How to Collect Plasma from Whole Blood Before Cell Isolation
Plasma and serum are two important components of whole blood that are widely used in both diagnostic and research applications. Plasma is the pale yellow, extracellular fluid that contains proteins, electrolytes, extracellular vesicles (EVs), and other soluble factors. It provides a cell-free medium rich in biomarkers for downstream analysis. Serum, obtained after blood coagulation, can also be used in many of the same assays.
Collecting plasma enables analysis of biomarkers such as proteins, nucleic acids, and EVs, while also preserving the cellular fraction of whole blood for further processing. Once the plasma is removed and replaced with the appropriate medium, the remaining cells, including peripheral blood mononuclear cells (PBMCs), can be isolated and characterized.
This protocol describes how to collect plasma from a whole blood sample and prepare the cellular fraction for downstream isolation of specific cell populations. Using technologies such as 贰补蝉测厂别辫鈩, 搁辞蝉别迟迟别厂别辫鈩, or density gradient centrifugation, immune cell populations can be efficiently enriched to support both diagnostic and discovery research.
Materials
- Whole blood sample collected with an anticoagulant
- Recommended medium according to your cell isolation kit鈥檚 Product Information Sheet. Recommended options include:
- 贰补蝉测厂别辫鈩 Buffer (Catalog #20144)
- RoboSep鈩 Buffer (Catalog #20104)
- Phosphate-buffered saline containing 2% fetal bovine serum (PBS + 2% FBS, Catalog #07905)
- Tube for plasma collection
Protocol
Part I. Plasma Collection
There are multiple options for generating or sourcing astrocytes:
- Collect whole blood using an appropriate anticoagulant.
- Centrifuge the blood at 2000 - 2500 脳 g for 10 - 15 minutes at room temperature (15 - 25掳C) with the brake off.
- Carefully aspirate the pale yellow plasma layer, leaving ~1 cm above the red blood cell pellet. To minimize disturbance of the cellular components and accidental collection, position the pipette tip at the liquid surface and gradually lower as plasma is collected.
- Transfer the collected plasma into a new, labeled collection tube.
Part II. Preparing the Blood Sample for Cell Isolation
- To continue with cell isolation from the residual blood sample, replace the removed plasma with an equal volume of the recommended medium. Please refer to the Product Information Sheet for your chosen cell isolation product to determine the appropriate medium.
- The replenished blood sample can now be treated as whole blood for your specific cell isolation protocol. For detailed sample processing instructions, please consult the Product Information Sheet of your selected product.
Note: The volume-replenished blood sample is suitable as the starting material for various applications, including 贰补蝉测厂别辫鈩 Direct kits, 贰补蝉测厂别辫鈩 Whole Blood kits, 搁辞蝉别迟迟别厂别辫鈩 kits, and density gradient PBMC preparation using products such as 厂别辫惭补迟别鈩 tubes and 尝测尘辫丑辞辫谤别辫鈩.
Explore 海角破解版鈥檚 complete portfolio of cell isolation products, optimized for use with whole blood, plasma-depleted blood, buffy coat, or PBMCs.
Learn more about efficient technologies for isolating cells from blood samples >
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