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The video demonstrates how to cryopreserve cells of interest, such as human peripheral blood mononuclear cells or PBMCs, using CryoStor® CS10 freezing medium, available from �����ƽ�� Technologies. CryoStor® CS10 is cGMP-manufactured and is a ready-to-use, serum-, animal component-, and protein-free medium to ensure both consistent results and safety with sensitive cell types. It's designed to maintain high viability and maximize cell recovery after long-term cryopreservation or short-term hypothermic storage. CryoStor® can be used for freezing down various cell types, including T cells and NK cells. CryoStor® can also be used to cryopreserve cells derived from pluripotent stem cells and various other sources, including tissue samples, human embryonic stem cells, and induced pluripotent stem cells. In this video, we will take you through �����ƽ�� Technologies' protocol on how to use CryoStor® CS10 to freeze human peripheral blood mononuclear cells or PBMCs. To freeze down PBMCs, start by sourcing your fresh peripheral blood-derived normal or diseased primary cells. Next, collect the cells and ensure your PBMCs or other isolated subsets are in a single cell suspension. Transfer your cells to a tube in preparation for a centrifuge. Centrifuge your cells to obtain a cell pellet. Protocols will vary depending on cell type. In a biosafety cabinet, remove the supernatant. Resuspend in chilled CryoStor® CS10 to the optimal cell concentration, depending on the cell type, and mix thoroughly. Mixing is normally done by gentle resuspension. Transfer the cell suspension to a cryovial and label the vial with any necessary information. We recommend incubating the cells at 2–8°C for 10 minutes prior to cryopreservation to allow for cell adaptation. Cryopreserve cells in a -80°C freezer using an alcohol-free freezing container that achieves rate-controlled freezing, such as the CoolCell® LX Cell Freezing Container. Alternatively, use a standard rate-controlled cooling protocol, approximately -1°C per minute in a rate-controlled freezer to store at -135°C. For long-term storage, transfer the vials of frozen cells from the -80°C freezer or freezing container to vapor phase liquid nitrogen. Minimize exposure to room temperature by placing vials on dry ice during transfer to liquid nitrogen. When you're ready to use your frozen cells, thawing them correctly is critical for successful post-thaw experiments or downstream applications. Ensure sample sterility and gain consistent results with the ThawSTAR® CFT2 Automated Thawing System. CryoStor® Freezing Medium is made with high-quality USP-grade components for reliable results. The majority of frozen human primary cells sold �����ƽ�� are cryopreserved in CS10 to provide a safe, protective environment for a variety of sensitive cell and sample types. For more information, please visit stemcell.com/cryostor.