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Figure 1. Frozen Human Peripheral Blood Leukopaks Thawed Using the ThawSTAR® CB Automated Thawing System Show High Recovery and Viability for Pre-wash Assessment

Frozen Human Peripheral Blood Leukopaks (Quarter Size; Catalog #200-0132) were retrieved from liquid nitrogen and thawed using either ThawSTAR® CB Automated Thawing System with appropriately selected thawing settings or a water bath. When thawed using the ThawSTAR® CB Automated Thawing System or a water bath, (A) the mean live cell recovery was 4.42 x 10⁹ vs. 3.58 x 10⁹ cells for Donor 1, 4.53 x 10⁹ vs. 4.95 x 10⁹ cells for Donor 2, 3.94 x 10⁹ vs. 4.52 x 10⁹ cells for Donor 3, respectively. (B) The mean viability of automatic vs. water bath-thawed cells was 93.3% vs. 95.4% for Donor 1, 98.9% vs. 98.3% for Donor 2, and 98.2% vs. 98.5% for Donor 3, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a NucleoView™ counter.

Figure 2. Frozen CD4+ Cells Thawed Using the ThawSTAR® CB Automated Thawing System Show High Recovery and Viability for Pre-wash Assessment

CD4+ cells cryopreserved in CryoStor® CS10 (Catalog #07930) were retrieved from liquid nitrogen and thawed using either ThawSTAR® CB Automated Thawing System with appropriately selected thawing settings or a water bath. When thawed using the ThawSTAR® CB Automated Thawing System or a water bath, (A) the mean live cell recovery was 1.27 x 10⁹ vs. 1.19 x 10⁹ cells for Donor 1, 1.03 x 10⁹ vs. 1.16 x 10⁹ cells for Donor 2, respectively and (B) the mean viability was 92.0% vs. 93.3% for Donor 1, 95.2% vs. 97.4% for Donor 2, respectively. The cells were from 2 different donors and were tested in triplicates. Cell recovery and viability were assessed using a NucleoView™ counter.