海角破解版

  • Compare Products
Menu
Account
Make more informed purchasing decisions with our new product availability and delivery estimate feature, now available on all product pages, in your cart, and during checkout.

EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit

Immunomagnetic isolation of untouched human CD4+CD127lowCD49d- T cells from PBMCs

Loading...
 
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit

Immunomagnetic isolation of untouched human CD4+CD127lowCD49d- T cells from PBMCs

Catalog #
(Select a product)
Immunomagnetic isolation of untouched human CD4+CD127lowCD49d- T cells from PBMCs
Request Pricing

Product Advantages


  • Fast, easy-to-use and column-free

  • Up to 87.4% purity

  • Isolated cells are untouched

What's Included

  • EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit (Catalog #19232)
    • EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Cocktail, 1 mL
    • EasySep? Dextran Selection Cocktail, 1 mL
    • EasySep? Magnetic Particles, 3 x 1 mL
  • RoboSep? CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit (Catalog #19232RF)
    • EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Cocktail, 1 mL
    • EasySep? Dextran Selection Cocktail, 1 mL
    • EasySep? Magnetic Particles, 3 x 1 mL
    • RoboSep? Buffer (Catalog #20104)
    • RoboSep? Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer for use with EasySep? cell separation kits

Overview

Easily and efficiently isolate highly purified CD4+CD127lowCD49d- T cells from fresh human peripheral blood mononuclear cells (PBMCs) by immunomagnetic negative selection, with the EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The magnetically labeled cells are then separated from the untouched desired CD4+CD127lowCD49d- T cells by using an EasySep? magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired CD4+CD127lowCD49d- T cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Learn more about how immunomagnetic works or how to fully automate immunomagnetic cell isolation with RoboSep?. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
? EasySep? Magnet (Catalog #18000)
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? RoboSep?-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD4+, T Cells, Regulatory
Species
Human
Sample Source
PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Data Figures

Typical EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Profile

Figure 1. Typical EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Profile

Starting with fresh PBMCs, the CD3+CD4+CD127lowCD49d-CD25+FOXP3+ cell content of the enriched fraction typically ranges from 57.4 - 87.4%. Donor to donor variability is unavoidable and may affect the purity of the enriched fraction. In the example above, the purities of the start and enriched fractions are 1.84% and 84.1%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
19232
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (10)

Brochure
Brochure
Human Immune Cytokines
Wallchart
Human Immune Cytokines