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EasySep? Human Pan-CD25 Positive Selection and Depletion Kit

Immunomagnetic positive selection or depletion of human CD25+ cells from fresh PBMCs or lysed leukapheresis samples

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EasySep? Human Pan-CD25 Positive Selection and Depletion Kit

Immunomagnetic positive selection or depletion of human CD25+ cells from fresh PBMCs or lysed leukapheresis samples

Catalog #
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Immunomagnetic positive selection or depletion of human CD25+ cells from fresh PBMCs or lysed leukapheresis samples
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Product Advantages


  • Fast, easy-to-use and column-free

  • Up to 1.3 log depletion of CD25+ cells

  • Up to 98% purity

What's Included

EasySep? Human Pan-CD25 Positive Selection and Depletion Kit (Catalog #17861)
    • EasySep?  Human Pan-CD25 Positive Selection and Depletion Cocktail, 1 mL
    • EasySep? Dextran RapidSpheres? 50100, 2 x 1 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer for use with EasySep? cell separation kits

Overview

Isolate or deplete human CD25+ cells from fresh human peripheral blood mononuclear cells (PBMCs) or lysed leukapheresis samples with ease, using immunomagnetic positive selection, with the EasySep? Human Pan-CD25 Positive Selection and Depletion Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? positive selection procedure, cells of interest are labeled with antibody complexes recognizing CD25 and magnetic particles. Labeled cells are separated using an EasySep? magnet and by simply pouring off the unlabeled cells. The CD25+ cells remain in the tube. Following magnetic cell isolation, the desired cells from either fraction are ready for downstream applications, such as flow cytometry, in vitro assays, or cell culture. The CD25 antigen is expressed on regulatory T cells and activated T and B cells.

Learn more about how immunomagnetic EasySep? technology works. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
? EasySep? Magnet (Catalog #18000)
? “The Big Easy” EasySep? Magnet (Catalog #18001)
Subtype
Cell Isolation Kits
Cell Type
T Cells, CD4+, T Cells, Regulatory
Species
Human
Sample Source
PBMC
Selection Method
Depletion
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Data Figures

CD25 cell depletion results using EasySep?

Starting with fresh PBMCs, the CD25+ cell content of the depleted fraction typically ranges from 2 - 5%. In the above example, the purities of the start and final depleted fractions are 14.5% and 2.2%, respectively, with a 1.3 log depletion of CD25+ cells. Using the optional positive selection protocol, the CD25+ cell content of the isolated fraction typically ranges from 81 - 98% (data not shown).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
17861
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17861
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17861
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (10)

Brochure
Brochure
Brochure
Regulatory T Cells
Wallchart
Regulatory T Cells
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll? or Lymphoprep?)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll? or Lymphoprep...
Isolate Cells with a Simple Pour-Off: EasySep? Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep? Cell Separation Technology

Publications (2)

CRISPR/Cas9-Correctable mutation-related molecular and physiological phenotypes in iPSC-derived Alzheimer's PSEN2 N141I neurons. M. Ortiz-Virumbrales et al. Acta neuropathologica communications 2017 dec

Abstract

Basal forebrain cholinergic neurons (BFCNs) are believed to be one of the first cell types to be affected in all forms of AD, and their dysfunction is clinically correlated with impaired short-term memory formation and retrieval. We present an optimized in vitro protocol to generate human BFCNs from iPSCs, using cell lines from presenilin 2 (PSEN2) mutation carriers and controls. As expected, cell lines harboring the PSEN2 N141I mutation displayed an increase in the A$\beta$42/40 in iPSC-derived BFCNs. Neurons derived from PSEN2 N141I lines generated fewer maximum number of spikes in response to a square depolarizing current injection. The height of the first action potential at rheobase current injection was also significantly decreased in PSEN2 N141I BFCNs. CRISPR/Cas9 correction of the PSEN2 point mutation abolished the electrophysiological deficit, restoring both the maximal number of spikes and spike height to the levels recorded in controls. Increased A$\beta$42/40 was also normalized following CRISPR/Cas-mediated correction of the PSEN2 N141I mutation. The genome editing data confirms the robust consistency of mutation-related changes in A$\beta$42/40 ratio while also showing a PSEN2-mutation-related alteration in electrophysiology.
Combinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation. Albert BJ et al. Scientific reports 2017 AUG

Abstract

Current antiretroviral therapy (ART) for HIV/AIDS slows disease progression by reducing viral loads and increasing CD4 counts. Yet ART is not curative due to the persistence of CD4+ T-cell proviral reservoirs that chronically resupply active virus. Elimination of these reservoirs through the administration of synergistic combinations of latency reversing agents (LRAs), such as histone deacetylase (HDAC) inhibitors and protein kinase C (PKC) modulators, provides a promising strategy to reduce if not eradicate the viral reservoir. Here, we demonstrate that largazole and its analogues are isoform-targeted histone deacetylase inhibitors and potent LRAs. Significantly, these isoform-targeted HDAC inhibitors synergize with PKC modulators, namely bryostatin-1 analogues (bryologs). Implementation of this unprecedented LRA combination induces HIV-1 reactivation to unparalleled levels and avoids global T-cell activation within resting CD4+ T-cells.
View All Publications

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