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Neurons in Just Five Days
Rapidly and efficiently generate forebrain neurons from human pluripotent stem cells (hPSCs) in just five days using the STEMdiff鈩-TF Forebrain Induced Neuron Differentiation Kit. This forward programming system uses lipid nanoparticle (LNP)-based delivery of non-integrating Neurogenin-2 (NGN2) mRNA to drive transcription factor鈥搈ediated differentiation, producing highly pure excitatory glutamatergic neurons with forebrain identity.
For researchers looking to extend their studies, these induced neurons can be matured with the STEMdiff鈩 Forebrain Neuron Maturation Kit (Catalog #08605). Tested across multiple hPSC lines, this streamlined workflow delivers consistent, functional human-derived neurons, empowering in vitro modeling of neurological disease mechanisms, advancing drug discovery efforts, and helping reduce reliance on animal models.
Rapidly Generate Highly Pure Excitatory Glutamatergic Forebrain Neuron Cultures
With the STEMdiff鈩-TF Forebrain Induced Neuron Differentiation Kit, find confidence in consistent, high-quality results.
Generate Highly Pure Neuron Cultures Using the STEMdiff鈩-TF Forebrain Induced Neuron Differentiation Kit
(A) Representative ICC images of neurons differentiated from H9 hESC line and Healthy Control Human iPSC Line, Female, SCTi003-A (Catalog #200-0511) on Day 5 of neuronal differentiation with the STEMdiff鈩-TF Forebrain Induced Neuron Kit show widespread neuronal induction, with cells stained for TUBB3 (green), MAP2 (magenta), SYN1 (red), and nuclei labeled with DAPI (blue). Scale bars = 100 碌m. (B) Quantification of ICC data of pan-neuronal markers on Days 5 and 21. Neurons generated with the STEMdiff鈩-TF Forebrain Induced Neuron Kit exhibit high expression of TUBB3 at Day 5 and increasing expression of intermediate/maturing (MAP2), and late/pre-synaptic (SYN1) markers by Day 21, consistent with progressive neuronal maturation. Data was collected from four hPSC lines, with 4 - 8 replicates per line. Bar graphs show mean 卤 SD. ICC = immunocytochemistry; hESC = human embryonic stem cell; iPSC = induced pluripotent stem cell
Why Use the STEMdiff鈩-TF Forebrain Induced Neuron Differentiation Kit?
- Achieve rapid generation of functional neurons from hPSCs in just five days
- Generate a highly pure excitatory glutamatergic neuron population through NGN2 overexpression with no risk of genomic integration
- Ensure consistent, reproducible results across multiple hPSC lines
- Pair with the STEMdiff鈩 Forebrain Neuron Maturation Kit with integrated BrainPhys鈩 media to support neuronal activity for predictive models
