海角破解版

  • Compare Products
Menu
Account
Maximize your efficiency in the lab with free downloadable templates, spreadsheets, and checklists!   Access Now >

EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit

Immunomagnetic isolation of untouched human CD4+CD127lowCD49d- T cells from PBMCs

Loading...
 
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit

Immunomagnetic isolation of untouched human CD4+CD127lowCD49d- T cells from PBMCs

Catalog #
(Select a product)
Immunomagnetic isolation of untouched human CD4+CD127lowCD49d- T cells from PBMCs
Request Pricing Request Pricing

Product Advantages


  • Fast, easy-to-use and column-free

  • Up to 87.4% purity

  • Isolated cells are untouched

What's Included

  • EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit (Catalog #19232)
    • EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Cocktail, 1 mL
    • EasySep? Dextran Selection Cocktail, 1 mL
    • EasySep? Magnetic Particles, 3 x 1 mL
  • RoboSep? CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit (Catalog #19232RF)
    • EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Cocktail, 1 mL
    • EasySep? Dextran Selection Cocktail, 1 mL
    • EasySep? Magnetic Particles, 3 x 1 mL
    • RoboSep? Buffer (Catalog #20104)
    • RoboSep? Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer for use with EasySep? cell separation kits

Overview

Easily and efficiently isolate highly purified CD4+CD127lowCD49d- T cells from fresh human peripheral blood mononuclear cells (PBMCs) by immunomagnetic negative selection, with the EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The magnetically labeled cells are then separated from the untouched desired CD4+CD127lowCD49d- T cells by using an EasySep? magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired CD4+CD127lowCD49d- T cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep?. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
? EasySep? Magnet (Catalog #18000)
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? RoboSep?-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD4+, T Cells, Regulatory
Species
Human
Sample Source
PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

Typical EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Profile

Figure 1. Typical EasySep? Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Profile

Starting with fresh PBMCs, the CD3+CD4+CD127lowCD49d-CD25+FOXP3+ cell content of the enriched fraction typically ranges from 57.4 - 87.4%. Donor to donor variability is unavoidable and may affect the purity of the enriched fraction. In the example above, the purities of the start and enriched fractions are 1.84% and 84.1%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
19232
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19232RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (10)

Brochure
Brochure
Regulatory T Cells
Wallchart
Regulatory T Cells
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll? or Lymphoprep?)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll? or Lymphoprep...
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
Scientific Poster
Scientific Poster
Scientific Poster

Frequently Asked Questions

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Publications (1)

A Transendocytosis Model of CTLA-4 Function Predicts Its Suppressive Behavior on Regulatory T Cells Hou TZ et al. The Journal of Immunology 2015

Abstract

Manipulation of the CD28/CTLA-4 pathway is at the heart of a number of immunomodulatory approaches used in both autoimmunity and cancer. Although it is clear that CTLA-4 is a critical regulator of T cell responses, the immunological contexts in which CTLA-4 controls immune responses are not well defined. In this study, we show that whereas CD80/CD86-dependent activation of resting human T cells caused extensive T cell proliferation and robust CTLA-4 expression, in this context CTLA-4 blocking Abs had no impact on the response. In contrast, in settings where CTLA-4(+) cells were present as regulators?
View All Publications

Related Products

Related Products

Labeling Antibodies

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT 海角破解版, REFER TO WWW.海角破解版.COM/COMPLIANCE.

Copyright ? 2025 海角破解版. All rights reserved.