Cells of the Immune System
The immune system is comprised of many subsets of cells that work together to protect the host against infections and malignancies. Each one of these cells has a role to play. Research on how they work and their interactions with other subsets continues to reveal opportunities for novel therapeutic strategies.
Below is a collection of scientific resources for your immune cell research.
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Fast and Easy Cell Isolation with EasySep™
EasySep™ is a fast and easy immunomagnetic cell separation technology that allows you to isolate virtually any cell types from a variety of sample sources in as little as 8 minutes. This video describes the EasySep™ technology.
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Loading the RoboSepâ„¢-S Carousel and Starting a RunBasic overview on how to use and maintain your RoboSepâ„¢-S; steps to load the instrument with samples and reagents and start a cell separation run -
Acquiring DNA or RNA from Positively Selected CellsCells positvely selected with the EasySepâ„¢ system do not interfere with most downstream applications -
How to Isolate Nucleated Cells from Whole Blood Using HetaSepâ„¢ Erythrocyte Aggregation AgentHetaSepâ„¢ is an erythrocyte aggregation agent used to quickly separate nucleated cells from red blood cells -
Before Starting a Run on RoboSepâ„¢-SBasic overview on how to use and maintain your RoboSepâ„¢-S; steps to follow before starting a cell separation run -
How to Isolate Cells with EasySepâ„¢ Column-Free Cell Separation TechnologyIsolation of human CD4+ T cells by negative selection using EasySepâ„¢ to separate highly purified cells in as little as 8 minutes -
Isolate Virtually Any Cell Type Using EasySepâ„¢ or EasySepâ„¢ Release Indirect Selection KitsUse any biotinylated, FITC-, PE- or APC-conjugated antibody to label your desired cells and mix with EasySepâ„¢ reagents to obtain highly purified cells -
Using Cell Density Gradients to Optimize Your Cell IsolationsRosetteSepâ„¢, a unique immunodensity cell isolation platform, isolates highly purified cells by density gradient centrifugation without columns or magnets -
Using EasySepâ„¢ Positive Selection Kits for Cell DepletionThe EasySepâ„¢ system allows you to use a positive selection kit to deplete the labeled cell type, by making changes to the separation scheme of the protocol -
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficollâ„¢ or Lymphoprepâ„¢)This technical guide demonstrates how to isolate peripheral blood mononuclear cells from whole blood using density gradient centrifugation -
How to Count Cells Using a HemocytometerHow to prepare and count stained cell samples using a hemocytometer for both total nucleated cell counts and viable cell counts -
Bhushan Dharmadhikari, PhDDr. Bhushan Dharmadhikari shares his work studying dendritic cells and their applications in cancer immunology and immunotherapy -
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySepâ„¢ DirectIntroduction to the EasySepâ„¢ Direct immunomagnetic cell separation platform
