Immuno-Oncology
Immune cells have the capability to eliminate tumor cells; however, tumor cells can develop the ability to evade immune responses. Current research efforts have been focused on developing personalized strategies for cancer treatment and developing new therapies that bolster the immune response in the fight against tumor cells.
Below is a collection of scientific resources for your immuno-oncology research.
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Production of Chimeric Antigen Receptor T Cells
This free Nature Protocols Wallchart summarizes the processes involved in producing CAR T cells for therapy.
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Reducing Platelet Contamination in Blood SamplesPlatelets (thrombocytes), cell fragments derived from megakaryocytes, are used in normal blood clotting, and must be handled gently to prevent activation -
Using Cell Density Gradients to Optimize Your Cell IsolationsRosetteSepâ„¢, a unique immunodensity cell isolation platform, isolates highly purified cells by density gradient centrifugation without columns or magnets -
Working with Small Sample Volumes or Low Start Cell Numbers Using the Purple EasySepâ„¢ and EasyPlateâ„¢ EasySepâ„¢ PlatformsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Preparing a Polymorphonuclear Cell Fraction from Whole Blood using Ammonium Chloride LysisTechnical tip from our dedicated team of Product and Scientific Support specialists -
Isolate Virtually Any Cell Type Using EasySepâ„¢ or EasySepâ„¢ Release Indirect Selection KitsUse any biotinylated, FITC-, PE- or APC-conjugated antibody to label your desired cells and mix with EasySepâ„¢ reagents to obtain highly purified cells -
Isolating Cells from Atypical SamplesTechnical tip from our dedicated team of Product and Scientific Support specialists -
Using EasySepâ„¢ Positive Selection Kits for Cell DepletionThe EasySepâ„¢ system allows you to use a positive selection kit to deplete the labeled cell type, by making changes to the separation scheme of the protocol -
Considerations for Flow Cytometry GatingTo achieve accurate results, it is important to use an optimal gating strategy when assessing cell samples via flow cytometry -
Total Nucleated Cell and Viable Cell CountsAccurate cell counts are important in experiments. Ideally both the total nucleated cell (TNC) count and the viability of the cells should be assessed -
Processing Multiple Samples at One Time with the EasyEightsâ„¢ MagnetThe EasyEightsâ„¢ EasySepâ„¢ Magnet is for cell separation procedures using selected EasySepâ„¢ reagents when simultaneously processing multiple samples -
Isolate Multiple Cell Types from a Single Sample with Sequential IsolationSequential separation, for higher recovery of purified cells from limited sample size and isolation of multiple cell types, is ideal for chimerism analysis -
Assessment of Your Starting Sample and Calculation of Recovery from Cell Isolation ProceduresSetting aside a small portion of your starting sample allows you to assess the frequency of desired cells in the sample prior to isolation
