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Easily and efficiently isolate highly purified pan T cells from fresh or previously frozen non-human primate peripheral blood mononuclear cell (PBMC) samples by immunomagnetic negative selection, with the EasySep? Non-Human Primate T Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep? negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: B cells, NK cells, monocytes, granulocytes, mast cells, dendritic cells, hematopoietic progenitors, and platelets. The magnetically labeled cells are then separated from the untouched desired T cells by using an EasySep? magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 20 minutes, the desired T cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
This kit has been verified for use with rhesus and cynomolgus macaques.
Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep?. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Figure 1. EasySep? Non-Human Primate T Cell Isolation Kit
In this example, starting with rhesus macaque PBMCs, the T cell content (within the CD45+ gate) of start and final isolated fractions are 55.7% and 93.9%, respectively.
This product is designed for use in the following research area(s) as part
of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we
offer to support each research area.
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD8a
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EasySep? Non-Human Primate T Cell Isolation Kit
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