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MSC Characterization Antibody Panel

Multi-color flow cytometry kit for characterization of human mesenchymal stromal cells

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MSC Characterization Antibody Panel

Multi-color flow cytometry kit for characterization of human mesenchymal stromal cells

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Multi-color flow cytometry kit for characterization of human mesenchymal stromal cells
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Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer for use with EasySep™ cell separation kits

  2. Corning® 96-Well Round-Bottom Microplate
    Corning® 96-Well Round-Bottom Microplate

    Clear polystyrene round-bottom untreated microplate with lid

  3. Anti-Human CD32 Antibody, Clone IV.3
    Anti-Human CD32 Antibody, Clone IV.3

    Mouse monoclonal IgG2b antibody against human CD32

  4. DAPI (Hydrochloride)
    DAPI (Hydrochloride)

    DNA-labeling dye

Overview

Verify mesenchymal stromal cell (MSC) identity quickly and confidently using the MSC Characterization Antibody Panel. This ready-to-use, flow cytometry-compatible panel includes three fluorochrome-conjugated antibodies (CD73, CD90, CD105) for MSC identification and one (CD45) for exclusion of non-MSC populations.

Validated across MSCs derived from bone marrow, adipose tissue, umbilical cord, dental pulp, and human pluripotent stem cell (hPSC)-derived mesenchymal progenitors, the panel enables reproducible, single-step assessment of MSC identity for both RUO and GMP-adjacent workflows. Pair with the MesenCult™-ACF Plus Culture Kit to ensure consistent expansion conditions and phenotype validation across your MSC workflow.

This panel can be used as part of a complete MesenCult™ MSC cell culture portfolio that includes reagents for differentiation, culture, and characterization of MSCs. You can also start your experiments with a validated pure MSC culture using Human iPSC-Derived Mesenchymal Progenitor Cells.
Subtype
Primary Antibodies
Cell Type
Mesenchymal Stromal Cells
Application
Flow Cytometry
Area of Interest
Endothelial Cell Biology, Stem Cell Biology

Data Figures

Flow cytometry dot plots comparing the difference in expression of CD45, CD73, CD90, and CD105 in human bone marrow-derived mesenchymal stromal cells (MSCs) labeled with the hMSC CD45/CD73/CD90/CD105 Antibody Panel and cultured either in MesenCult™-ACF Plus Medium or in serum-containing medium.

Figure 1. Flow Cytometry Analysis of Human Bone Marrow-Derived Mesenchymal Stromal Cells Labeled Using the hMSC CD45/CD73/CD90/CD105 Antibody Panel

(A) When cultured in MesenCult™-ACF Plus Medium, the labeled human bone marrow (BM)-derived mesenchymal stromal cells (MSCs), demonstrated positive expression of CD73, CD105, and CD90 as well as negative expression of CD45 at P0. (B) When cultured in serum-containing medium, the BM-derived MSCs exhibit reduced CD73, CD105, and CD90 expression as well as increased CD45 expression at P0 due to contaminating CD45+ cells.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
100-0354
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-0354
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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Workflow Stages

Resources and Publications

Educational Materials (2)

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