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CellPoreā„¢ FITC-Dextran

Fluorescent tracer for visualizing and optimizing intracellular delivery efficiency with CellPoreā„¢ Transfection System

CellPoreā„¢ FITC-Dextran

Fluorescent tracer for visualizing and optimizing intracellular delivery efficiency with CellPoreā„¢ Transfection System

From: 51 CAD
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Fluorescent tracer for visualizing and optimizing intracellular delivery efficiency with CellPoreā„¢ Transfection System

Product Advantages

  • Optimize mechanoporation efficiency with a ready-to-use positive control cargo 
  • Quantify intracellular delivery by flow cytometry to optimize pressure while maintaining cell viability
  • Maintain cell health and data quality with minimal toxicity and low immunogenicity
  • Co-deliver with functional cargo and use in multicolor panels to monitor delivery efficiency in complex assays
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

Optimize mechanoporation-based delivery with the CellPoreā„¢ Transfection System by using CellPoreā„¢ FITC-Dextran, a green fluorescent tracer designed to assess transfection efficiency. CellPoreā„¢ FITC-Dextran comes in a ready-to-use format, making it an easy-to-use universal positive control cargo for all your applications.

This hydrophilic polysaccharide, consisting of dextran labeled with fluorescein isothiocyanate (FITC), is characterized by minimal toxicity and low immunogenicity, enabling reliable downstream analysis with minimal impact on cell health.

During mechanoporation, transient membrane permeabilization enables rapid FITC-dextran entry, resulting in markedly stronger intracellular fluorescence compared to natural uptake by endocytosis. Use CellPoreā„¢ FITC-Dextran as a positive control to determine the optimal CellPoreā„¢ delivery pressure for your cell type and application. Following your initial optimization run, select the pressure that maximizes uptake while preserving viability for subsequent experiments. CellPoreā„¢ FITC-Dextran can also be co-delivered with other functional cargo to track transfection efficiency in parallel with functional assays. Delivery performance can be analyzed by flow cytometry, and the marker is compatible with multi-color staining panels.

For best results, use in conjunction with the CellPoreā„¢ Transfection System, CellPoreā„¢ Delivery Cartridges, and CellPoreā„¢ Delivery Media.

For more information about CellPoreā„¢, including the intracellular delivery technology used in the CellPoreā„¢ Transfection System, visit the CellPoreā„¢ overview page.
Cell Type
Hematopoietic Stem and Progenitor Cells, Monocytes, NK Cells, Pluripotent Stem Cells, T Cells, T Cells, CD4+, T Cells, CD8+
Species
Human, Mouse
Application
Genome Editing
Brand
CellPore
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Immunology

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
100-1024
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-1024
Lot #
All
Language
English