Liver Epithelial Cell Research
Liver stem cells are dormant and generally not detectable in the healthy adult liver. However, injury triggers the activation of an Lgr5+ epithelial progenitor cell population. Single Lgr5+ cells from adult mouse liver can be isolated and expanded into a 3D culture system to generate hepatic organoids that possess clonogenic potential and can be maintained long-term. Use to establish and maintain hepatic organoids from mouse ductal fragments. For human cultures, Epcam+ bile duct-derived bipotent progenitors can be isolated for expansion into human hepatic organoids. Use to generate mature hepatic organoids from fresh or frozen human liver tissue.
Products
Cell Culture Media and Supplements 4 (1)
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Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer
Cell Dyes and Detection Assay Kits 4 (2)
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DNA-labeling dye
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Reagent for counting viable mammalian cells
Buffers and Solutions 4 (1)
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Dulbecco’s phosphate-buffered saline without calcium and magnesium
Tissue and Cell Culture Dissociation Reagents 4 (3)
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Animal component-free collagenase for the digestion of native collagen fibrils
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For digestion of native collagen fibrils
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cGMP, enzyme-free cell dissociation reagent
Cultureware and General Supplies 4 (1)
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Reversible strainer for filtration of single cells and isolation of cellular aggregates, including embryoid bodies and spheroids
