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EasySep? Non-Human Primate T Cell Isolation Kit

Immunomagnetic nagative selection of untouched T cells from non-human primates including rhesus macaque

EasySep? Non-Human Primate T Cell Isolation Kit

Immunomagnetic nagative selection of untouched T cells from non-human primates including rhesus macaque

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Immunomagnetic nagative selection of untouched T cells from non-human primates including rhesus macaque
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Product Advantages


  • Fast, easy to use, and column-free

  • Up to 90% purity with high recovery

  • Untouched, viable cells

What's Included

  • EasySep? Non-Human Primate T Cell Isolation Kit (Catalog #19581)
    • EasySep? Non-Human Primate T Cell Isolation Cocktail, 1 mL
    • EasySep? D Magnetic Particles, 2 x 1 mL
  • RoboSep? Non-Human Primate T Cell Isolation Kit (Catalog #19581RF)
    • EasySep? Non-Human Primate T Cell Isolation Cocktail, 1 mL
    • EasySep? D Magnetic Particles, 2 x 1 mL
    • RoboSep? Buffer (Catalog #20104)
    • RoboSep? Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

Easily and efficiently isolate highly purified pan T cells from fresh or previously frozen non-human primate peripheral blood mononuclear cell (PBMC) samples by immunomagnetic negative selection, with the EasySep? Non-Human Primate T Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: B cells, NK cells, monocytes, granulocytes, mast cells, dendritic cells, hematopoietic progenitors, and platelets. The magnetically labeled cells are then separated from the untouched desired T cells by using an EasySep? magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 20 minutes, the desired T cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This kit has been verified for use with rhesus and cynomolgus macaques.

Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep?. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
? EasySep? Magnet (Catalog #18000)
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? RoboSep?-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells
Species
Non-Human Primate
Sample Source
PBMC, Peripheral Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
HIV, Immunology, Infectious Diseases

Data Figures

The content of rhesus macaque CD3+ T cells before and after isolation correspond to 55.7% and 93.9%, respectively.

Figure 1. EasySep? Non-Human Primate T Cell Isolation Kit

In this example, starting with rhesus macaque PBMCs, the T cell content (within the CD45+ gate) of start and final isolated fractions are 55.7% and 93.9%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
19581
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19581RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19581
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19581
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19581RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19581RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19581RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.