Immune Tolerance
Immune tolerance is critical to prevent the development of autoimmune and inflammatory diseases. Although the concept of tolerance has long been established, researchers continue to uncover previously unknown mechanisms and therapeutic applications of immune tolerance.
Below is a collection of scientific resources for your immune tolerance research.
Regulatory T Cells
Ethan Shevach and Todd Davidson. This poster provides an updated overview of the development, phenotype and functions of regulatory T cells, in particular those subsets that express the transcription factor forkhead box P3 (FOXP3).
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- How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficollâ„¢ or Lymphoprepâ„¢)This technical guide demonstrates how to isolate peripheral blood mononuclear cells from whole blood using density gradient centrifugation
- How to Pipette Off Samples During Cell Isolation Using the EasyEightsâ„¢ EasySepâ„¢ Cell Separation MagnetDemonstrates the proper technique for pipetting off samples, to achieve optimal results when using the EasyEightsâ„¢ EasySepâ„¢ magnet to isolate cells
- How SepMateâ„¢ PBMC Isolation Tubes Work: From Whole Blood to PBMCs in 15 MinutesSepMateâ„¢, an innovative tube that enables consistent and hassle-free peripheral blood mononuclear cell (PBMC) isolation, for fast and easy PBMC isolation from whole blood in just 15 minutes using density gradient centrifugation
- Working with Small Sample Volumes or Low Start Cell Numbers Using the Purple EasySepâ„¢ and EasyPlateâ„¢ EasySepâ„¢ PlatformsTechnical tip from our dedicated team of Product and Scientific Support specialists
- Using EasySepâ„¢ Positive Selection Kits for Cell DepletionThe EasySepâ„¢ system allows you to use a positive selection kit to deplete the labeled cell type, by making changes to the separation scheme of the protocol
- How to Isolate PBMCs from Whole Blood Using the SepMateâ„¢ PBMC Isolation Tubes: 15-Minute ProtocolEasy and rapid procedure for isolating peripheral blood mononuclear cells (PBMCs) from whole blood or bone marrow using the unique SepMateâ„¢ PBMC isolation tubes
- The Easy 50 EasySepâ„¢ Magnet: Portable Large-Volume Cell Separation In 25 MinutesLearn to use the portable yet powerful Easy 50 EasySepâ„¢ magnet to obtain purified cells from large volumes & a variety of sources
- How to Isolate Cells with EasySepâ„¢ Column-Free Cell Separation TechnologyIsolation of human CD4+ T cells by negative selection using EasySepâ„¢ to separate highly purified cells in as little as 8 minutes
- Automate Cell Isolation for Up to 16 Samples with the RoboSepâ„¢-16 Cell Separation InstrumentFeatures and benefits of using the fully automated cell isolation platform RoboSepâ„¢-16 which allows you to efficiently isolate cells from up to 16 samples simultaneously
- Reducing Platelet Contamination in Blood SamplesPlatelets (thrombocytes), cell fragments derived from megakaryocytes, are used in normal blood clotting, and must be handled gently to prevent activation
- Automate Cell Isolation with the RoboSepâ„¢-S Cell Separation InstrumentStreamline your cell isolations using the fully automated RoboSepâ„¢-S