T Cell Differentiation Supplements

Application:

Differentiation of T helper cell subsets

Description:

Differentiation supplements containing human cytokines and blocking antibodies formulated to promote the robust activation, expansion, and differentiation of peripheral blood-derived, naïve, CD4+ human T cells into Th1, Th2 or Treg cells.

Advantages:

  • Induce human helper T cell differentiation with a formulation specifically designed for this purpose
  • Optimize performance by pairing with ܲԴǰܱ™-XF T Cell Expansion Medium
  • Support significantly higher levels of memory T cells compared to competitor differentiation kits
  • Simplify preparation with a 50X concentrate that can be added directly to the medium after thawing and mixing

ܲԴǰܱ™ Mouse T Cell Activator Kit

ܲԴǰܱ™ Mouse T Cell Activator Kit

Application:

Activation and expansion of T cells

Description:

Kit consisting of antibody complexes that bind to and cross-link CD3 and CD28 cell surface ligands, with the option to also target CD2, thereby providing the required primary and co-stimulatory signals for T cell culture and activation.

Advantages:

  • Activate mouse T cells with confidence using a single kit that allows for flexible design
  • Achieve optimal activation without the use of magnetic beads, feeder cells, or antigen
  • Maintain functional T cells with high viability by using a gentle activation stimulus
  • Rely on a highly stable, filter-sterilized, soluble reagent that is compatible with common T cell growth media

ܲԴǰܱ™
Mouse T Cell Differentiation Supplements

ܲԴǰܱ™ Mouse T Cell Differentiation Supplements

Application:

Differentiation of T helper cell subsets

Description:

Differentiation supplements containing cytokines and blocking antibodies formulated to promote the differentiation of mouse naïve CD4+ T cells into Th1, Th2 or Treg cells.

Advantages:

  • Induce Th1, Th2, or Treg cells from naïve CD4⁺ T cells isolated from the spleen of a C57BL/6 mouse using an optimized formulation
  • Simplify preparation with a 100X concentrate that can be added directly to the medium after thawing and mixing
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The ܲԴǰܱ™ Human CD3/CD28/CD2 T Cell Activator is just excellent. When combined with Dzٳٱ𳧱™ Human CD4+ T Cell Enrichment Cocktail the resulting cultures are like Mt Fuji. That's how well that combination works. The data that we generated using this activator was the highlight of our publication. We really liked this reagent.

Kazuo Suzuki, St. Vincent's Centre for Applied Medical Research


Human T cell therapy workflow

Reagents for T Cell Therapy Research

The complex process of producing human T cells for cell therapy offers opportunities to optimize for maximum yield while retaining desired end phenotype and function. Explore additional optimized products for human T cell research, including tools for T cell isolation, genome editing, and cryopreservation.

Learn More >

ܲԴǰܱ™ for NK Cell Expansion

The ability to culture and expand NK cells has become a useful tool for researching the use of NK cells for adoptive immunotherapy in cancer patients as well as understanding basic NK cell biology. Optimize your culture conditions and expand human NK cells with high yields in feeder- and serum-free culture conditions.

ܲԴǰܱ™ NK Cell Expansion Kit

Application:

Expansion of NK cells

Description:

Expand natural killer (NK) cells in serum-free conditions without the use of problematic feeder cells. Obtain high yields with a kit that includes ܲԴǰܱ™ NK Cell Base Medium, ܲԴǰܱ™ NK Cell Expansion Supplement, and ܲԴǰܱ™ NK Cell Expansion Coating Material, providing you with a complete, easy-to-use culture system.

Advantages:

  • Expand NK cells robustly with high yield and frequency
  • Improve control and consistency with feeder- and serum-free culture conditions
  • Generate functional NK cells with cytotoxic potential
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ܲԴǰܱ™ for B Cell Expansion

The ability to culture and expand B cells in vitro has become a useful tool to study human immunity, B cell biology, and vaccine and therapeutic antibody development. Culture and expand mouse or human B cells in the absence of serum, feeder cells, or specialized culture plates.

ܲԴǰܱ™ Human B Cell Expansion Supplement

Application:

Expansion of B cells

Description:

Serum-free and animal component-free supplement formulated to promote robust in vitro expansion of human peripheral blood B cells in the absence of serum, feeder cells, or specialized culture plates. The supplement contains recombinant proteins and chemically defined components that support the activation and expansion of human B cells and their maturation to plasma cells.

Advantages:

  • Achieve robust in vitro expansion of human B cells without the use of serum, feeder cells, or specialized culture plates
  • Support consistent and reliable B cell culture with a serum- and xeno-free medium

ܲԴǰܱ™ Human B Cell Expansion Kit

Application:

Expansion of B cells

Description:

ܲԴǰܱ™ Human B Cell Expansion Kit comprises ܲԴǰܱ™-XF B Cell Base Medium and ܲԴǰܱ™-ACF Human B Cell Expansion Supplement, which together ensure consistent activation and expansion of human B cells and their maturation to plasma cells. B cells can be harvested and used directly in downstream applications after just one week or less in culture.

