Immuno-Oncology
Immune cells have the capability to eliminate tumor cells; however, tumor cells can develop the ability to evade immune responses. Current research efforts have been focused on developing personalized strategies for cancer treatment and developing new therapies that bolster the immune response in the fight against tumor cells.
Below is a collection of scientific resources for your immuno-oncology research.
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Production of Chimeric Antigen Receptor T Cells
This free Nature Protocols Wallchart summarizes the processes involved in producing CAR T cells for therapy.
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Working with Small Sample Volumes or Low Start Cell Numbers Using the Purple EasySepâ„¢ and EasyPlateâ„¢ EasySepâ„¢ PlatformsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Preparing a Polymorphonuclear Cell Fraction from Whole Blood using Ammonium Chloride LysisTechnical tip from our dedicated team of Product and Scientific Support specialists -
Isolate Virtually Any Cell Type Using EasySepâ„¢ or EasySepâ„¢ Release Indirect Selection KitsUse any biotinylated, FITC-, PE- or APC-conjugated antibody to label your desired cells and mix with EasySepâ„¢ reagents to obtain highly purified cells -
Isolating Cells from Atypical SamplesTechnical tip from our dedicated team of Product and Scientific Support specialists -
Using EasySepâ„¢ Positive Selection Kits for Cell DepletionThe EasySepâ„¢ system allows you to use a positive selection kit to deplete the labeled cell type, by making changes to the separation scheme of the protocol -
Considerations for Flow Cytometry GatingTo achieve accurate results, it is important to use an optimal gating strategy when assessing cell samples via flow cytometry -
Total Nucleated Cell and Viable Cell CountsAccurate cell counts are important in experiments. Ideally both the total nucleated cell (TNC) count and the viability of the cells should be assessed -
Processing Multiple Samples at One Time with the EasyEightsâ„¢ MagnetThe EasyEightsâ„¢ EasySepâ„¢ Magnet is for cell separation procedures using selected EasySepâ„¢ reagents when simultaneously processing multiple samples -
Isolate Multiple Cell Types from a Single Sample with Sequential IsolationSequential separation, for higher recovery of purified cells from limited sample size and isolation of multiple cell types, is ideal for chimerism analysis -
Assessment of Your Starting Sample and Calculation of Recovery from Cell Isolation ProceduresSetting aside a small portion of your starting sample allows you to assess the frequency of desired cells in the sample prior to isolation -
Frequently Asked Questions on Primary CellsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Non-Viral CRISPR Knock-In Anti-B7-H3 CAR-T Cells Are Amenable for Treatment of Subtypes of Small Cell Lung CancerIn this webinar, Scientist Vimal Keerthi discusses his work on identifying CD276 (B7-H3) overexpression in primary human SCLC and developing a non-viral CRISPR-Cas9 knock-in (CKI) based platform against B7-H3 to manufacture CAR T cells for the treatment of SCLC. He demonstrates the feasibility of this CAR T manufacturing platform and how this provides a blueprint for immediate clinical translation, overcoming the bottleneck of viral vector production.<br><br>This webinar is part of the “Translational T Cell Talks: Scaling for the Future†event hosted in collaboration with Scientist.com on June 11, 2024. <a href="/translational-t-cell-talks-2024">View the rest of the talks and read the Q&A transcripts.</a>
