NeuroCult™ NS-A Neural Stem Cell Proliferation Kit

NeuroCult™ NS-A Proliferation Kit (Human)

Medium for expansion of human neural stem and progenitor cells

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NeuroCult™ NS-A Proliferation Kit (Human)

Medium for expansion of human neural stem and progenitor cells

Size

500 mL Request Pricing

Catalog #

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Medium for expansion of human neural stem and progenitor cells

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What's Included

NeuroCult™ NS-A Basal Medium (Human), 450 mL (Catalog #05750)

NeuroCult™ Proliferation Supplement (Human), 50 mL (Catalog #05753)

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To see all required products for your protocol, please consult the Protocols and Documentation.

Heparin Solution
Cell culture supplement
Human Recombinant bFGF
Basic fibroblast growth factor
Human Recombinant EGF
Epidermal growth factor
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

Overview

NeuroCult™ NS-A Proliferation Kit (Human) is a standardized, serum-free basal medium and supplement for the culture of human neural stem and progenitor cells from normal tissues or tumor samples, in the neurosphere or adherent monolayer system. When supplemented with appropriate cytokines, NeuroCult™ NS-A Proliferation Kit (Human) is optimized to maintain human neural stem cells in culture for extended periods of time without the loss of their self-renewal, proliferation, or differentiation potential.

NOTE: Addition of rh EGF (Catalog #78006), rh bFGF (Catalog #78003) and heparin (Catalog #07980) is required.

Specialized Media

Brain Tumor Stem Cells, Neural Stem and Progenitor Cells

Human

Cell Culture, Colony Assay, Expansion, Functional Assay, Spheroid Culture, Toxicity Assay

NeuroCult

Cancer, Drug Discovery and Toxicity Testing, Neuroscience, Stem Cell Biology

Serum-Free

More Information

More Information
Safety Statement	CA WARNING: This product can expose you to Progesterone which is known to the State of California to cause cancer. For more information go to

Data Figures

Total cell expansion for fetal human telencephalic and cortical cell neurospheres cultured with Complete NeuroCult™ Proliferation Medium (Human) containing rh EGF, rh bFGF and heparin

Figure 1. Total Cell Expansion for Fetal Human Telencephalic and Cortical Cells Cultured as Neurospheres with Complete NeuroCult™ Proliferation Medium (Human) Containing rh EGF, rh bFGF and Heparin

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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05751

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English

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05751

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English

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05751

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English

Document Type

Product Name

Catalog #

05751

Lot #

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English

Document Type

Product Name

Catalog #

05751

Lot #

All

Language

English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area

Workflow Stages

Workflow Stages for Brain Tumor Stem Cell Research

Tissue Dissociation

Expansion

Differentiation

Characterization

Workflow Stages for Neural Stem and Progenitor Cell Research

Tissue Dissociation

Expansion

Differentiation

Characterization

Resources and Publications

Invasion of glioma cells through confined space requires membrane tension regulation and mechano-electrical coupling via Plexin-B2 Nature Communications 2025 Jan

Abstract

Glioblastoma (GBM) is a malignant brain tumor with diffuse infiltration. Here, we demonstrate how GBM cells usurp guidance receptor Plexin-B2 for confined migration through restricted space. Using live-cell imaging to track GBM cells negotiating microchannels, we reveal endocytic vesicle accumulation at cell front and filamentous actin assembly at cell rear in a polarized manner. These processes are interconnected and require Plexin-B2 signaling. We further show that Plexin-B2 governs membrane tension and other membrane features such as endocytosis, phospholipid composition, and inner leaflet surface charge, thus providing biophysical mechanisms by which Plexin-B2 promotes GBM invasion. Together, our studies unveil how GBM cells regulate membrane tension and mechano-electrical coupling to adapt to physical constraints and achieve polarized confined migration. The biomechanical mechanisms enabling the invasive growth of brain tumors remain opaque. Here, Junqueira Alves et al. reveal that the guidance receptor Plexin-B2 controls membrane tension, facilitating confined migration of brain tumor cells.

Single-cell RNA-seq reveals that glioblastoma recapitulates a normal neurodevelopmental hierarchy. C. P. Couturier et al. Nature communications 2020 jul

Abstract

Cancer stem cells are critical for cancer initiation, development, and treatment resistance. Our understanding of these processes, and how they relate to glioblastoma heterogeneity, is limited. To overcome these limitations, we performed single-cell RNA sequencing on 53586 adult glioblastoma cells and 22637 normal human fetal brain cells, and compared the lineage hierarchy of the developing human brain to the transcriptome of cancer cells. We find a conserved neural tri-lineage cancer hierarchy centered around glial progenitor-like cells. We also find that this progenitor population contains the majority of the cancer's cycling cells, and, using RNA velocity, is often the originator of the other cell types. Finally, we show that this hierarchal map can be used to identify therapeutic targets specific to progenitor cancer stem cells. Our analyses show that normal brain development reconciles glioblastoma development, suggests a possible origin for glioblastoma hierarchy, and helps to identify cancer stem cell-specific targets.

BMP signaling mediates glioma stem cell quiescence and confers treatment resistance in glioblastoma R. Sachdeva et al. Scientific Reports 2019 dec

Abstract

Despite advances in therapy, glioblastoma remains an incurable disease with a dismal prognosis. Recent studies have implicated cancer stem cells within glioblastoma (glioma stem cells, GSCs) as mediators of therapeutic resistance and tumor progression. In this study, we investigated the role of the transforming growth factor-$\beta$ (TGF-$\beta$) superfamily, which has been found to play an integral role in the maintenance of stem cell homeostasis within multiple stem cell systems, as a mediator of stem-like cells in glioblastoma. We find that BMP and TGF-$\beta$ signaling define divergent molecular and functional identities in glioblastoma, and mark relatively quiescent and proliferative GSCs, respectively. Treatment of GSCs with BMP inhibits cell proliferation, but does not abrogate their stem-ness, as measured by self-renewal and tumorigencity. Further, BMP pathway activation confers relative resistance to radiation and temozolomide chemotherapy. Our findings define a quiescent cancer stem cell population in glioblastoma that may be a cellular reservoir for tumor recurrence following cytotoxic therapy.

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Related Products

Legal Statement:

Sold under license from StemCells California, Inc. US Patent Nos. 5,750,376; 5,851,832; 5,980,885; 5,968,829; 5,981,165; 6,071,889; 6,093,531; 6,103,530; 6,165,783; 6,238,922.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT ��ƽ��, REFER TO WWW.��ƽ��.COM/COMPLIANCE.

Safety Statement:

CA WARNING: This product can expose you to Progesterone which is known to the State of California to cause cancer. For more information go to

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NeuroCult™ NS-A Proliferation Kit (Human)

Medium for expansion of human neural stem and progenitor cells

NeuroCult™ NS-A Proliferation Kit (Human)

Medium for expansion of human neural stem and progenitor cells

What's Included

Request Pricing

Overview

More Information

Data Figures

Protocols and Documentation

Applications

Resources and Publications

Educational Materials (14)

Publications (113)

Abstract

Abstract

Abstract

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