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EasySep? Non-Human Primate B Cell Isolation Kit

Immunomagnetic negative isolation of untouched non-human primate B cells from non-human primate PBMCs

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EasySep? Non-Human Primate B Cell Isolation Kit

Immunomagnetic negative isolation of untouched non-human primate B cells from non-human primate PBMCs

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Immunomagnetic negative isolation of untouched non-human primate B cells from non-human primate PBMCs
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Product Advantages


  • Fast, easy to use, and column-free

  • Up to 95% purity

  • Untouched, viable cells

What's Included

  • EasySep? Non-Human Primate B Cell Isolation Kit (Catalog #100-0345)
    • EasySep? Non-Human Primate B Cell Isolation Cocktail, 1 mL
    • EasySep? Dextran RapidSpheres?, 2 x 1 mL
    • EasySep? Isolation Cocktail Enhancer, 1 mL
  • RoboSep? Non-Human Primate B Cell Isolation Kit (Catalog #100-0347)
    • EasySep? Non-Human Primate B Cell Isolation Cocktail, 1 mL
    • EasySep? Dextran RapidSpheres?, 2 x 1 mL
    • EasySep? Isolation Cocktail Enhancer, 1 mL
    • RoboSep? Buffer (Catalog #20104)
    • RoboSep? Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer for use with EasySep? cell separation kits

  3. Anti-Human CD20 Antibody, Clone 2H7
    Anti-Human CD20 Antibody, Clone 2H7

    Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD20

Overview

Easily and efficiently isolate highly purified non-human primate B cells from fresh or previously frozen non-human primate peripheral blood mononuclear cells (PBMCs) by immunomagnetic negative selection, with the EasySep? Non-Human Primate B Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: T cells, NK cells, monocytes, granulocytes, mast cells, dendritic cells, hematopoietic progenitors, and platelets. The magnetically labeled cells are then separated from the untouched desired B cells by using an EasySep? magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 20 minutes, the desired B cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This kit has been verified for use with rhesus and cynomolgus macaques.

Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep?. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
? EasySep? Magnet (Catalog #18000)
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? RoboSep?-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Non-Human Primate
Sample Source
PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology, Infectious Diseases

Data Figures

The content of rhesus macaque CD20+ B cells before and after isolation correspond to 10.4% and 94.8%, respectively.

Figure 1. EasySep? Non-Human Primate B Cell Isolation Kit

Starting with rhesus PBMCs, the B cell content (CD20+) of the isolated fraction is typically 91.4 ± 5.2% (mean ± SD using the purple EasySep? Magnet). In the above example, the purities of the start and final isolated fractions are 10.4% and 94.8%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
100-0345
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-0345
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-0345
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-0345
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-0347
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (5)

Brochure
Brochure
Brochure
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

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Quality Statement:

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