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Streamline your assays with ready-to-use, ethically sourced, mobilized primary CD34+ cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications.
Donors are mobilized with:
鈥 G-CSF: a maximum of 10 碌g/kg/day of granulocyte colony-stimulating factor for 3 - 5 days prior to collection (Catalog #70060)
鈥 Plerixafor: a maximum of 0.24 mg/kg plerixafor for 1 day prior to collection (Catalog #70075)
鈥 G-CSF and Plerixafor: a maximum of 10 碌g/kg/day of G-CSF for 3 - 5 days prior to collection and a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection (Catalog #70073)
Isolated from leukapheresis samples using positive immunomagnetic separation techniques and cryopreserved in serum-free medium, cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above, after selecting from the product options. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.
Certain products are only available in select territories. Please contact your local sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.
Figure 1. StemSpan鈩 HSC Plus Supplement Supports Expansion of Mobilized Peripheral Blood-Derived Primitive HSPCs Across Different StemSpan鈩 Media
Purified CD34+ cells derived from G-CSF mobilized peripheral blood (mPB) were cultured at a concentration of 10,000 cells per mL in StemSpan鈩 media (SFEM, SFEM II, XF, and AOF). Cultures were supplemented with StemSpan鈩 CD34 Expansion Supplement alone (grey bars) or together with StemSpan鈩 HSC Plus Supplement (orange bars). After 7 days of culture, flow cytometry was used to analyze the (A) total nucleated cell (TNC) expansion, (B) expansion of CD34+ cell subsets, and (C) the frequency of CD34+ HSPC subsets: CD34+, CD34+CD45RA-CD90+ and CD34+CD45RA-CD90+EPCR+CD133+. Across all tested StemSpan鈩 media, StemSpan鈩 HSC Plus Supplement promoted greater expansion of CD34+CD45RA-CD90+ and CD34+CD45RA-CD90+CD133+EPCR+ cells compared to StemSpan鈩 CD34+ Expansion Supplement alone. Each subsequent cell subset was progressively more enriched for phenotypic stem/progenitor cells. Data shown are mean 卤 SEM (n=6).
Figure 2. CellPore鈩 Transfection System Enables Robust Knock-Out in HSPCs Derived from Mobilized Peripheral Blood Across a Wide Range of Cell Numbers
Cryopreserved CD34+ HSPCs isolated from mobilized peripheral blood were cultured for 24 hours in complete StemSpan鈩 SFEM II culture medium. RNP complexes targeting the B2M gene were delivered to a range of cell numbers per reaction. Four days post-transfection, cell viability (A) and surface MHC-I marker expression, indicating editing efficiency (B) were measured via flow cytometry in bulk CD34+ HSPCs and primitive CD34+CD45RA-CD90+ HSCs. Comparable viability and editing efficiency were obtained across the cell number range tested. Data are shown as mean 卤 SD, n = 2 - 5.
Figure 3. Cryopreserved G-CSF Mobilized Human Peripheral Blood CD34+ Cells Generate Hematopoietic Colonies in CFU Assays
Cryopreserved G-CSF Mobilized Human Peripheral Blood CD34+ Cells (Catalog #70060) were thawed, plated at a concentration of 5 x 10^2 viable cells/dish and cultured in MethoCult鈩 Optimum Medium (Catalog #04034) for 14 days. Shown are colonies produced by hematopoietic progenitor cells in the sample, imaged with STEMvision鈩.
This product is designed for use in the following research area(s) as part
of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we
offer to support each research area.
Mobilized Human Peripheral Blood CD34+ Cells, Frozen
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT 海角破解版, REFER TO WWW.海角破解版.COM/COMPLIANCE.
