ʴǰ™
ʴǰ™ Transfection System
Gentle Intracellular Delivery with High Efficiency and Viability
Intracellular delivery of molecules is crucial for advancing biological research and developing innovative cell and gene-based therapies. Traditional methods for transfecting cells are often limited in their ability to deliver a wide range of cargoes across diverse cell types. Additionally, these methods can inadvertently alter gene expression and disrupt cell function.
To address your cell engineering needs, we developed the ʴǰ™ Transfection System—an instrument offering versatility in delivering a variety of macromolecules into the cytosol of cells. Using gentle microfluidic technology, the system provides a simple, intuitive workflow that can be tailored to your specific research applications.
How Does ʴǰ™ Work?
The system comprises a benchtop instrument, specialized ʴǰ™ Delivery Cartridges, and ʴǰ™ Delivery Media. Using proprietary Cell Squeeze™ mechanoporation technology, the ʴǰ™ Transfection system uses compressed air to rapidly deform cells through microfluidic channels, to create transient membrane pores, allowing target cargoes to enter the cytosol before the membrane reseals.
Why Use ʴǰ™?
- Achieve high delivery and viability for your cells
- Fine-tune a single parameter to identify optimal delivery and viability conditions
- Deliver cargoes intracellularly without altering cell quality
- Transfect up to 10 million cells per second
- Deliver a wide variety of cargoes to a broad range of cell types
Figure 1. ʴǰ™ Transfection System Workflow
This figure illustrates the ʴǰ™ Transfection System workflow in three straightforward steps: 1) prepare cells sourced from freshly isolated or frozen samples using cell isolation technology such as ⳧™; 2) deliver one or more desired cargoes into your cells using the ʴǰ™ Transfection System; 3) analyze the delivery efficiency for downstream applications.
ʴǰ™ Products
ʴǰ™ Transfection System
Description:
The ʴǰ™ Transfection System is a microfluidic platform designed for the gentle, high-efficiency intracellular delivery of diverse cargoes. Using proprietary Cell Squeeze™ mechanoporation technology, the system uses compressed air to rapidly deform cells through microfluidic channels, transiently permeabilizing the membrane while preserving cell viability.
Advantages:
- Achieve high delivery and viability for your cells
- Fine-tune a single parameter to identify optimal delivery and viability conditions
- Deliver cargoes intracellularly without altering cell quality
- Transfect up to 10 million cells per second
- Deliver a wide variety of cargoes to a broad range of cell types
ʴǰ™ Delivery Cartridges
Description:
ʴǰ™ Delivery Cartridges are single-use pre-assembled cartridges featuring precision-engineered microchannels that leverage mechanical deformation to gently permeabilize cell membranes. These delivery cartridges enable the efficient cytosolic delivery of diverse cargoes into sensitive cell types, including immune cells and pluripotent stem cells, while preserving cell viability and function.
Advantages:
- Maintain delivery efficiency and viability with single-use cartridges for a range of immune, stem, and engineered cell types when used with the ʴǰ™ Transfection System
- Streamline your setup and post-mechanoporation handling with pre-assembled cartridges
- Use in combination with ʴǰ™ Delivery Media to support reproducible results from optimization through routine runs
ʴǰ™ Delivery Media
Description:
ʴǰ™ Delivery Media are specialized mechanoporation media designed for use with the ʴǰ™ Transfection System to facilitate cytosolic delivery into mammalian cells, including immune cells, HSPCs, and hPSCs. Its physiological formulation supports cargo stability and ensures high cell viability by providing a gentle environment during membrane deformation.
Advantages:
- Enable cytosolic delivery of nucleic acids, ribonucleoproteins, proteins, and other biomolecules when used with the ʴǰ™ Transfection System
- Enhance transfection efficiency and maintain cell viability during mechanoporation
- Standardize setup and optimize results by using within ʴǰ™ Delivery Cartridges
Collapse Details
Explore These Resources
Brochure
ʴǰ™ Transfection System Flyer
Brochure
Efficient Gene Editing of CD34+ HSPCs with ʴǰ™
Protocol
Gene Editing Human CD34+ Hematopoietic Stem and Progenitor Cells with the ʴǰ™ Transfection System
Legal Statement:
The purchase of a ʴǰ™ Transfection System, ʴǰ™ Delivery Cartridges, and associated reagents (“ʴǰ™ Products”) conveys to the purchaser a limited, non-exclusive, non-transferable license to use ʴǰ™ products, in accordance with ƽ’s Terms and Conditions of Sale (www.stemcell.com/terms-and-conditions-general) and all applicable laws and regulations, and solely for research-use-only applications (which applications exclude any commercial application or any therapeutic, prophylactic, diagnostic application, as well as any development and/or commercialization of products therewith or developed therefrom).
