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Human Peripheral Blood Mononuclear Cells, Frozen

Primary human cells, frozen

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Maximize your savings on high-quality PBMCs! Take advantage of our bulk pricing discounts and price matching offer to get the best value for your purchase. Now available for specific lots, the standard flow cytometry panel provides the exact frequencies of common cell types in each vial of PBMCs. See Figure 1 in the Data Figures section below to view a representative panel. Browse our Frequently Asked Questions for more information.

Human Peripheral Blood Mononuclear Cells, Frozen

Primary human cells, frozen

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Primary human cells, frozen
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Product Advantages

  • Get large numbers of high-quality PBMCs from a single-donor
  • Choose from a range of sizes to fit your research needs
  • Access a large donor pool inventory to test across broader populations 
  • Meet specific requirements with the options of custom products or collections
  • Hold large batches of cryopreserved cells while you test donors in your translational research 

Overview

Streamline your assays with ready-to-use, ethically sourced, primary human mononuclear cells. With personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications, we help you get the cells you need.

Isolated from peripheral blood leukapheresis samples using density gradient separation and/or red blood cell lysis and cryopreserved in animal component-free CryoStor庐CS10 medium (Catalog #07930), cells are collected using ethically approved protocols, with Institutional Review Board (IRB) approval and in compliance with applicable legislation and guidance from the U.S. Food and Drug Administration (FDA) in the United States, or with Research Ethics Committee (REC) approval and in compliance with applicable legislation and guidance from the Human Tissue Authority (HTA) in the United Kingdom. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added during collection as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above, after selecting from the product options. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.

Certain products are only available in select territories. Please contact your local sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.

Browse our Frequently Asked Questions (FAQs) on Primary Cells.
Contains
鈥 CryoStor庐 CS10
Subtype
Frozen
Cell Type
Mononuclear Cells
Species
Human
Cell and Tissue Source
Peripheral Blood
Area of Interest
Drug Discovery and Toxicity Testing
Donor Status
Normal

Data Figures

Typical Flow Cytometric Analysis Profile of PBMCs

Figure 1. Typical Flow Cytometric Analysis Profile of PBMCs

Representative gating strategy of immune cell populations present in PBMCs. Flow cytometry was performed on the peripheral blood mononuclear cells (PBMCs) post-thaw and can be provided for specific lots. The CD45 plot was gated on viable single cells while all other plots were gated on viable CD45+ single cells. In the above example, the cell frequencies are as follows: leukocytes (CD45+), 99.8%; B cells (CD19+), 10.5%; T Cells (CD3+), 57.2%; helper T cells (CD3+CD4+), 34.4%; Cytotoxic T cells (CD3+CD8+), 19.6%; monocytes (CD14+), 17.9%; and NK cells (CD3-CD56+), 7.91%.

Typical Flow Cytometric Analysis Profile of PBMCs

Figure 2. Mean Percentages of Cell Subpopulations in Cryopreserved PBMCs

Representative chart showing the average frequencies of major immune subsets in 海角破解版鈥檚 cryopreserved PBMC products, as measured by flow cytometry post-thaw. Values shown are mean percentages of total viable leukocytes present in PBMCs (n 鈮 183).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
70025.1, 70025.2, 70025.3, 70025
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (11)

