STEMdiff™ Neural Progenitor Medium

Medium for maintenance and expansion of neural progenitor cells derived from human ES and iPS cells

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STEMdiff™ Neural Progenitor Medium

Medium for maintenance and expansion of neural progenitor cells derived from human ES and iPS cells

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Medium for maintenance and expansion of neural progenitor cells derived from human ES and iPS cells

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Product Advantages

Defined and serum-free

Supports expansion of NPCs generated using STEMdiff™ Neural Induction Medium

Optimized for efficient expansion of NPCs over multiple passages

Preserves NPC multipotency while minimizing spontaneous neuronal differentiation

Convenient, user-friendly format and protocol

What's Included

STEMdiff™ Neural Progenitor Basal Medium, 500 mL

STEMdiff™ Neural Progenitor Supplement A (50X), 10 mL

STEMdiff™ Neural Progenitor Supplement B (1000X), 500 µL

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Overview

STEMdiff™ Neural Progenitor Medium is a defined and serum-free medium for the expansion of neural progenitor cells (NPCs) derived from human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells using STEMdiff™ Neural Induction Medium (Catalog #05835). NPCs cultured in this medium can be expanded 3-5 fold per passage, and cultured for at least 10 passages, with minimal spontaneous neuronal differentiation.

Specialized Media

Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells

Human

Cell Culture, Expansion

STEMdiff

Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Stem Cell Biology

Serum-Free

More Information

More Information
Safety Statement	CA WARNING: This product can expose you to chemicals including Nickel Compounds which are known to the State of California to cause cancer and birth defects or other reproductive harm. For more information go to

Data Figures

Figure 1. Morphology and Marker Expression of Neural Progenitor Cells Cultured in STEMdiff™ Neural Progenitor Medium

(A) Typical NPC morphology is observed in cultures (shown at day 6 of passage 1). (B-D) NPCs maintained in STEMdiff™ Neural Progenitor Medium express the CNS-type NPC markers PAX6 (B, D, red), SOX1 (C, red) and NESTIN (C, green), but not the neural crest marker SOX10 (D, green, single channel shown in inset). B-D were taken at the same magnification.

Figure 2. Expansion of Neural Progenitor Cells in STEMdiff™ Neural Progenitor Medium

NPCs cultured in STEMdiff™ Neural Progenitor Medium can be expanded to generate a large number of cells. Three- to five-fold expansion can be achieved upon each passage. NPCs were derived using STEMdiff™ Neural Induction Medium and passaged once a week on average. n = 6.

Figure 3. Neural Progenitor Cells Cultured in STEMdiff™ Neural Progenitor Medium Show Minimal Spontaneous Neuronal Differentiation

Passages 1 (A) and 3 (B) of a representative NPC culture maintained in STEMdiff™ Neural Progenitor Medium. Cells were immunolabeled with SOX1 (red) to identify NPCs, and class III β-tubulin (green) to identify neurons. Spontaneous neuronal differentiation is low in NPC cultures maintained in STEMdiff™ Neural Progenitor Medium. A and B were taken at the same magnification.

Figure 4. Neural Progenitor Cells Maintained in STEMdiff™ Neural Progenitor Medium can Differentiate into Neurons and Astrocytes

When directed according to published protocols, NPCs can differentiate into neurons (A, class III β-tubulin shown in red) and astrocytes (B, GFAP shown in red). Nuclei are counterstained with DAPI (blue).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Name

Catalog #

05833

Lot #

All

Language

English

Document Type

Product Name

Catalog #

05833

Lot #

All

Language

English

Document Type

Product Name

Catalog #

05833

Lot #

All

Language

English

Document Type

Product Name

Catalog #

05833

Lot #

All

Language

English

Document Type

Product Name

Catalog #

05833

Lot #

All

Language

English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area

Workflow Stages

Workflow Stages for Human Pluripotent Stem Cell Research

Reprogramming

Maintenance

Differentiation

Workflow Stages for hPSC-Derived Neural Cell Research

Cell Sourcing

Neural Induction

Expansion

Differentiation and Maturation

Characterization

Resources and Publications

Endolysosomal processing of neuron-derived signaling lipids regulates autophagy and lipid droplet degradation in astrocytes J. N. Bhupana et al. Nature Communications 2025 May

