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STEMdiffâ„¢ Cardiomyocyte Plating Supplement

A specialized supplement for improving plating efficiency post-thawing and post-harvesting of human pluripotent stem cell-derived cardiomyocytes

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STEMdiffâ„¢ Cardiomyocyte Plating Supplement

A specialized supplement for improving plating efficiency post-thawing and post-harvesting of human pluripotent stem cell-derived cardiomyocytes

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A specialized supplement for improving plating efficiency post-thawing and post-harvesting of human pluripotent stem cell-derived cardiomyocytes
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Product Advantages


  • Improve plating efficiency and double the number of hPSC-derived cardiomyocytes available for your experiments

  • Retain the stable electrical profile of hPSC-derived cardiomyocytes following replating

  • Save time and money with more efficient cardiomyocyte replating after thawing and dissociation

Overview

Double the yield of human pluripotent stem cell (hPSC)-derived cardiomyocytes following dissociation, thawing, and replating by using the STEMdiffâ„¢ Cardiomyocyte Plating Supplement. When used with STEMdiffâ„¢ Cardiomyocyte Support Medium, STEMdiffâ„¢ Cardiomyocyte Plating Supplement improves plating efficiency and minimizes the loss of hPSC-derived cardiomyocytes.

This product is designed for hPSC-derived cardiomyocytes generated using STEMdiffâ„¢ Ventricular Cardiomyocyte Differentiation Kit or STEMdiffâ„¢ Atrial Cardiomyocyte Differentiation Kit. By doubling your cardiomyocyte plating efficiency, the STEMdiffâ„¢ Cardiomyocyte Plating Supplement can help increase your experimental throughput.

Use this product as part of a complete, optimized workflow including these compatible STEMdiffâ„¢ cardiomyocyte products:

STEMdiffâ„¢ Ventricular Cardiomyocyte Differentiation Kit
STEMdiffâ„¢ Atrial Cardiomyocyte Differentiation Kit
STEMdiffâ„¢ Cardiomyocyte Dissociation Kit
STEMdiffâ„¢ Cardiomyocyte Support Medium
STEMdiffâ„¢ Cardiomyocyte Freezing Medium
STEMdiffâ„¢ Cardiomyocyte Maintenance Kit
Contains
Serum
Cell Type
Cardiomyocytes, PSC-Derived
Species
Human
Application
Cryopreservation, Differentiation, Maintenance
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology, Cell Therapy Development

Data Figures

Graphs demonstrating iImproved plating: STEMdiffâ„¢ Cardiomyocyte Plating Medium (51%) vs. Support Medium (16%).

Figure 1. Increased Plating Efficiency Observed When Using hPSC-Derived Cardiomyocytes Using STEMdiffâ„¢ Cardiomyocyte Plating Kit

Plating efficiency of hPSC-derived cardiomyocytes was assessed 1 day after replating at low density in either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. Across 4 hPSC lines, plating efficiency was improved when cardiomyocytes were replated in STEMdiff™ Cardiomyocyte Plating Medium (51% ± 3%, n=15) compared to STEMdiff™ Cardiomyocyte Support Medium (16% ± 1%, n=15).

Microscope images showing enhanced attachment of replated hPSC-derived cardiomyocytes using plating medium.

Figure 2. Morphology of Replated hPSC-Derived Cardiomyocytes Using STEMdiffâ„¢ Cardiomyocyte Plating Kit

Representative images of replated hPSC-derived cardiomyocytes. hPSC-derived cardiomyocytes were replated at low density in either STEMdiffâ„¢ Cardiomyocyte Support Medium or STEMdiffâ„¢ Cardiomyocyte Plating Medium. 1 day after replating, hPSC-derived cardiomyocytes exhibit improved attachment when replated with STEMdiffâ„¢ Cardiomyocyte Plating Medium.

Microelectrode array shows stable beat rate of cardiomyocytes replated with this kit.

Figure 3. hPSC-Derived Cardiomyocytes Replated Using STEMdiffâ„¢ Cardiomyocyte Plating Kit Exhibit a Stable Beat Profile

Microelectrode array recordings of hPSC-derived cardiomyocytes replated using either STEMdiffâ„¢ Cardiomyocyte Support Medium or STEMdiffâ„¢ Cardiomyocyte Plating Medium. Cardiomyocytes were maintained for 1 week with STEMdiffâ„¢ Cardiomyocyte Maintenance Medium. Across 4 hPSC lines, cardiomyocytes retain a stable beat rate when replated using STEMdiffâ„¢ Cardiomyocyte Plating Medium.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
100-1120
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-1120
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-1120
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT º£½ÇÆƽâ°æ, REFER TO WWW.º£½ÇÆƽâ°æ.COM/COMPLIANCE.

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