Immune Tolerance
Immune tolerance is critical to prevent the development of autoimmune and inflammatory diseases. Although the concept of tolerance has long been established, researchers continue to uncover previously unknown mechanisms and therapeutic applications of immune tolerance.
Below is a collection of scientific resources for your immune tolerance research.
Featured
Regulatory T Cells
Ethan Shevach and Todd Davidson. This poster provides an updated overview of the development, phenotype and functions of regulatory T cells, in particular those subsets that express the transcription factor forkhead box P3 (FOXP3).
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Isolating Cells from Atypical SamplesTechnical tip from our dedicated team of Product and Scientific Support specialists -
Using EasySepâ„¢ Positive Selection Kits for Cell DepletionThe EasySepâ„¢ system allows you to use a positive selection kit to deplete the labeled cell type, by making changes to the separation scheme of the protocol -
Considerations for Flow Cytometry GatingTo achieve accurate results, it is important to use an optimal gating strategy when assessing cell samples via flow cytometry -
Total Nucleated Cell and Viable Cell CountsAccurate cell counts are important in experiments. Ideally both the total nucleated cell (TNC) count and the viability of the cells should be assessed -
Refrigerate, Freeze, or Fix Cells for Flow Cytometry or StorageFind tips and solutions for storing, freezing, or fixing cells for later analysis or long term storage -
Processing Multiple Samples at One Time with the EasyEightsâ„¢ MagnetThe EasyEightsâ„¢ EasySepâ„¢ Magnet is for cell separation procedures using selected EasySepâ„¢ reagents when simultaneously processing multiple samples -
Isolate Multiple Cell Types from a Single Sample with Sequential IsolationSequential separation, for higher recovery of purified cells from limited sample size and isolation of multiple cell types, is ideal for chimerism analysis -
Assessment of Your Starting Sample and Calculation of Recovery from Cell Isolation ProceduresSetting aside a small portion of your starting sample allows you to assess the frequency of desired cells in the sample prior to isolation -
Isolate Cells with a Simple Pour-Off: EasySepâ„¢ Cell Separation TechnologyLearn how to perform the EasySepâ„¢ pour-off -
The Easy 50 EasySepâ„¢ Magnet: Portable Large-Volume Cell Separation In 25 MinutesLearn to use the portable yet powerful Easy 50 EasySepâ„¢ magnet to obtain purified cells from large volumes & a variety of sources -
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficollâ„¢ or Lymphoprepâ„¢)This technical guide demonstrates how to isolate peripheral blood mononuclear cells from whole blood using density gradient centrifugation -
How to Isolate Nucleated Cells from Whole Blood Using HetaSepâ„¢ Erythrocyte Aggregation AgentHetaSepâ„¢ is an erythrocyte aggregation agent used to quickly separate nucleated cells from red blood cells
