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Human iPSC-Derived Endothelial Cells

Frozen endothelial cells differentiated from human induced pluripotent stem cell line, SCTi003-A

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Human iPSC-Derived Endothelial Cells

Frozen endothelial cells differentiated from human induced pluripotent stem cell line, SCTi003-A

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Frozen endothelial cells differentiated from human induced pluripotent stem cell line, SCTi003-A
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Product Advantages


  • Obtain high-quality human endothelial cells immediately post-thaw

  • Easily expand cells with STEMdiffâ„¢ Endothelial Expansion Culture Kit while retaining proliferation ability and quality

  • Ensure reproducibility by starting with highly pure ECs derived from the highly characterized hiPSC control line, SCTi003-A

Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Animal Component-Free Cell Dissociation Kit
    Animal Component-Free Cell Dissociation Kit

    Dissociation kit for human stem and progenitor cells

  2. Heparin Solution
    Heparin Solution

    Cell culture supplement

  3. STEMdiff™ Endothelial Expansion Culture Kit (Catalog #100-1218)
    STEMdiffâ„¢ Endothelial Expansion Culture Kit

    Expand human pluripotent stem cell-derived endothelial cells

  4. D-PBS (Without Ca++ and Mg++)
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

Overview

Start your vascular research confidently with high-quality, ready-to-use Human iPSC-Derived Endothelial Cells (ECs). These cryopreserved ECs are derived from the Healthy Control Human iPSC Line, Female, SCTi003-A using STEMdiffâ„¢ Mesoderm Induction Medium and STEMdiffâ„¢ Endothelial Differentiation Kit . Starting with differentiated cells allows you to obtain large numbers of cells for your research quickly.
Scale up your experiments by expanding thawed ECs using STEMdiffâ„¢ Endothelial Expansion Culture Kit for at least three passages while retaining proliferation ability. Expanded cells exhibit high expression of the endothelial-specific markers CD31, CD144, and CD309 (VEGFR2) while remaining free of hematopoietic (CD45) and epithelial (CD326) markers.
This research-use-only (RUO) product has been approved for both academic and commercial use. Blood samples are ethically sourced using Institutional Review Board (IRB) or other regulatory authority-approved consent forms and protocols. For donor details and cell quality characterization of the source cell banks, refer to the data figures on this page.
For additional details, refer to Frequently Asked Questions about iPSC Lines.
Species
Human
Cell and Tissue Source
Pluripotent Stem Cells
Application
Functional Assay

Data Figures

Figure 1. Showing Microscopy Image and Data Figures

Figure 1. Human iPSC-Derived Endothelial Cells Exhibit High-Quality Morphology and Robust Expansion for Multiple Passages

(A) Representative microscopy image showing cryopreserved human iPSC-derived endothelial cells thawed and maintained in STEMdiffâ„¢ Endothelial Expansion Medium for 4 days and ready for passaging at ~90 - 100% confluency.

(B) Cells show robust expansion post-thaw. Data points show the fold increase over 4 passages post-thaw from one technical replicate.

(C) Human iPSC-derived endothelial cells were thawed and maintained in STEMdiffâ„¢ Endothelial Expansion Medium. Cells were passaged every 3 - 5 days post-thaw, and cell expansion was analyzed as cumulative cell number over 4 passages from one technical replicate.

Figure 2. Human iPSC-Derived Endothelial Cells Express Characteristic Markers

Figure 2. Human iPSC-Derived Endothelial Cells Express Characteristic Markers

Human iPSC-derived endothelial cells were thawed, cultured in STEMdiffâ„¢ Endothelial Expansion Medium for four passages, and characterized using flow cytometry for endothelial markers. The cells show high expression of (A) CD31/CD144, (B) CD309, and (C) CD105. (D) A representative flow cytometry plot shows that the cells were negative for the epithelial marker CD326 (EpCAM).

Figure 3. Human iPSC-Derived Endothelial Cells Are Functional and Mature

Figure 3. Human iPSC-Derived Endothelial Cells Are Functional and Mature

Human iPSC-derived endothelial cells were thawed and cultured in STEMdiffâ„¢ Endothelial Expansion Medium for two passages before assessing functionality and maturity.

(A) Immunohistochemistry confirmed the expression of endothelial markers CD31 (green), VWF (magenta), and DAPI (gray), and (B) the ability to take up acetylated LDL (green) and Hoechst 33342 (Blue).

(C) Cells were able to form tubular networks in vitro in a tube formation assay when plated on Corning® Matrigel® for 24 hours.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
200-0907
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Legal Statement:

These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) shall not be used or administered in (1) human subjects for human clinical use; (2) animals for veterinary use for therapeutic, diagnostic, or prophylactic purposes or (3) any subject in relation to clinical applications, cell therapy, transplantation, and/or regenerative medicines, without limiting the generality of the foregoing. These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for monetization or commercialization purposes, including without limitation, used to, or with the goal to, perform services or supply products or rights, including in the manufacture of cellular therapies or other therapeutics, for monetary gain or the generation of royalties, revenues, sales or other valuable consideration. For clarity, these iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for screening compounds, antibodies, proteins or peptides, except for the purposes of target discovery, target validation, or assay development, provided such activities and the results of such activities are not further used for monetization or commercialization purposes. It may be possible to obtain a further license for the prohibited uses referred to in this Limited Use License. Please contact iPSCrequests@stemcell.com for more details.



Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.

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