°Õ±ð³§¸éâ„¢ Pluripotent Stem Cell Culture Media
°Õ±ð³§¸éâ„¢ Pluripotent Stem Cell Culture Media
High-Quality Media for Every hPSC Workflow
The °Õ±ð³§¸éâ„¢ family of feeder-free maintenance media offers exceptional versatility for your chosen human pluripotent stem cell (hPSC) workflow, from iPSC generation and routine maintenance to gene editing, differentiation, and large-scale expansion. There are seven different °Õ±ð³§¸éâ„¢ formulations, each one optimized to support specific culture conditions. Whether you want to maintain your hPSCs as aggregates or single cells in 2D, or scale them up in 3D suspension, there is a °Õ±ð³§¸éâ„¢ medium optimized to support genetic stability and cell quality for your preferred culture system. Built on the published formulations1-3 from the laboratory of Dr. James Thomson, °Õ±ð³§¸éâ„¢ media are produced using rigorously pre-screened materials for reliable batch-to-batch consistency and reproducibility. This combination of flexibility and quality assurance allows you to seamlessly integrate °Õ±ð³§¸éâ„¢ media into your disease modeling and translational or cell therapy development workflows.
°Õ±ð³§¸éâ„¢ Product Finder
Find the right hPSC culture medium for your specific needs with this interactive tool.
Aggregate 2D Monolayer Culture
hPSCs are commonly maintained in 2D monolayer culture as multicellular aggregates or colonies, a widely used approach for preserving pluripotency and supporting routine cell expansion. The table below compares our °Õ±ð³§¸éâ„¢ media for 2D aggregate culture, highlighting key formulation features, feeding strategies, regulatory support, and recommended applications to help you identify the optimal medium for your stem cell culture workflow.
m°Õ±ð³§¸éâ„¢ Plus
Best For:
Formulation:
- Serum-Free
- Stabilized FGF2
- Enhanced pH Buffering
Regulatory Documentation:
Feeding Schedule:
Optimized Culture System:
- Routine long-term 2D aggregate/clump passaging
- Short-term single-cell passaging prior to downstream assays
Manuals, Protocols, and Tech Tips:
°Õ±ð³§¸éâ„¢-AOF
Best For:
Formulation:
- Animal Origin-Free
- Recombinant Protein-Containing
- Stabilized FGF2
- Enhanced pH Buffering
Regulatory Documentation:
Feeding Schedule:
Optimized Culture System:
- Routine long-term 2D aggregate/clump passaging
- Short-term single-cell passaging prior to downstream assays
Manuals, Protocols, and Tech Tips:
m°Õ±ð³§¸éâ„¢1
Best For:
Formulation:
- Serum-Free
Regulatory Documentation:
Feeding Schedule:
Optimized Culture System:
- Routine long-term 2D aggregate/clump passaging
- Short-term single-cell passaging prior to downstream assays
Manuals, Protocols, and Tech Tips:
°Õ±ð³§¸éâ„¢-E8
Best For:
Formulation:
- Low-Protein
- Animal Component-Free
- Xeno-Free
Regulatory Documentation:
Feeding Schedule:
Optimized Culture System:
- Routine long-term 2D aggregate/clump passaging
- Short-term single-cell passaging prior to downstream assays
Manuals, Protocols, and Tech Tips:
Single-Cell 2D Monolayer Culture
hPSCs can be maintained as single cells in 2D monolayer culture, but this approach requires a medium specifically formulated to mitigate the cellular stress associated with single-cell passaging. The table below summarizes the key features, culture conditions, and recommended uses of e°Õ±ð³§¸éâ„¢, the only medium designed to support long-term single-cell maintenance while preserving hPSC quality and genetic stability.
e°Õ±ð³§¸éâ„¢
Best For:
Formulation:
- Feeder-Free
- Serum-Free
- BSA-Containing
- Stabilized FGF2
- Enhanced pH Buffering
Regulatory Documentation:
Feeding Schedule:
Optimized Culture System:
- Routine long-term 2D single-cell passaging
- Short-term single-cell passaging prior to downstream assays
Manuals, Protocols, and Tech Tips:
Fed-Batch 3D Suspension Culture
3D suspension culture enables scalable expansion of hPSCs as aggregates in suspension, making it well suited for applications requiring increased cell yields. The table below compares our °Õ±ð³§¸éâ„¢ media for fed-batch 3D culture, outlining formulation characteristics, feeding strategies, and recommended uses to help you select the appropriate medium for your scale-up workflow.
°Õ±ð³§¸éâ„¢-AOF 3D
Best For:
Formulation:
Regulatory Documentation:
Feeding Schedule:
Optimized Culture System:
Manuals, Protocols, and Tech Tips:
m°Õ±ð³§¸éâ„¢3D
Best For:
Formulation:
Regulatory Documentation:
Feeding Schedule:
Optimized Culture System:
Manuals, Protocols, and Tech Tips:
hPSC Sourcing & Reprogramming
°Õ±ð³§¸éâ„¢ media are compatible with multiple starting points, whether you prefer to purchase our well-characterized, high-quality iPSCs or to generate your own using our trusted reprogramming media and technologies. iPSCs offer a versatile alternative to embryonic stem cells, enabling researchers to create patient-specific pluripotent cell lines from adult tissues for disease modeling, drug discovery, gene editing, and regenerative medicine applications. From ready-to-use cell lines to end-to-end solutions for reprogramming primary samples, working with °Õ±ð³§¸éâ„¢ gives you the flexibility to build your preferred workflow.
hPSC Differentiation
°Õ±ð³§¸éâ„¢ is designed to work seamlessly with ³§°Õ·¡²Ñ»å¾±´Ú´Úâ„¢ differentiation media, enabling smooth transitions from pluripotent maintenance to directed differentiation. Whether you’re generating definitive germ layers, specialized cell types, or 3D organoids, this compatibility helps streamline workflows and support consistent, reproducible outcomes.
Accessory Products
Robust hPSC workflows depend on more than maintenance media alone. °Õ±ð³§¸éâ„¢ is supported by a comprehensive suite of workflow reagents, including matrices, passaging reagents, cryopreservation reagents, and survival supplements to help protect cell health at every step. Together, these reagents are designed to streamline routine culture, enhance cell survival, and support consistent expansion and recovery of human pluripotent stem cells.
Resources & Training for hPSC Culture
Success with human pluripotent stem cell culture starts with operator skill and experience. °Õ±ð³§¸éâ„¢ is backed by on-demand training, practical educational resources, and access to º£½ÇÆƽâ°æ’s dedicated Product and Scientific Support team to help you troubleshoot, optimize workflows, and apply best practices with confidence. Together, these resources are designed to support both new and experienced users at every stage of their pluripotent stem cell research.
