Inflammatory and Autoimmune Diseases
Inflammatory and autoimmune diseases are often caused by genetic and environmental factors that give rise to aberrant immune responses. Controlling host immune responses without eliminating the ability to control infections is crucial to managing these diseases.
Below is a collection of scientific resources for your inflammatory and autoimmune disease research.
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Frequencies of Cell Types in Human Peripheral Blood
This wallchart provides a list of the frequencies of cell types in human peripheral blood, as well as the column-free cell isolation kits available for each cell type.
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Isolating Cells from Atypical SamplesTechnical tip from our dedicated team of Product and Scientific Support specialists -
Using EasySepâ„¢ Positive Selection Kits for Cell DepletionThe EasySepâ„¢ system allows you to use a positive selection kit to deplete the labeled cell type, by making changes to the separation scheme of the protocol -
Considerations for Flow Cytometry GatingTo achieve accurate results, it is important to use an optimal gating strategy when assessing cell samples via flow cytometry -
Total Nucleated Cell and Viable Cell CountsAccurate cell counts are important in experiments. Ideally both the total nucleated cell (TNC) count and the viability of the cells should be assessed -
Refrigerate, Freeze, or Fix Cells for Flow Cytometry or StorageFind tips and solutions for storing, freezing, or fixing cells for later analysis or long term storage -
Processing Multiple Samples at One Time with the EasyEightsâ„¢ MagnetThe EasyEightsâ„¢ EasySepâ„¢ Magnet is for cell separation procedures using selected EasySepâ„¢ reagents when simultaneously processing multiple samples -
Isolate Multiple Cell Types from a Single Sample with Sequential IsolationSequential separation, for higher recovery of purified cells from limited sample size and isolation of multiple cell types, is ideal for chimerism analysis -
Assessment of Your Starting Sample and Calculation of Recovery from Cell Isolation ProceduresSetting aside a small portion of your starting sample allows you to assess the frequency of desired cells in the sample prior to isolation -
Frequently Asked Questions on Primary CellsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Isolate Cells with a Simple Pour-Off: EasySepâ„¢ Cell Separation TechnologyLearn how to perform the EasySepâ„¢ pour-off -
The Easy 50 EasySepâ„¢ Magnet: Portable Large-Volume Cell Separation In 25 MinutesLearn to use the portable yet powerful Easy 50 EasySepâ„¢ magnet to obtain purified cells from large volumes & a variety of sources -
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficollâ„¢ or Lymphoprepâ„¢)This technical guide demonstrates how to isolate peripheral blood mononuclear cells from whole blood using density gradient centrifugation
