Immune Tolerance
Immune tolerance is critical to prevent the development of autoimmune and inflammatory diseases. Although the concept of tolerance has long been established, researchers continue to uncover previously unknown mechanisms and therapeutic applications of immune tolerance.
Below is a collection of scientific resources for your immune tolerance research.
Featured
Regulatory T Cells
Ethan Shevach and Todd Davidson. This poster provides an updated overview of the development, phenotype and functions of regulatory T cells, in particular those subsets that express the transcription factor forkhead box P3 (FOXP3).
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How to Isolate PBMCs from Whole Blood Using the SepMateâ„¢ PBMC Isolation Tubes: 15-Minute ProtocolEasy and rapid procedure for isolating peripheral blood mononuclear cells (PBMCs) from whole blood or bone marrow using the unique SepMateâ„¢ PBMC isolation tubes -
How to Prepare a Single Cell Suspension from Frozen Samples and Prevent Cell ClumpingLearn how to harvest cells from a frozen starting sample and prepare a single cell suspension prior to performing cell isolation -
How to Prepare a Single-Cell Suspension from Primary Tissue Samples (e.g. Mouse Spleen)How to harvest cells from a mouse spleen and prepare a single cell suspension prior to performing cell isolation to enable cell separation by minimizing additional cell loss and improving the labeling of target cells -
How to Count Cells Using a HemocytometerHow to prepare and count stained cell samples using a hemocytometer for both total nucleated cell counts and viable cell counts -
How to Deplete Red Blood Cells from Small Volume Samples with HetaSepâ„¢How to use an erythrocyte aggregation agent to remove red blood cells (RBCs) from small volume blood samples prior to colony-forming unit (CFU) assays -
How to Pipette Off Samples During Cell Isolation Using the EasyEightsâ„¢ EasySepâ„¢ Cell Separation MagnetDemonstrates the proper technique for pipetting off samples, to achieve optimal results when using the EasyEightsâ„¢ EasySepâ„¢ magnet to isolate cells -
Automate Cell Isolation with the RoboSepâ„¢-S Cell Separation InstrumentStreamline your cell isolations using the fully automated RoboSepâ„¢-S -
Simultaneous Cell Isolation from Multiple Samples Using the EasyEightsâ„¢ EasySepâ„¢ MagnetEasyEightsâ„¢: Faster and Easier Multiple Sample Cell Isolations -
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySepâ„¢ DirectIntroduction to the EasySepâ„¢ Direct immunomagnetic cell separation platform -
How to Isolate Cells Directly from Whole Blood Using the EasySepâ„¢ Purple/Silver MagnetsHow to isolate highly purified immune cells directly from whole blood and takes as little as 20 minutes from start to finish -
How to Isolate Cells Directly from Whole Blood - EasySepâ„¢ Direct Protocol, EasyEights MagnetHow to isolate highly purified immune cells from up to 16 whole blood samples at once with EasySepâ„¢ Direct on the EasyEights EasySepâ„¢ Magnet and the protocol takes as little as 25 minutes from start to finish -
How to Isolate Cells Directly from Whole Blood Using the Easy 50 EasySepâ„¢ Magnet: 30-Minute ProtocolHow to isolate highly purified immune cells directly from whole blood samples of up to 30 mL with and the protocol takes as little as 30 minutes from start to finish