Brochure
Brochure
How to Process a Leukopak for Downstream Cell Isolation
13:43
Video
How to Process a Leukopak for Downstream Cell Isolation
Evaluating the Immuno-Oncology Potential of Compounds Using Human In Vitro TME Models
43:57:00
Video
Evaluating the Immuno-Oncology Potential of Compounds Using Human In Vitro TME Models
How to Thaw Frozen Human Primary Cells
6:05
Video
How to Thaw Frozen Human Primary Cells
How to Quickly Thaw Frozen Cells with the ThawStar® CFT2 Automated Thawing System
1:49
Video
How to Quickly Thaw Frozen Cells with the ThawStar® CFT2 Automated Thawing System
New Guidelines for T Cell Nomenclature
1:01:42
Webinar
New Guidelines for T Cell Nomenclature
Leukopak Processing: Tips & Tricks for Streamlined Cell Isolation
1:01:09
Webinar
Leukopak Processing: Tips & Tricks for Streamlined Cell Isolation
How to Use Primary Cells to Develop the Next Therapy
45:38
Webinar
How to Use Primary Cells to Develop the Next Therapy
Human Immune Cell Isolation Course
On-Demand Training
Human Immune Cell Isolation Course
Application of HSPCs in Cell Therapy Course
On-Demand Training
Application of HSPCs in Cell Therapy Course

Frequently Asked Questions

What is the expected viability of frozen PBMCs?

Most frozen primary cell products have the pre-cryopreservation viability and the accepted viability upon thawing reported on the Certificate of Analysis (CoA). Provided that proper thawing technique is used and viability is assessed immediately upon thaw, the product should meet this specification. PBMCs have an acceptance criterion of 鈮 90% viable cells.
If you have questions regarding our specifications and/or concerns about the viability of the product you received, please contact your sales representative or Product and Scientific Support (techsupport@stemcell.com).
For a step-by-step protocol for thawing frozen primary cells, watch our protocol video.

Do you provide temperature-controlled shipping and data logging for frozen PBMC shipments?

Our standard shipping conditions for frozen PBMCs consists of styrofoam-insulated shipping boxes with sufficient dry ice to maintain product quality for the duration of the shipment. We can also provide temperature data loggers or use LN2 shippers for an added fee upon request. Contact your sales representative or cellorders@stemcell.com for further details.

What is the best procedure to maximize viability after thawing?

Proper thawing and handling of these cryopreserved cells is critical for optimal viability and recovery. As thawing protocols may vary by cell type, always refer to the recommended protocol received with your cells. You can also view this protocol video on thawing frozen human primary cells.

What type of cryovials do frozen PBMCs arrive in?

Our frozen PBMCs are cryopreserved in 2 mL polypropylene barcoded cryovials, (Corning Catalog #8670, internal-threaded). If there are supply issues, we may use Corning Catalog #8671 (external-threaded).
Some older inventory may be cryopreserved in 2 mL non-barcoded cryovials (Corning Catalog #430488).
Vials are barcoded on the bottom for customization and traceability. They are compatible with various e-lab software and biostore units, including Benchling, Labguru, and SciNote.

How are PBMCs validated?

海角破解版鈥檚 primary cells are validated using our Quality Management System, which assesses cell count, viability, and purity. 海角破解版's Quality Management System is certified to ISO 13485:2016 Medical Devices and ISO 9001:2015.
Learn more: Partner with the Right Human Primary Cell Supplier

Are our PBMCs serum-free?

海角破解版's fresh and frozen PBMCs are not considered serum-free by 海角破解版's definition. Cryopreserved PBMCs are shipped in CryoStor庐 CS10 cryopreservation medium (Catalog #100-1061), which is serum-free, but the preparation of this sample may leave trace amounts of serum. Fresh PBMCs are supplied in Iscove鈥檚 Modified Dulbecco鈥檚 Medium (Catalog #36150) with 10% fetal bovine serum (FBS).
If you require further information, please contact your sales representatives or complete this form to contact the Primary Cells team directly.

