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Figure 1. STEMprep™ Mouse Tumor Dissociation Kit Achieves High Cell Viability and Yield

Mouse B16 tumor tissue was processed into single-cell suspensions using the STEMprep™ Mouse Tumor Dissociation Kit and the STEMprep™ Tissue Dissociator, an alternative automated system, or a manual dissociation method. (A) Viability of nucleated cells. (B) Yield of viable cells per mg of tumor tissue. (C and D) Proportion and yield of CD45+ immune and CD45- non-immune cells. Viability, yield, and subset composition were assessed by flow cytometry. Red blood cells were lysed with ammonium chloride solution before analysis. Data are presented as mean ± SD (n = 7 - 24); * p < 0.05, one-way ANOVA with Tukey's multiple comparisons test.

Figure 2. Intratumoral CD11b+ Cells Retain Immunosuppressive Function Downstream of STEMprep™ and EasySep™ Enrichment

Mouse 4T1 and B16 tumors were dissociated into single-cell suspensions using the STEMprep™ Mouse Tumor Dissociation Kit and the STEMprep™ Tissue Dissociator, followed by enrichment of CD11b+ cells. Splenic CD3+ T responder (Tresp) cells were labeled with CellTrace™ Violet, activated with the ImmunoCult™ Mouse T Cell CD3/CD28 soluble activator, and cultured either alone or with CD11b-enriched cells at increasing CD11b:Tresp cell ratios at 37°C for 4 days. (A) Purity of CD11b+ cells before and after enrichment with the EasySep™ Mouse CD11b Positive Selection Kit II. (B) Representative histograms of Tresp proliferation in the presence or absence of CD11b enriched cells. (C) Summary of Tresp suppression by CD11b enriched cells from 4T1 and B16 tumors. Data are presented as the mean of 2 independent experiments.