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EasySep™ HLA Whole Blood T Cell Enrichment Kit

Immunomagnetic negative selection of untouched human T cells from ٲ™-treated whole blood or washed leukapheresis samples

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ HLA Whole Blood T Cell Enrichment Kit

Immunomagnetic negative selection of untouched human T cells from ٲ™-treated whole blood or washed leukapheresis samples

Catalog #
(Select a product)
Immunomagnetic negative selection of untouched human T cells from ٲ™-treated whole blood or washed leukapheresis samples
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Product Advantages


  • Fast, easy-to-use and column-free

  • Up to 98% purity

  • Untouched, viable cells

  • Compatible with downstream HLA assays

What's Included

  • EasySep™ HLA Whole Blood T Cell Enrichment Kit (Catalog #19951HLA)
    • EasySep™ HLA Whole Blood T Cell Enrichment Cocktail, 1 mL
    • EasySep™ Magnetic Particles, 3 x 1 mL
    • ٲ™, 20 mL (Catalog #07806) x 2
  • Dzdz™ HLA Whole Blood T Cell Enrichment Kit with Filter Tips (Catalog #19951HLARF)
    • EasySep™ HLA Whole Blood T Cell Enrichment Cocktail, 1 mL
    • EasySep™ Magnetic Particles, 3 x 1 mL
    • ٲ™, 20 mL (Catalog #07806) x 2
    • Dzdz™ Buffer (Catalog #20104)
    • Dzdz™ Filter Tips (Catalog #20125) x 2
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer for use with EasySep™ cell separation kits

Overview

Easily and efficiently isolate highly purified human T cells from ٲ™-treated whole blood or washed leukapheresis samples by immunomagnetic negative selection, with the EasySep™ HLA Whole Blood T Cell Enrichment Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. Unwanted cells expressing the following markers are targeted for removal: CD14, CD16, CD19, CD20, CD33, CD36, CD42b, CD56, CD66b, and CD123. The magnetically labeled cells are then separated from the untouched desired T cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired T cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Please Note: In light of the new EU Regulation 2017/746 (IVDR) of the European Parliament and of the Council on in vitro diagnostic medical devices, which became effective in 2022, ƽ deregistered and removed the CE IVD claims associated with these products, and they are now labeled “For Research Use Only”. Please note there have been no changes to the form, function, and quality assurances associated with these products. For more information, please contact the Quality Assurance and Regulatory Department at qaschangenote@stemcell.com.

The EasySep™ Direct HLA B Cell Isolation Kit (Catalog #89684) is available for sale in Canada, the EU, UK, and Australia. In all other regions, the EasySep™ Direct HLA Crossmatch B Cell Isolation Kit (Catalog #19684) is available as an equivalent product.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with Dzdz™ to save time and increase laboratory throughput. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Dzdz™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells
Species
Human
Sample Source
Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Chimerism, HLA, Immunology

Data Figures

FACS Histogram Results with EasySep™ HLA Whole Blood T Cell Enrichment Kit

Figure 1. FACS Histogram Results with EasySep™ HLA Whole Blood T Cell Enrichment Kit

Starting with HetaSep™-treated whole blood, the CD3+ cell content of the enriched fraction typically ranges from 93.1 - 98.0% (gated on CD45+ cells). Starting with 0.5 mL of HetaSep™-treated whole blood (equivalent to 5 mL of whole blood), 1.1 - 3.1 x 10e6 CD3+ cells are typically recovered.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
19951HLARF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19951HLA
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19951HLARF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19951HLARF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19951HLARF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19951HLARF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19951HLA
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19951HLA
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19951HLA
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (8)

Brochure
Brochure
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Automate Cell Isolation with the Dzdz™-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the Dzdz™-S Cell Separation Instrument
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Scientific Poster
Scientific Poster

Frequently Asked Questions

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT ƽ, REFER TO WWW.ƽ.COM/COMPLIANCE.

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