Directed Differentiation and Disease Modeling
Human pluripotent stem cell (hPSC)-based models hold tremendous potential for studying human development and disease. Directed differentiation and disease modeling are two key methods for using hPSCs in drug discovery, cell therapy validation, and disease research.
- Directed differentiation of hPSCs refers to the in vitro differentiation of these cells toward a specific cell type through defined cell culture conditions. Directed differentiation is achieved by the addition of specific growth factors or small molecules.
- Disease modeling is an approach to study diseases using cells that display relevant pathological features. Disease modeling using hPSCs can be achieved by reproduction of a disorder-associated mutation with gene editing, isolation of embryonic stem cells (ESCs) from affected blastocysts; or generation of induced pluripotent stem cells (iPSCs) from patients’ somatic cells.
Explore the resources below to support your disease modeling studies using hPSCs.
Highly Efficient Single-Cell Human Pluripotent Stem Cell Cloning and Robust Cardiomyocyte Differentiation
Recorded during the ISSCR 2017 Innovation Showcase in Boston, this tutorial highlights human pluripotent stem cell (hPSC) gene-editing and cardiac differentiation workflows using the CloneRâ„¢ supplement and the STEMdiffâ„¢ Cardiomyocyte System. This talk is presented º£½ÇÆƽâ°æ’ Dr. Adam Hirst, scientist for pluripotent stem cell biology, and Dr. Vincenzo Macri, senior scientist for cardiomyocyte stem cell biology.
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iPSCs As Models, Part 1: What Are Induced Pluripotent Stem Cells and How Do You Use Them?Hear from Dr. Anjana Nityanandam, Director of the Human Stem Cell Lab core facility at St. Jude Children’s Research Hospital, as she provides some introductory examples of how iPSCs are used for disease modeling and walks through how to generate and characterize iPSCs. Learn about quality control measures to improve reliability and reproducibility of data, and how to address interline variability in your experiments. -
iPSCs As Models, Part 2: Modeling the Human Brain with OrganoidsGet a walkthrough of how to generate and characterize iPSC-derived brain organoids from Dr. Anjana Nityanandam, Director of the Human Stem Cell Lab core facility at St. Jude Children’s Research Hospital. Learn how to improve reliability and reproducibility of data from brain organoid models, and get an overview of disease modeling case studies using various 3D cultures. -
Derivation and Applications of Human Pluripotent Stem CellsOverview of the derivation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) -
Building Three-Dimensional Human Brain OrganoidsOverview of brain organogenesis and the applications of brain organoids in studying the development and maturation of the nervous system
