Directed Differentiation and Disease Modeling
Human pluripotent stem cell (hPSC)-based models hold tremendous potential for studying human development and disease. Directed differentiation and disease modeling are two key methods for using hPSCs in drug discovery, cell therapy validation, and disease research.
- Directed differentiation of hPSCs refers to the in vitro differentiation of these cells toward a specific cell type through defined cell culture conditions. Directed differentiation is achieved by the addition of specific growth factors or small molecules.
- Disease modeling is an approach to study diseases using cells that display relevant pathological features. Disease modeling using hPSCs can be achieved by reproduction of a disorder-associated mutation with gene editing, isolation of embryonic stem cells (ESCs) from affected blastocysts; or generation of induced pluripotent stem cells (iPSCs) from patients’ somatic cells.
Explore the resources below to support your disease modeling studies using hPSCs.
Highly Efficient Single-Cell Human Pluripotent Stem Cell Cloning and Robust Cardiomyocyte Differentiation
Recorded during the ISSCR 2017 Innovation Showcase in Boston, this tutorial highlights human pluripotent stem cell (hPSC) gene-editing and cardiac differentiation workflows using the CloneRâ„¢ supplement and the STEMdiffâ„¢ Cardiomyocyte System. This talk is presented º£½ÇÆƽâ°æ’ Dr. Adam Hirst, scientist for pluripotent stem cell biology, and Dr. Vincenzo Macri, senior scientist for cardiomyocyte stem cell biology.
View Now >-
STEMdiffâ„¢ Kits for Robust and Efficient Differentiation of hPSCs to Multiple Cell TypesDr. Melanie Kardel, scientist at º£½ÇÆƽâ°æ Technologies, describes the use of STEMdiffâ„¢ kits to differentiate human pluripotent stem cells to all three germ layers. This video was filmed during the Innovation Showcase at ISSCR 2016 in San Francisco. -
Cerebral Organoids as 3D, Stem Cell-Derived Models of Tuberous Sclerosis ComplexIn this webinar, Dr. Juergen Knoblich presents data from his preprint, "Cerebral organoid model reveals excessive proliferation of human caudal late interneuron progenitors in Tuberous Sclerosis Complex". -
Modeling Alzheimer's risk using human TREM2-knockout microgliaAmanda McQuade from Dr. Mathew Blurton-Jones’s lab discusses her protocol for differentiating microglia from induced pluripotent stem cells (iPSCs) and the use of these microglia in vivo and in vitro to uncover the mechanisms of immune activation and neurodegeneration in Alzheimer’s disease.