Advantages:

  • Expand B cells in culture conditions optimized to promote high yield and frequency
  • Minimize variation by using serum-free and animal-component free culture conditions
  • Consistently achieve high yields of B cells

ܲԴǰܱ™-XF B Cell Base Medium

Application:

Expansion of B cells

Description:

Serum-free and animal component-free medium designed to support the expansion of human B cells in combination with ܲԴǰܱ™-ACF Human B Cell Expansion Supplement.

Advantages:

  • Achieve robust in vitro expansion of human B cells without the use of serum, feeder cells, or specialized culture plates
  • Support consistent and reliable B cell culture with a serum- and xeno-free medium

ܲԴǰܱ™ Mouse B Cell Expansion Kit

Application:

Expansion of B cells

Description:

Kit consisting of ܲԴǰܱ™-XF B Cell Base Medium and ܲԴǰܱ™ACF Mouse B Cell Expansion Supplement to provide optimized culture conditions for expanding B cells from mouse splenocytes with high yields. After just 6 - 9 days in culture, cells can be harvested and used directly in your downstream applications.

Advantages:

  • Achieve robust in vitro expansion of mouse B cells without the use of serum, feeder cells, or specialized culture plates
  • Simplify your workflow, with an optimized and easy-to-use culture system
  • Obtain consistent yields of expanded mouse B cells
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ܲԴǰܱ™ for Dendritic Cell Generation

Dendritic cells (DCs) are potent antigen-presenting cells and key regulators of the immune response. These cells are of great interest for research in cancer immunotherapy, vaccines, and infectious diseases. Streamline your research on DCs with products designed for robust generation of mature DCs from isolated monocytes.

ܲԴǰܱ™ Dendritic Cell Culture Kit

Application:

Culture, differentiation, and maturation of DCs

Description:

Complete kit with all the necessary components for differentiation of human monocytes into active mature dendritic cells in 7 days.

Advantages:

  • Eliminate the need to supplement with serum by using animal component-free and serum-free medium
  • Differentiate CD14+ monocytes into immature and subsequently mature DCs with an optimized formulation
  • Achieve high yields of mature DCs with the desired phenotype
  • Generate functional, mature DCs that produce the pro-inflammatory cytokine IL-12, and induce T cell proliferation

ܲԴǰܱ™ Dendritic Cell Medium

Application:

Culture, differentiation, and maturation of DCs

Description:

Serum-free and animal-component free (ACF) medium optimized for the in vitro culture and differentiation of human monocytes into dendritic cells. It can be used in combination with ܲԴǰܱ™ DC Differentiation and Maturation Supplements.

Advantages:

  • Eliminate the need to supplement the medium with serum
  • Support DC differentiation and maturation
  • Use on its own or combine with differentiation and maturation supplements

ܲԴǰܱ™ Differentiation Supplement

ܲԴǰܱ™ Dendritic Cell Differentiation Supplement

Application:

Differentiation of monocytes to immature DCs

Description:

Contains a combination of animal component-free (ACF) recombinant human cytokines formulated to support the differentiation of immature dendritic cells from human monocytes.

Advantages:

  • Rely on a defined formulation that has been standardized and optimized
  • Promote consistent differentiation of monocytes into immature dendritic cells
  • Simplify your workflow with a ready-to-use differentiation supplement that is added directly to the medium

ܲԴǰܱ™ Maturation Supplement

ܲԴǰܱ™ Dendritic Cell Maturation Supplement

Application:

Maturation of immature to mature DCs

Description:

Formulated to support the maturation of immature dendritic cells.

Advantages:

  • Induce maturation of immature dendritic cells
  • Streamline your workflow with a ready-to-use and optimized formulation
  • Obtain high yields of mature DCs
  • Generate mature DCs that produce cytokines and induce T cell proliferation
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ܲԴǰܱ™ for Macrophage Differentiation

Macrophages are cells of the innate immune system, able to respond to infections or injury through phagocytosis as well as through the immunomodulatory cytokines they produce. Macrophages such as the “classically activated” M1 macrophages and “alternatively activated” M2 macrophages are cell types of great interest due to their role in immune regulation, tissue repair, and tumor biology. Streamline your research on macrophages with culture medium designed for robust generation of M1 or M2 macrophages from isolated monocytes.

ܲԴǰܱ™
Macrophage Medium

Application:

Culture and Differentiation of Macrophages

Description:

Serum-free medium optimized for the in vitro culture and differentiation of human monocytes into macrophages. This medium can be combined with the appropriate cytokines and stimuli to differentiate M1 and M2a macrophages.

Advantages:

  • Eliminate the need to supplement with serum
  • Support robust macrophage differentiation
  • Obtain high yields of macrophages with the desired phenotype and function
  • Collect M1 or M2 macrophages in as little as 6 days
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Antibody Finder Tool

Use our Antibody Finder Tool to ensure consistent downstream cell analysis. Find antibodies that are verified to work with ܲԴǰܱ™ products in specific applications.

Find the Right Antibody for Your Applications

Don't waste valuable research time finding the appropriate antibody for your cell analysis. Use our Antibody Finder Tool to select high-quality primary and secondary antibodies verified to work with ܲԴǰܱ™ products in specific applications.

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