Immune Tolerance
Immune tolerance is critical to prevent the development of autoimmune and inflammatory diseases. Although the concept of tolerance has long been established, researchers continue to uncover previously unknown mechanisms and therapeutic applications of immune tolerance.
Below is a collection of scientific resources for your immune tolerance research.
Featured
Regulatory T Cells
Ethan Shevach and Todd Davidson. This poster provides an updated overview of the development, phenotype and functions of regulatory T cells, in particular those subsets that express the transcription factor forkhead box P3 (FOXP3).
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Refrigerate, Freeze, or Fix Cells for Flow Cytometry or StorageFind tips and solutions for storing, freezing, or fixing cells for later analysis or long term storage -
How to Deplete Red Blood Cells from Small Volume Samples with HetaSepâ„¢How to use an erythrocyte aggregation agent to remove red blood cells (RBCs) from small volume blood samples prior to colony-forming unit (CFU) assays -
How to Prepare a Single Cell Suspension from Frozen Samples and Prevent Cell ClumpingLearn how to harvest cells from a frozen starting sample and prepare a single cell suspension prior to performing cell isolation -
Total Nucleated Cell and Viable Cell CountsAccurate cell counts are important in experiments. Ideally both the total nucleated cell (TNC) count and the viability of the cells should be assessed -
How to Isolate Nucleated Cells from Whole Blood Using HetaSepâ„¢ Erythrocyte Aggregation AgentHetaSepâ„¢ is an erythrocyte aggregation agent used to quickly separate nucleated cells from red blood cells -
Polishing the RoboSepâ„¢-S Tip HeadBasic overview on how to use and maintain your RoboSepâ„¢-S; how to polish the tip head -
How to Isolate Cells Directly from Whole Blood Using the EasySepâ„¢ Purple/Silver MagnetsHow to isolate highly purified immune cells directly from whole blood and takes as little as 20 minutes from start to finish -
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSepâ„¢ Immunodensity Cell SeparationDescribes statistical models that can be used to estimate the probability of monoclonality for limiting dilution cloning and semi-solid cloning -
Working with Small Sample Volumes or Low Start Cell Numbers Using the Purple EasySepâ„¢ and EasyPlateâ„¢ EasySepâ„¢ PlatformsTechnical tip from our dedicated team of Product and Scientific Support specialists -
How EasySepâ„¢ Magnetic Cell Separation Technology Works: Fast and Easy Cell IsolationEasySepâ„¢ technology, a fast and easy immunomagnetic cell separation technology that allows you to isolate virtually any cell types from a variety of sample sources in as little as 8 minutes -
How SepMateâ„¢ PBMC Isolation Tubes Work: From Whole Blood to PBMCs in 15 MinutesSepMateâ„¢, an innovative tube that enables consistent and hassle-free peripheral blood mononuclear cell (PBMC) isolation, for fast and easy PBMC isolation from whole blood in just 15 minutes using density gradient centrifugation -
Reducing Platelet Contamination in Blood SamplesPlatelets (thrombocytes), cell fragments derived from megakaryocytes, are used in normal blood clotting, and must be handled gently to prevent activation
