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EasySep? Release Human CD4 Positive Selection Kit

Immunomagnetic positive selection of human CD4+ T cells from PBMCs or washed leukapheresis samples using particle release technology

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep? Release Human CD4 Positive Selection Kit

Immunomagnetic positive selection of human CD4+ T cells from PBMCs or washed leukapheresis samples using particle release technology

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Immunomagnetic positive selection of human CD4+ T cells from PBMCs or washed leukapheresis samples using particle release technology
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Product Advantages


  • Highly purified human CD4+ cells isolated in less than 30 minutes

  • No-wash removal of EasySep? Releasable RapidSpheres?

What's Included

  • EasySep? Release Human CD4 Positive Selection Kit (Catalog #17752)
    • EasySep? Release Human CD4 Positive Selection Cocktail, 1 mL
    • EasySep? Releasable RapidSpheres? 50201, 1 mL
    • EasySep? Release Buffer (Concentrate), 3 x 1 mL
  • RoboSep? Human CD4 Positive Selection Kit (Catalog #17752RF)
    • EasySep? Release Human CD4 Positive Selection Cocktail, 1 mL
    • EasySep? Releasable RapidSpheres?, 1 mL
    • EasySep? Release Buffer (Concentrate), 3 x 1 mL
    • RoboSep? Buffer (Catalog #20104)
    • RoboSep? Filter Tips (Catalog #20125)
    • EasySep? EasyTube?-14 (Catalog #20128)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer for use with EasySep? cell separation kits

Overview

Easily isolate highly purified and magnetic particle-free human CD4+ cells from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples, using immunomagnetic positive selection, with the EasySep? Release Human CD4 Positive Selection Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

This EasySep? positive selection procedure involves labeling CD4+ cells with antibody complexes and EasySep? Releasable RapidSpheres?. Unlike traditional magnetic particles that stay bound to the target cells, RapidSpheres? have a releasable feature. Desired cells are first labeled with antibodies and these specialized magnetic particles, and separated without columns using an EasySep? magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep?-isolated, CD4+ cells using a release agent. Following magnetic cell isolation in as little as 30 minutes with this EasySep? Release kit, the desired cells are immediately available for downstream applications, such as flow cytometry, culture, or DNA/RNA extraction. Antibody complexes remain bound to the surface of the desired cells and may interact with Brilliant Violet? antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands. The CD4 antigen is strongly expressed on helper T cells and is expressed more weakly on peripheral blood monocytes and tissue macrophages.

Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep?. Explore additional products optimized for your workflow, including those for culture media, supplements, antibodies, and more.
Magnet Compatibility
? EasySep? Magnet (Catalog #18000)
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? EasyPlate? Magnet (Catalog #18102)
? EasyEights? Magnet (Catalog #18103)
? RoboSep?-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD4+
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Brand
EasySep
Area of Interest
Immunology, Cell Therapy Development

Data Figures

Starting with a single-cell suspension of human PBMCs, the CD4+ T cell content of the isolated fraction is typically 96.1 ± 4.1% (mean ± SD using the purple EasySep? Magnet). In the above example, the purities of the start and final isolated fractions are 24.0% and 98.1%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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17752
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17752RF
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17752RF
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17752RF
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17752RF
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17752
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17752
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17752
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All
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English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (11)

Brochure
Brochure
Brochure
Production of Chimeric Antigen Receptor T Cells
Wallchart
Production of Chimeric Antigen Receptor T Cells
The Immune Response to HIV Poster
Wallchart
The Immune Response to HIV Poster
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights? EasySep? Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights? EasySep? Magnet
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
Scientific Poster

Publications (1)

Regulatory Programs of B-cell Activation and Germinal Center Reaction Allow B-ALL Escape from CD19 CAR T-cell Therapy. N. G. Im et al. Cancer immunology research 2022 sep

Abstract

Chimeric antigen receptor (CAR) T-cell therapy has led to tremendous successes in the treatment of B-cell malignancies. However, a large fraction of treated patients relapse, often with disease expressing reduced levels of the target antigen. Here, we report that exposing CD19+ B-cell acute lymphoblastic leukemia (B-ALL) cells to CD19 CAR T cells reduced CD19 expression within hours. Initially, CD19 CAR T cells caused clustering of CD19 at the T cell-leukemia cell interface followed by CD19 internalization and decreased CD19 surface expression on the B-ALL cells. CD19 expression was then repressed by transcriptional rewiring. Using single-cell RNA sequencing and single-cell assay for transposase-accessible chromatin using sequencing, we demonstrated that a subset of refractory CD19low cells sustained decreased CD19 expression through transcriptional programs of physiologic B-cell activation and germinal center reaction. Inhibiting B-cell activation programs with the Bruton's tyrosine kinase inhibitor ibrutinib increased the cytotoxicity of CD19 CAR T cells without affecting CAR T-cell viability. These results demonstrate transcriptional plasticity as an underlying mechanism of escape from CAR T cells and highlight the importance of combining CAR T-cell therapy with targeted therapies that aim to overcome this plasticity. See related Spotlight by Zhao and Melenhorst, p. 1040.
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