Abstract

Dynamic regulation of metabolic activities in astrocytes is critical to meeting the demands of other brain cells. During neuronal stress, lipids are transferred from neurons to astrocytes, where they are stored in lipid droplets (LDs). However, it is not clear whether and how neuron-derived lipids trigger metabolic adaptation in astrocytes. Here, we uncover an endolysosomal function that mediates neuron-astrocyte transcellular lipid signaling. We identify Tweety homolog 1 (TTYH1) as an astrocyte-enriched endolysosomal protein that facilitates autophagic flux and LD degradation. Astrocyte-specific deletion of mouse Ttyh1 and loss of its Drosophila ortholog lead to brain accumulation of neutral lipids. Computational and experimental evidence suggests that TTYH1 mediates endolysosomal clearance of ceramide 1-phosphate (C1P), a sphingolipid that dampens autophagic flux and LD breakdown in mouse and human astrocytes. Furthermore, neuronal C1P secretion induced by inflammatory cytokine interleukin-1β causes TTYH1-dependent autophagic flux and LD adaptations in astrocytes. These findings reveal a neuron-initiated signaling paradigm that culminates in the regulation of catabolic activities in astrocytes. Subject terms: Organelles, Glial biology, Lipid signalling

CACNA1A loss-of-function affects neurogenesis in human iPSC-derived neural models I. Musante et al. Cellular and Molecular Life Sciences: CMLS 2025 Jun

Abstract

CACNA1A encodes the pore-forming α 1A subunit of the Ca V 2.1 calcium channel, whose altered function is associated with various neurological disorders, including forms of ataxia, epilepsy, and migraine. In this study, we generated isogenic iPSC-derived neural cultures carrying CACNA1A loss-of-function mutations differently affecting Ca V 2.1 splice isoforms. Morphological, molecular, and functional analyses revealed an essential role of CACNA1A in neurodevelopmental processes. We found that different CACNA1A loss-of-function mutations produce distinct neurodevelopmental deficits. The F1491S mutation, which is located in a constitutive domain of the channel and therefore causes a complete loss-of-function, impaired neural induction at very early stages, as demonstrated by changes in single-cell transcriptomic signatures of neural progenitors, and by defective polarization of neurons. By contrast, cells carrying the Y1854X mutation, which selectively impacts the synaptically-expressed Ca V 2.1[EFa] isoform, behaved normally in terms of neural induction but showed altered neuronal network composition and lack of synchronized activity. Our findings reveal previously unrecognized roles of CACNA1A in the mechanisms underlying neural induction and neural network dynamics and highlight the differential contribution of the divergent variants Ca V 2.1[EFa] and Ca V 2.1[EFb] in the development of human neuronal cells. The online version contains supplementary material available at 10.1007/s00018-025-05740-7.

Protein Kinase C promotes peroxisome biogenesis and peroxisome–endoplasmic reticulum interaction A. Borisyuk et al. The Journal of Cell Biology 2025 Jul

Abstract

Borisyuk et al. identify a signaling regulatory network of peroxisome proliferation, uncovering PKC as a positive regulator of peroxisome–ER interaction. During neuronal differentiation, activation of PKC contributes to an increase in peroxisome formation.

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT ��ƽ��, REFER TO WWW.��ƽ��.COM/COMPLIANCE.

Safety Statement:

CA WARNING: This product can expose you to chemicals including Nickel Compounds which are known to the State of California to cause cancer and birth defects or other reproductive harm. For more information go to

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STEMdiff™ Neural Progenitor Medium

Medium for maintenance and expansion of neural progenitor cells derived from human ES and iPS cells

STEMdiff™ Neural Progenitor Medium

Medium for maintenance and expansion of neural progenitor cells derived from human ES and iPS cells

Product Advantages

What's Included

Request Pricing

Overview

More Information

Data Figures

Protocols and Documentation

Applications

Resources and Publications

Educational Materials (14)

Publications (26)

Abstract

Abstract

Abstract

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