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Easily and efficiently isolate highly purified human memory CD4+ T cells (CD4+CD45RA-CD45RO+) from fresh or previously frozen human peripheral blood mononuclear cell (PBMC) samples by immunomagnetic negative selection, with the EasySep? Human Memory CD4+ T Cell Enrichment Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. Unwanted cells expressing the following markers are targeted for removal: CD8, CD14, CD16, CD19, CD20, CD36, CD45RA, CD56, CD123, TCR¦Ã/¦Ä, and glyA. The magnetically labeled cells are then separated from the untouched desired memory CD4+ T cells by using an EasySep? magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation , the desired memory CD4+ T cells are ready for downstream applications such as e.g. flow cytometry, culture, or DNA/RNA extraction.

Learn more about how immunomagnetic works or how to fully automate immunomagnetic cell isolation with RoboSep?. Alternatively, choose ready-to-use, ethically sourced, primary Human Peripheral Blood CD4+CD45RO+ T Cells, Frozen isolated with EasySep? Human Memory CD4+ T Cell Enrichment Kit. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.