Publications (13)

Unveiling the Immunomodulatory and regenerative potential of iPSC-derived mesenchymal stromal cells and their extracellular vesicles J. C. Buitrago et al. Scientific Reports 2024 Oct

Abstract

Induced pluripotent stem cell (iPSC)-derived mesenchymal stromal cells (iMSCs) offer a promising alternative to primary mesenchymal stromal cells (MSCs) and their derivatives, particularly extracellular vesicles (EVs), for use in advanced therapy medicinal products. In this study we evaluated the immunomodulatory and regenerative potential of iMSCs as well as iMSC-EVs, alongside primary human umbilical cord-derived mesenchymal stromal cells (hUCMSCs). Our findings demonstrate that iMSCs exhibit comparable abilities to hUCMSCs in regulating lymphocyte proliferation and inducing an anti-inflammatory phenotype in monocytes. We also observed decreased TNF伪 levels and increased IL-10 induction, indicating a potential mechanism for their immunomodulatory effects. Furthermore, iMSC-EVs also showed effective immunomodulation by inhibiting T cell proliferation and inducing macrophage polarization similar to their parental cells. Additionally, iMSC-EVs exhibited pro-regenerative potential akin to hUCMSC-EVs in in vitro scratch assays. Notably, priming iMSCs with pro-inflammatory cytokines significantly enhanced the immunomodulatory potential of iMSC-EVs. These results underscore the considerable promise of iMSCs and iMSCs-EVs as an alternate source for MSC-derived therapeutics, given their potent immunomodulatory and regenerative properties. The online version contains supplementary material available at 10.1038/s41598-024-75956-3.
Eradication of Triple-Negative Breast Cancer Cells by Targeting Glycosylated PD-L1. C.-W. Li et al. Cancer cell 2018 FEB

Abstract

Protein glycosylation provides proteomic diversity in regulating protein localization, stability, and activity; it remains largely unknown whether the sugar moiety contributes to immunosuppression. In the study of immune receptor glycosylation, we showed that EGF induces programmed death ligand 1 (PD-L1) and receptor programmed cell death protein 1 (PD-1) interaction, requiring beta$-1,3-N-acetylglucosaminyl transferase (B3GNT3) expression in triple-negative breast cancer. Downregulation of B3GNT3 enhances cytotoxic T cell-mediated anti-tumor immunity. A monoclonal antibody targeting glycosylated PD-L1 (gPD-L1) blocks PD-L1/PD-1 interaction and promotes PD-L1 internalization and degradation. In addition to immune reactivation, drug-conjugated gPD-L1 antibody induces a potent cell-killing effect as well as a bystander-killing effect on adjacent cancer cells lacking PD-L1 expression without any detectable toxicity. Our work suggests targeting protein glycosylation as a potential strategy to enhance immune checkpoint therapy.
Dendritic Cells but Not Macrophages Sense Tumor Mitochondrial DNA for Cross-priming through Signal Regulatory Protein α Signaling. Xu MM et al. Immunity 2017 AUG

Abstract

Inhibition of cytosolic DNA sensing represents a strategy that tumor cells use for immune evasion, but the underlying mechanisms are unclear. Here we have shown that CD47-signal regulatory protein α (SIRPα) axis dictates the fate of ingested DNA in DCs for immune evasion. Although macrophages were more potent in uptaking tumor DNA, increase of DNA sensing by blocking the interaction of SIRPα with CD47 preferentially occurred in dendritic cells (DCs) but not in macrophages. Mechanistically, CD47 blockade enabled the activation of NADPH oxidase NOX2 in DCs, which in turn inhibited phagosomal acidification and reduced the degradation of tumor mitochondrial DNA (mtDNA) in DCs. mtDNA was recognized by cyclic-GMP-AMP synthase (cGAS) in the DC cytosol, contributing to type I interferon (IFN) production and antitumor adaptive immunity. Thus, our findings have demonstrated how tumor cells inhibit innate sensing in DCs and suggested that the CD47-SIRPα axis is critical for DC-driven antitumor immunity.
View All Publications

Related Products

Related Products
Maximize your savings on high-quality PBMCs! Take advantage of our bulk pricing discounts and price matching offer to get the best value for your purchase. Now available for specific lots, the standard flow cytometry panel provides the exact frequencies of common cell types in each vial of PBMCs. See Figure 1 in the Data Figures section below to view a representative panel. Browse our Frequently Asked Questions for more information.
Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.

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