Immune Tolerance
Immune tolerance is critical to prevent the development of autoimmune and inflammatory diseases. Although the concept of tolerance has long been established, researchers continue to uncover previously unknown mechanisms and therapeutic applications of immune tolerance.
Below is a collection of scientific resources for your immune tolerance research.
Featured
Regulatory T Cells
Ethan Shevach and Todd Davidson. This poster provides an updated overview of the development, phenotype and functions of regulatory T cells, in particular those subsets that express the transcription factor forkhead box P3 (FOXP3).
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Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySepâ„¢ DirectIntroduction to the EasySepâ„¢ Direct immunomagnetic cell separation platform -
Preparing a Polymorphonuclear Cell Fraction from Whole Blood using Ammonium Chloride LysisTechnical tip from our dedicated team of Product and Scientific Support specialists -
Processing Multiple Samples at One Time with the EasyEightsâ„¢ MagnetThe EasyEightsâ„¢ EasySepâ„¢ Magnet is for cell separation procedures using selected EasySepâ„¢ reagents when simultaneously processing multiple samples -
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficollâ„¢ or Lymphoprepâ„¢)This technical guide demonstrates how to isolate peripheral blood mononuclear cells from whole blood using density gradient centrifugation -
How to Prepare a Single-Cell Suspension from Primary Tissue Samples (e.g. Mouse Spleen)How to harvest cells from a mouse spleen and prepare a single cell suspension prior to performing cell isolation to enable cell separation by minimizing additional cell loss and improving the labeling of target cells -
The Human Leukocyte Antigen (HLA) ComplexProvides an overview of the human leukocyte antigen (HLA) complex and nomenclature of HLA alleles -
Simultaneous Cell Isolation from Multiple Samples Using the EasyEightsâ„¢ EasySepâ„¢ MagnetEasyEightsâ„¢: Faster and Easier Multiple Sample Cell Isolations -
Using Cell Density Gradients to Optimize Your Cell IsolationsRosetteSepâ„¢, a unique immunodensity cell isolation platform, isolates highly purified cells by density gradient centrifugation without columns or magnets -
Selecting a Protocol on the RoboSepâ„¢-SBasic overview on how to use and maintain your RoboSepâ„¢-S; steps involved to select a cell separation protocol -
How to Isolate Cells with EasySepâ„¢ Column-Free Cell Separation TechnologyIsolation of human CD4+ T cells by negative selection using EasySepâ„¢ to separate highly purified cells in as little as 8 minutes -
How to Count Cells Using a HemocytometerHow to prepare and count stained cell samples using a hemocytometer for both total nucleated cell counts and viable cell counts -
Loading the RoboSepâ„¢-S Carousel and Starting a RunBasic overview on how to use and maintain your RoboSepâ„¢-S; steps to load the instrument with samples and reagents and start a cell separation run