ʴǰ™, SQZ™, Cell Squeeze™, and Empower Cells to Change Lives™ are trademarks of ƽ Technologies Canada Inc., following its acquisition of SQZ Biotech. Nothing in this agreement grants the purchaser any rights to use these trademarks without prior written permission from ƽ. ƽ or its licensor(s) grant no additional license rights other than those explicitly granted herein, and except for such limited license rights, all other intellectual property and proprietary rights in and to ʴǰ™ products are and shall remain the exclusive property of ƽ and its licensors. The purchaser of ʴǰ™ products agrees to prevent unauthorized use, access, copying, or disclosure of any intellectual property comprised in ʴǰ™ products. The purchaser shall not, and shall not permit anyone to copy, create any derivative work, reverse engineer, disassemble, decompile, or make improvements to ʴǰ™ products. Users and purchasers of ʴǰ™ products hereby grant to ƽ exclusive ownership and all rights, title, and interest in and to any improvements made, by purchasers or users, to the ʴǰ™ products (including any improvements by purchasers or users to the intellectual property directly related to ʴǰ™ products), provided however that the user or purchaser who makes the improvement retains a limited, non-transferable license to use such improvement for its internal, non-commercial research use, in accordance with this agreement, only so long as such user or purchaser is in compliance with this agreement. All data, information, and results (to the extent they do not constitute improvements of ʴǰ™ products) entered, stored, compiled, generated, and/or analyzed by purchasers of ʴǰ™ products using the ʴǰ™ products in accordance with all of the foregoing shall be the property of such purchaser. ʴǰ™ Transfection System and ʴǰ™ Delivery Cartridges (“ʴǰ™ products”) are protected by patents in the U.S. and elsewhere. The list of products and patents contained herein are subject to change as a result of routine prosecution and dependent on the patent life cycle as determined by the laws of each jurisdiction in which a relevant patent is in effect.
Quality Statement:
UNLESS OTHERWISE STATED, PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES. FOR PRODUCT-SPECIFIC COMPLIANCE AND INTENDED USE INFORMATION, REFER TO THE PRODUCT INFORMATION SHEET. GENERAL INFORMATION ON QUALITY AT ƽ MAY BE FOUND AT WWW.ƽ.COM/COMPLIANCE.
The purchase of a ʴǰ™ Transfection System, ʴǰ™ Delivery Cartridges, and associated reagents (“ʴǰ™ Products”) conveys to the purchaser a limited, non-exclusive, non-transferable license to use ʴǰ™ products, in accordance with ƽ’s Terms and Conditions of Sale (www.stemcell.com/terms-and-conditions-general) and all applicable laws and regulations, and solely for research-use-only applications (which applications exclude any commercial application or any therapeutic, prophylactic, diagnostic application, as well as any development and/or commercialization of products therewith or developed therefrom).
ʴǰ™, SQZ™, Cell Squeeze™, and Empower Cells to Change Lives™ are trademarks of ƽ Technologies Canada Inc., following its acquisition of SQZ Biotech. Nothing in this agreement grants the purchaser any rights to use these trademarks without prior written permission from ƽ. ƽ or its licensor(s) grant no additional license rights other than those explicitly granted herein, and except for such limited license rights, all other intellectual property and proprietary rights in and to ʴǰ™ products are and shall remain the exclusive property of ƽ and its licensors. The purchaser of ʴǰ™ products agrees to prevent unauthorized use, access, copying, or disclosure of any intellectual property comprised in ʴǰ™ products. The purchaser shall not, and shall not permit anyone to copy, create any derivative work, reverse engineer, disassemble, decompile, or make improvements to ʴǰ™ products. Users and purchasers of ʴǰ™ products hereby grant to ƽ exclusive ownership and all rights, title, and interest in and to any improvements made, by purchasers or users, to the ʴǰ™ products (including any improvements by purchasers or users to the intellectual property directly related to ʴǰ™ products), provided however that the user or purchaser who makes the improvement retains a limited, non-transferable license to use such improvement for its internal, non-commercial research use, in accordance with this agreement, only so long as such user or purchaser is in compliance with this agreement. All data, information, and results (to the extent they do not constitute improvements of ʴǰ™ products) entered, stored, compiled, generated, and/or analyzed by purchasers of ʴǰ™ products using the ʴǰ™ products in accordance with all of the foregoing shall be the property of such purchaser. ʴǰ™ Transfection System and ʴǰ™ Delivery Cartridges (“ʴǰ™ products”) are protected by patents in the U.S. and elsewhere. The list of products and patents contained herein are subject to change as a result of routine prosecution and dependent on the patent life cycle as determined by the laws of each jurisdiction in which a relevant patent is in effect.
Quality Statement:
UNLESS OTHERWISE STATED, PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES. FOR PRODUCT-SPECIFIC COMPLIANCE AND INTENDED USE INFORMATION, REFER TO THE PRODUCT INFORMATION SHEET. GENERAL INFORMATION ON QUALITY AT ƽ MAY BE FOUND AT WWW.ƽ.COM/COMPLIANCE.
