Propidium Iodide (PI) | DNA-Labeling Dye | ��ƽ�� Technologies

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Propidium Iodide

Cell viability dye (DNA-labeling dye)

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Propidium Iodide

Cell viability dye (DNA-labeling dye)

Size

10 mg Request Pricing

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Cell viability dye (DNA-labeling dye)

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Overview

Propidium Iodide (PI) is a red-fluorescent cell viability dye which is excluded from live cells with intact membranes, but penetrates dead or damaged cells and binds to DNA and RNA by intercalating between the bases. It is widely used as a counterstain to differentiate and exclude non-viable cells in flow cytometric analyses, and can be excited using blue (488 nm), green (532 nm), or yellow-green (561 nm) laser lines, with detection in the FL2 or FL3 channels. PI is used in DNA fluorescence imaging applications to discriminate early and late stages of apoptosis, to study cell‐mediated cytotoxicity, and for chromosome analysis. It is also commonly used in quantitative DNA assays.

3,8-Diamino-5-{3-[diethyl(methyl)ammonio]propyl}-6-phenylphenanthridinium diiodide; PI; Propidium diiodide

Other

Human, Mouse, Non-Human Primate, Other, Rat

Flow Cytometry

Immunology, Neuroscience, Stem Cell Biology

25535-16-4

C₂₇H₃₄N₄ · 2I

668.4 g/mol

Data Figures

Figure 1. (A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Propidium Iodide

(B) Chemical structure of Propidium Iodide.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Name

Catalog #

75002

Lot #

All

Language

English

Document Type

Product Name

Catalog #

75002

Lot #

All

Language

English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area

Workflow Stages

Workflow Stages for B Cell Research

Cell Sourcing

Cell Engineering

Cell Isolation

Cell Characterization

Cell Expansion and Differentiation

Workflow Stages for Dendritic Cell Research

Sample Preparation

Cell Sourcing

Cell Isolation